Food Industry FAME on FAMEWAX by EN14103 (2011)

GC_PC1332

	Peaks	t_R (min)	Conc. (mg/mL)	Structural Nomenclature
1.	Methyl capronate	3.629	1.2	C6:0
2.	Methyl caprylate	6.237	1.2	C8:0
3.	Methyl caprate	8.787	1.2	C10:0
4.	Methyl undecanoate	9.971	0.6	C11:0
5.	Methyl laurate	11.105	1.2	C12:0
6.	Methyl tridecanoate	12.179	0.6	C13:0
7.	Methyl myristate	13.215	1.2	C14:0
8.	Methyl myristoleate	13.549	0.6	C14:1 (cis-9)
9.	Methyl pentadecanoate	14.196	0.6	C15:0
10.	Methyl pentadecenoate	14.524	0.6	C15:1 (cis-10)
11.	Methyl palmitate	15.152	1.8	C16:0
12.	Methyl palmitoleate	15.355	0.6	C16:1 (cis-9)
13.	Methyl margarate	16.052	0.6	C17:0
14.	Methyl heptadecenoate	16.261	0.6	C17:1 (cis-10)
15.	Methyl stearate	16.995	1.2	C18:0
16.	Methyl oleate	17.156	1.2	C18:1 (cis-9)
17.	Methyl elaidate	17.168	1.2	C18:1 (trans-9)
18.	Methyl linoleate	17.583	0.6	C18:2 (all-cis-9,12)

	Peaks	t_R (min)	Conc. (mg/mL)	Structural Nomenclature
19.	Methyl linolelaidate	17.641	0.6	C18:2 (all-trans-9,12)
20.	Methyl γ-linolenate	17.874	0.6	C18:3 (all-cis-6,9,12)
21.	Methyl nonadecanoate	18.052	2.0	C19:0
22.	Methyl α-linolenate	18.223	0.6	C18:3 (all-cis-9,12,15)
23.	Methyl arachidate	19.075	1.2	C20:0
24.	Methyl (Z)-11-eicosenoate	19.255	0.6	C20:1 (cis-11)
25.	Methyl 11,14-eicosadienoate	19.761	0.6	C20:2 (all-cis-11,14)
26.	Methyl eicosa-8,11,14-trienoate	20.046	0.6	C20:3 (all-cis-8,11,14)
27.	Methyl heneicosanoate	20.197	0.6	C21:0
28.	Methyl arachidonate	20.290	0.6	C20:4 (all-cis-5,8,11,14)
29.	Methyl 11,14,17-eicosatrienoate	20.488	0.6	C20:3 (all-cis-11,14,17)
30.	Methyl 5,8,11,14,17-eicosapentanoate	21.036	0.6	C20:5 (all-cis-5,8,11,14,17)
31.	Methyl behenate	21.39	1.2	C22:0
32.	Methyl erucate	21.595	0.6	C22:1 (cis-13)
33.	Methyl docosadienoate	22.150	0.6	C22:2 (all-cis-13,16)
34.	Methyl tricosanoate	22.584	0.6	C23:0
35.	Methyl lignocerate	23.826	1.2	C24:0
36.	Methyl docosahexaenoate	23.863	0.6	C22:6 (all-cis-4,7,10,13,16,19)
37.	Methyl nervonate	24.055	0.6	C24:1 (cis-15)

Injection
Column	FAMEWAX, 30 m, 0.25 mm ID, 0.25 µm (cat.# 12497)
Standard/Sample	Food industry FAME mix (cat.# 35077)
	Methyl nonadecanoate (cat.# 35055)
Diluent:	Standard cat.# 35055 was dissolved in toluene.
Inj. Vol.:	1 µL split (split ratio 100:1)
Liner:	Topaz 4.0 mm ID Precision inlet liner w/wool (cat.# 23305)
Inj. Temp.:	240 °C
Oven
Oven Temp.:	60 °C (hold 2 min) to 200 °C at 10 °C/min to 240 °C at 5 °C/min (hold 7 min)
Carrier Gas	H₂, constant flow
Flow Rate:	1.7 mL/min

Detector	FID @ 250 °C
Instrument	Agilent 7890B GC
Notes	This chromatogram is an overlay of two injections: food industry FAME standard (black) and C19:0 methyl ester in toluene (red). An excellent separation of C19:0 (used in EN 14103 as an internal standard) and the most prevalent FAMEs found in biodiesel blends was achieved. Note that C4:0 from the food industry FAME standard elutes in the solvent front.

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