Featured Pittcon Short Course: Practical Maintenance and Troubleshooting in Gas Chromatography

Jaap de Zeeuw

Jaap de Zeeuw

Date: Tuesday, March 03, 2020

Location: Pittcon 2020, Chicago

Course Description: 

In gas chromatography, 90% of the problems encountered can be traced back to issues in the injection system. In this half-day featured Pittcon short course, Restek’s Jaap de Zeeuw will discuss the purpose and impact of the critical parts used in split and splitless injection, as well as how they affect overall system maintenance schedules. Carrier gas choice and purity, tubing, connections, septa, ferrules, seals, liners, column coupling, column installation, and column maintenance all will be covered in detail. In addition, Jaap will discuss column operation/optimization concepts and explain how to extend GC column lifetime most effectively. At the end, we will explore a series of practical examples together through troubleshooting exercises.

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Webinar: How to Analyze Extended PFAS Lists Including Ultrashort-Chain (C2-C3) PFAS

Register today for this timely C&EN webinar about PFAS analysis and incorporating ultrashort-chain PFAS into an existing method for legacy and alternative compounds. Mike Chang, Global Environmental Market Business Development Manager at Restek, will discuss LC-MS/MS method development for C2 and C3 PFAS analysis and analytical methodologies for simultaneous chromatographic determination of ultrashort-chain, alternative, and legacy PFAS.

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Remove Both Phospholipids and Proteins in One Resprep PLR SPE Procedure

Simultaneously remove phospholipids and proteins in a single, simple procedure with new Resprep PLR (phospholipid removal) SPE products. Whole blood, serum, and plasma all contain proteins and phospholipids that can interfere with target analytes and hasten the need for instrument maintenance. It’s important to remove them from samples prior to analysis to avoid signal suppression, and Resprep PLR SPE cartridges or 96-well plates make this an easy task by combining protein precipitation and phospholipid removal in one sample preparation process. No analyte-specific method development is required because the same procedure can be used for samples containing acids, bases, or neutral compounds. In addition, effective removal of phospholipids and proteins from sample extracts reduces contamination, minimizing the frequency of instrument maintenance.


Phospholipid and Protein Removal in One Simple SPE Process Prevents Matrix-Based Signal Suppression

Published By: Restek Corporation

Year of Publication: 2020

Link: https://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/gen_GNAR3124-UNV

Abstract: Biological samples contain proteins and phospholipids that can interfere with LC-MS/MS analysis and increase the frequency of instrument maintenance. Here we used Resprep PLR SPE to simultaneously remove proteins and phospholipids from plasma in one high-efficiency sample preparation process. Concurrent phospholipid removal eliminated signal suppression and excellent recoveries were obtained for a wide range of chemical classes without the need for additional method development.

Eliminate Cross-Contamination with New Resprep Quick-Replace Vacuum Manifolds

Cross-contamination of samples run in series through typical SPE vacuum manifolds is a common problem for sample preparation labs. New Resprep Quick-Replace vacuum manifolds eliminate this risk because they contain a disposable valve liner that can be quickly and easily replaced between samples, ensuring a clean flow path for every extraction. In addition, the screw-type valve design provides precise, independent flow control of each SPE port. Resprep Quick-Replace vacuum manifolds are compatible with any standard male luer SPE cartridge and are available in 12- and 24-port models. The easily configurable rack system supports a wide variety of collection vessels and can be customized to accommodate vessel size.


Fast, Low-Solvent Analysis of Cannabinoids Increases Lab Productivity and Decreases Solvent Costs

Published By: Restek Corporation

Year of Publication: 2019

Link: https://www.restek.com/Technical-Resources/Technical-Library/Foods-Flavors-Fragrances/fff_FFSS3123-UNV

Abstract: Suitable for high-throughput cannabis testing labs, this LC-UV method reliably separates 19 cannabinoids in a quick, 10-minute analysis. Using only 3 mL of acetonitrile per run, labs can significantly decrease solvent use and the associated costs by adopting this procedure.

Improve Acrylamide Analysis with a Faster, Longer-Lasting LC Column

The porous graphitized carbon (PGC) columns typically used for acrylamide analysis by method EN 16618:2015 provide adequate retention of acrylamide and sufficient separation from matrix components, but they are subject to long run times and short column lifetimes. Switching to an Allure Acrylamide LC column is a better alternative: an Allure Acrylamide column used with a deuterated internal standard provides faster total analysis times and meets system suitability requirements longer.

Allure Acrylamide columns meet the EN method 1.7-minute retention time requirement with good separation from matrix components and a much shorter equilibration time. A per analysis savings of 2.5 minutes allows more samples to be analyzed per shift, significantly increasing lab output. In addition to faster run times, testing showed that the Allure Acrylamide column passed the 1.7-minute system suitability requirement for over twice as many injections as a typical PGC column.

Developed to withstand 100% aqueous mobile phases and elute coextracted matrix components rather than strongly retaining them, Restek Allure Acrylamide guard and analytical columns deliver the same robust performance injection to injection and column to column, making them a more reliable choice for acrylamide analysis, even in difficult food matrices like potato chips or coffee.


Improve Acrylamide Analysis with a Long-Lasting LC Column and a Cost-Effective Internal Standard

Published By: Restek Corporation

Year of Publication: 2019

Link: https://www.restek.com/Technical-Resources/Technical-Library/Foods-Flavors-Fragrances/fff_FFAR3126-UNV

Abstract:The porous graphitized carbon (PGC) columns typically used for acrylamide analysis by method EN 16618.2015 provide adequate retention of acrylamide and sufficient separation from matrix components, but they are subject to short column lifetimes and the need for extensive sample preparation. Here, we demonstrate an approach using an Allure Acrylamide LC column and deuterated internal standard that provides faster total analysis times and meets system suitability requirements longer, allowing labs to significantly increase sample throughput.

New Rxi-65TG Columns Ensure Accurate, Reliable GC Analysis of Triglycerides in Edible Oils

With best-in-class thermal stability, new Rxi-65TG columns from Restek are the top choice for accurate, reliable analysis of triglycerides (acylglycerols) in edible oils. Novel chemistry and cutting-edge manufacturing allow Rxi-65TG columns to outperform leading competitor columns and produce less bleed at a higher maximum temperature (370 °C) than competitor columns produce at their lower maximum temperature (360 °C). Less interference from column bleed means more accurate and sensitive detection of edible oil triglycerides—and more certainty in the results you report. In addition, higher temperature stability means consistent performance and longer column lifetimes with fewer column changes. Food testing labs will benefit from the reproducible retention times, robust inertness, stable baselines, and consistent column-to-column performance provided by low-bleed Rxi-65TG columns.


Rugged Long-Life ESI Electrodes Provide OEM-Equivalent Results and Longer Lifetimes

The harsh environment inside an MS source takes its toll on ESI electrodes, and once they corrode, sensitivity drops, runs fail, and productivity halts while the instrument is offline for maintenance. To help labs overcome this obstacle, Restek has developed the Long-Life ESI electrode for Sciex Turbo V sources, which is more rugged than original equipment manufacturer (OEM) electrodes. Testing shows that Long-Life ESI electrodes provide equivalent LC-MS/MS performance to OEM electrodes over significantly longer lifetimes. Because they perform the same and are less susceptible to corrosive conditions in the MS source, Restek Long-Life ESI electrodes ensure more instrument uptime for sample analysis and are a particularly good choice for high-throughput laboratories.
View testing results and learn more today at www.restek.com/LongLifeESI.