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Food Industry FAMEs on Rt-2560 by AOCS Method Ce-1j-07 Using H2 (Optimized Method)

PeakstR (min)Conc. (µg/mL)Structural Nomenclature
1.Methyl caproate4.740400C6:0
2.Methyl octanoate4.953400C8:0
3.Methyl decanoate5.317400C10:0
4.Methyl undecanoate5.587200C11:0
5.Methyl dodecanoate5.939400C12:0
6.Methyl tridecanoate6.398200C13:0
7.Methyl myristate6.998400C14:0
8.Methyl myristoleate7.736200C14:1 (c9)
9.Methyl pentadecanoate7.781200C15:0
10.Methyl pentadecenoate8.734200C15:1 (C10)
11.Methyl palmitate8.806600C16:0
12.Methyl palmitoleate9.831200C16:1 (c9)
13.Methyl heptadecanoate10.136200C17:0
14.Methyl heptadecenoate11.448200C17:1 (c10)
15.Methyl stearate11.882400C18:0
16.Methyl octadecenoate12.877200C18:1 (t9)
17.Methyl oleate13.345400C18:1 (c9)
18.Methyl linolelaidate14.685200C18:2 (t9,t12)
PeakstR (min)Conc. (µg/mL)Structural Nomenclature
19.Methyl linoleate15.928200C18:2 (c9,c12)
20.Methyl arachidate17.136400C20:0
21.Methyl linolenate18.252200C18:3 (c6,c9,c12)
22.Methyl eicosenoate19.422200C20:1 (c11)
23.Methyl linolenate19.740200C18:3 (c9,c12,c15)
24.Methyl heneicosanoate20.592200C21:0
25.Methyl eicosadienoate21.621200C20:2 (c11,c14)
26.Methyl behenate22.380400C22:0
27.Methyl eicosatrienoate22.884200C20:3 (c8,c11,c14)
28.Methyl erucate23.510200C22:1 (c13)
29.Methyl eicosatrienoate23.656200C20:3 (c11,c14,c17)
30.Methyl arachidonate23.901200C20:4 (c5,c8,c11,c14)
31.Methyl tricosanoate24.240200C23:0
32.Methyl docosadienoate25.408200C22:2 (c13,c16)
33.Methyl eicosapentaenoate26.293200C20:5 (c5,c8,c11,c14,c17)
34.Methyl lignocerate26.293400C24:0
35.Methyl nervonate27.653200C24:1 (c15)
36.Methyl docosahexaenoate33.053200C22:6 (c4,c7,c10,c13,c16,c19)
ColumnRt-2560, 100 m, 0.25 mm ID, 0.20 µm (cat.# 13198)
Standard/SampleFood industry FAME mix (cat.# 35077)
Conc.:10,000 µg/mL total concentration
Inj. Vol.:1 µL split (split ratio 20:1)
Liner:Topaz 4.0 mm ID Precision inlet liner w/wool (cat.# 23305)
Inj. Temp.:250 °C
Oven Temp.:180 °C (hold 19.8 min) to 215 °C at 32.3 °C/min (hold 11 min)
Carrier GasH2, constant flow
Flow Rate:2.5 mL/min
DetectorFID @ 250 °C
Constant Column + Constant Make-up:52 mL/min
Hydrogen flow:40 mL/min
Air flow:400 mL/min
Data Rate:50 Hz
InstrumentAgilent 7890A GC
NotesMethod optimized from He using the EZGC method translator (
C4:0 Methyl butyrate (623-42-7) elutes in the solvent front.
Resolution of critical pair cis-11-C20:1 (#22) and cis-9,12,15-C18:3 (#23) is 2.03.