Novel Stationary Phase for Comprehensive PFAS Analysis: Ultrashort-Chain (C2, C3), Alternative, and Legacy Compounds

Published By: Restek Corporation

Year of Publication: 2020

Link: https://www.restek.com/Technical-Resources/Technical-Library/Environmental/env_EVFA3197-UNV

Abstract: In this fast, isocratic LC-MS/MS analysis, Raptor Polar X columns provide proper chromatographic retention of TFA and other small, polar ultrashort-chain PFAS as well as short- and long-chain PFAS.

Simple Large Volume Injection Method for Trace-Level Glyphosate in Bottled Drinking Water

Published By: Restek Corporation

Year of Publication: 2020

Link: https://www.restek.com/Technical-Resources/Technical-Library/Environmental/env_EVFA3196-UNV

Abstract: Trace-level detection of glyphosate can be achieved without complex derivatization or ion paining by using direct large volume injection of water on a Raptor Polar X column.

Raptor Polar X: Separate a Wide Variety of Polar Analytes with a Novel Hybrid Stationary Phase

Published By: Restek Corporation

Year of Publication: 2020

Link: https://www.restek.com/Technical-Resources/Technical-Library/General-Interest/gen_GNSS3195-UNV

Abstract: The unique hybrid phase in Raptor Polar X columns balances HILIC and ion exchange retention modes, making it ideal for analyzing a wide range of polar compounds, especially when coupled with mass spectrometry.

Separate a Wide Variety of Polar Analytes with New Raptor Polar X Columns

Analyzing polar compounds using liquid chromatography has historically been a challenge. Poor retention and peak shape, complex mobile phases that may not be MS-friendly, long equilibration times, low sensitivity, and sample derivatization are all complications that reduce lab efficiency and productivity. However, new Raptor Polar X columns reliably retain and efficiently separate a broad range of polar analytes using a unique stationary phase that balances two retention mechanisms: HILIC and ion exchange. This hybrid phase is ideal for analyzing acidic, basic, and neutral polar compounds without time-consuming derivatization or complex ion pairing, and it is especially compatible with mass spectrometry. Easy changes to mobile phase conditions allow analysts to switch between modes and selectively tune retention for the compounds of interest without needing long equilibration times. Simplify the analysis of polar compounds with the resolving power of Raptor Polar X columns from Restek.

Learn more about Raptor Polar X columns at www.restek.com/PolarX

About Raptor SPP LC Columns:
Restek chemists became the first to combine the speed of superficially porous particles (also known as SPP or “core-shell” particles) with the resolution of highly selective USLC technology. This new breed of chromatographic column allows you to more easily achieve peak separation and faster analysis times without expensive UHPLC instrumentation.

Learn more about Raptor LC columns at www.restek.com/raptor

Faster Trace-Level Analysis of Acrylamide in Drinking Water and Food

Now available in two column formats for optimal application-specific performance, Allure Acrylamide LC columns enable you to meet method requirements for food and drinking water quickly and easily. Unique column chemistry reliably separates acrylamide from matrix interferences, and our new format for large volume injection lets you reach sensitivity requirements without time-consuming SPE preconcentration. The new 150 x 3.0 mm column provides an optimized balance of larger capacity and MS-friendly flow rates for large volume injection of drinking water samples while the original 50 x 2.1 mm format delivers faster analysis times and fewer column changes for both food and drinking water when used with SPE sample preparation. Pair with Restek guard columns and analytical reference standards to ensure data quality even down to low ppt levels in sample matrix.

Developed to withstand 100% aqueous mobile phases and elute coextracted matrix components rather than strongly retaining them, Restek Allure Acrylamide guard and analytical columns deliver the same robust performance injection to injection and column to column. Our stringent manufacturing procedures and rigorous quality testing ensure that when it does come time to replace your Allure Acrylamide column, you will get the same great chromatographic performance with the new column that you relied on with the old one.

www.restek.com/acrylamide

LC-MS/MS Analysis of Acrylamide in Drinking Water Using Large Volume Injection

Published By: Restek Corporation

Year of Publication: 2020

Link: https://www.restek.com/Technical-Resources/Technical-Library/Environmental/env_EVAR3184-UNV

Abstract: In this work, we share a method for the analysis of acrylamide in drinking water at low ppt levels using large volume injections directly into an LC-MS/MS.

Tips for Preparing Calibration Curve Standards and Avoiding Sources of Error

Author: Landon Wiest

Published By: Restek Corporation

Year of Publication: 2020

Link: https://www.restek.com/Technical-Resources/Technical-Library/General-Interest/gen_GNAR3169-UNV

Abstract: When preparing calibration curve standards, minimizing sources of error is vital for obtaining accurate quantitative results. This article shares best practices relating to equipment, chemical interactions, and workflow solutions that will help ensure accurate and precise analytical results.

Analysis of Pesticides and Mycotoxins in Cannabis Brownies

Author: Nathaly Reyes and Colton Myers

Published By: Restek Corporation

Year of Publication: 2020

Link: https://www.restek.com/Technical-Resources/Technical-Library/Foods-Flavors-Fragrances/fff_FFAN3149-UNV

Abstract: Government regulations in California require the cannabis industry to test edible products for an extensive list of pesticides and mycotoxins. Here, an effective workflow for this complex analysis was developed in brownies and optimization strategies are detailed in order to provide a starting point for similar matrices. Both LC-MS/MS and GC-MS/MS were employed and excellent results for LOQ, linearity, accuracy, and precision were attained for all the target compounds.

Simplify LC Method Migration with Pro EZLC Method Translator

Scaling down an existing LC method to a smaller column format can speed up run time, increase sample throughput, and reduce solvent use, but only if the LC conditions are also properly adjusted. Pro EZLC method translation software simplifies and streamlines this process by taking manual calculations and time-consuming lab work out of the way. Just input your current column dimensions and method conditions, then specify the dimensions of the new column you want to try; the Pro EZLC method translator will automatically generate new injection volumes and isocratic or gradient program conditions. In addition, the software displays speed gains, solvent savings, retention times, and critical pair resolution values so you can quickly assess results for different column options from your desk and be certain of method performance before starting in the lab.

www.restek.com/ezlc

Featured Pittcon Short Course: Practical Maintenance and Troubleshooting in Gas Chromatography

Jaap de Zeeuw

Jaap de Zeeuw

Date: Tuesday, March 03, 2020

Location: Pittcon 2020, Chicago

Course Description: 

In gas chromatography, 90% of the problems encountered can be traced back to issues in the injection system. In this half-day featured Pittcon short course, Restek’s Jaap de Zeeuw will discuss the purpose and impact of the critical parts used in split and splitless injection, as well as how they affect overall system maintenance schedules. Carrier gas choice and purity, tubing, connections, septa, ferrules, seals, liners, column coupling, column installation, and column maintenance all will be covered in detail. In addition, Jaap will discuss column operation/optimization concepts and explain how to extend GC column lifetime most effectively. At the end, we will explore a series of practical examples together through troubleshooting exercises.

Register now