Warfarin and Chemotherapeutic Drugs on Raptor FluoroPhenyl by LC-MS/MS

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion	Product Ion
1.	Carboplatin	0.83	100	372.00	294.00	355.00
2.	5-Fluorouracil	0.89	100	130.98	113.99	57.99
3.	Methotrexate	2.18	20	455.15	308.09	175.14
4.	Ifosfamide	2.67	20	261.03	91.95	153.90
5.	Cyclophosphamide	2.75	20	261.03	139.97	106.01
6.	Etoposide	3.10	100	589.34	229.01	185.03
7.	Warfarin	3.58	10	309.11	162.99	251.01

Column

Raptor FluoroPhenyl (cat.# 9319A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor FluoroPhenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9319A0252)

Temp.:

50 °C

Standard/Sample

Diluent:

Water:methanol (95:5)

Conc.:

10-100 ng/mL

Inj. Vol.:

10 µL

Mobile Phase

0.1% Formic acid in water

Methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	95	5
4.00	0.4	0	100
4.01	0.4	95	5
6.00	0.4	95	5

Max Pressure:

400 bar

Detector	MS/MS
Ion Mode:	ESI+
Mode:	MRM
Instrument	UHPLC
Sample Preparation	The sample solution was prepared by protein precipitation using Resprep PPT³ 96-well plates (cat# 26489) and reservoir (cat# 26493). A 200 μL aliquot of fortified human plasma was mixed with 600 μL of acetonitrile by vortexing for 30 seconds at 2000 rpm. The sample was filtered using a vacuum manifold. The filtrate was dried and reconstituted with 100 μL of water:methanol (95:5) and injected for analysis.

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