Author(s): Emanuela Gionfriddo1,2,3, German Augusto Gómez-Ríos4
1. Department of Chemistry and Biochemistry, College of Natural Sciences and Mathematics, The University of Toledo, 2. School of Green Chemistry and Engineering, The University of Toledo, 3. Dr. Nina McClelland Laboratory for Water Chemistry and Environmental Analysis, The University of Toledo, 4. Restek Corporation
Published By: The Journal of Mass Spectrometry
Year of Publication: 2020
Abstract: Because of the complexity and diversity of food matrices, their chemical analysis often entails several analytical challenges to attain accurate and reliable results, especially for multiresidue analysis and ultratrace quantification. Nonetheless, microextraction technology, such as solid‐phase microextraction (SPME), has revolutionized the concept of sample preparation for complex matrices because of its nonexhaustive, yet quantitative extraction approach and its amenability to coupling to multiple analytical platforms. In recent years, microextraction devices directly interfaced with mass spectrometry (MS) have redefined the analytical workflow by providing faster screening and quantitative methods for complex matrices. This review will discuss the latest developments in the field of food analysis by means of microextraction approaches directly coupled to MS. One key feature that differentiates SPME‐MS approaches from other ambient MS techniques is the use of matrix compatible extraction phases that prevent biofouling, which could drastically affect the ionization process and are still capable of selective extraction of the targeted analytes from the food matrix. Furthermore, the review examines the most significant applications of SPME‐MS for various ionization techniques such as direct analysis in real time, dielectric barrier desorption ionization, and some unique SPME geometries, for example, transmission mode SPME and coated blade spray, that facilitate the interface to MS instrumentation.
Restek coated blade spray (CBS) technology accelerates rapid-screening analyses to a new level of efficiency and speed. Based on strong sample extraction and ionization foundations, CBS technology bridges the gap between sample preparation and MS/MS analysis by providing a product that can go directly from the sample to the mass spectrometer. Rapid-screening workflows that once required complex, distinct, sample preparation methods and chromatographic separations are reduced to a few simple steps. And your most powerful analytical tool—chromatography—is reserved for only select samples that need additional analysis.
As a technology on the cutting edge of commercialization, coated blade spray will continue to evolve, but for laboratories interested in evaluating how coated blade spray can revolutionize their analytical workflows, Restek is proud to present the first in a long line of direct-to-MS products to come: the coated CB-HLB blade. A precision-made, stainless-steel blade supports a specially prepared coating of hydrophilic-lipophilic balanced (HLB) sorbent, making the CB-HLB a finely tuned sample preparation product.
Ionization interfaces and a completely commercialized solution are under development and coming soon, but if your lab performs screening analyses, or if you are interested in finding out more about coated blade spray technology from Restek, sign up at
Once you do, we will begin sending you periodic updates on new CBS developments and may also contact you to further discuss coated blade spray or to answer any questions you may have.
Concern over persistent organic pollutants, or POPs, only continues to grow. Analytically, these compounds also present a lot of challenges requiring consistency while achieving ultralow-level sensitivity. On Thursday, September 24, Restek’s Chris Rattray is presenting the webinar “Persistent Ongoing Perfection: Optimization of a GC-MS/MS Method for the Analysis of POPs Plus an Alternate Approach Utilizing High-resolution Mass Spectrometry” along with a team of industry experts as part of Thermo Fisher Scientific’s Environmental Webinar Week.
Learn more and register!
Mycotoxins are toxic secondary metabolite compounds produced by mold or fungi. Due to the toxicity of certain mycotoxins that can be found on cannabis plants, there is a growing need to detect their presence in both raw plant materials and finished cannabis products.
Restek now offers two new mycotoxin certified reference materials (CRMs): Ochratoxin A (cat.# 34122) and Aflatoxins (B1, B2, G1, G2) (cat.# 34121).
These new reference standards enable labs to expand their cannabis analyses with a Restek solution when coupled with our high-throughput Raptor ARC-18 (cat.# 9314A12) LC column.
For mycotoxins and other reference standards, Raptor LC columns, sample preparation products, and the expert consultation your cannabis lab needs, turn to www.restek.com/cannabis
Author(s): Sharon A. Lupo, Randall L. Romesberg, Xiaoning Lu
Published By: Journal of Chromatography A
Year of Publication: 2020
Abstract: An automated inline sample preparation (ILSP) method has been developed for pesticide residue analysis in spinach by LC-MS/MS. Chlorophyll pigments and other matrix constituents were removed from the sample extract using a UHPLC system equipped with an auxiliary pump, 6-port high pressure switching valve, and dual-directional ILSP cartridge containing bonded silica. The new procedure was evaluated as an entirely separate workflow using a simple solid-liquid extraction and as part of a cleanup strategy in conjunction with QuEChERS. Accuracy and precision experiments were conducted in spinach at two concentration levels (n=6). Of the 63 pesticides tested, 86% (0.005 mg/kg) and 100% (0.05 mg/kg) displayed average recoveries within 70-120% and RSD values ≤20% for the ILSP method. In addition, low to moderate matrix effects (<50%) were calculated for 95% of the analytes. Overall performance of the proposed method was found to be better or comparable to a traditional QuEChERS procedure utilizing AOAC formulated salts and dSPE sorbents, while significantly reducing the amount of pigments reaching the MS source. The ILSP workflow is a simpler procedure with fewer steps that require less time than traditional extraction and cleanup techniques.
Cannabidivarinic Acid (CBDVA) is a non-psychoactive cannabinoid reported to have anti-inflammatory properties. While its medicinal benefits are still being researched, quantifying this compound in cannabis samples is crucial for obtaining a true measurement of potency and chemovar identification. Restek now offers a new CBDVA certified reference material (CRM) to help labs advance their analysis of cannabis.
Restek’s new CBDVA standard is ideal for creating multipoint (5-point minimum suggested) calibration curves for LC analysis. With verified composition and stability, this prepared stock product eliminates the need for in-house standards preparation for greater convenience and lower labor requirements. As it’s excluded from U.S. DEA Controlled Substances Act (CSA) regulatory controls, no customer permits or licensing required to purchase within the U.S., and because it’s manufactured and QC tested in Restek’s ISO-accredited labs, this new CBDVA standard qualifies as a certified reference material (CRM) that satisfies your ISO requirements.
For CBDVA and other reference standards, Raptor LC columns, sample preparation products, and the expert consultation your cannabis lab needs, turn to www.restek.com/cannabis
Restek has expanded its line of Resprep solid phase extraction (SPE) products with the addition of polymeric sorbents, available in both cartridges for high loading capacity and 96-well plates for high throughput and automation.
Because Resprep polymeric SPE products feature a silica-free, bonded polymeric material, there are no unwanted secondary silica interactions, even with basic compounds. Their high surface area means a higher loading capacity compared to silica sorbents, and their stability over a wide pH range (0–14) means they won’t hydrolyze under extreme conditions. New Resprep polymeric SPE cartridges and 96-well plates are water-wettable, allowing for streamlined conditioning and equilibration steps to drastically reduce solvent usage and sample prep time. And they are not flow-rate dependent, so they maintain retention and capacity after conditioning, even if dried out from vacuum or positive pressure flows.
Resprep polymeric SPE products offer five distinct packings to choose from. Find them all, and the entire line of Resprep sample preparation products, at www.restek.com/resprep
We have uploaded our RAFA 2019 presentations to our website for your review and study. Feel free to contact the authors directly with any questions you may have.
We have uploaded our SOFT 2019 presentations to our website for your review and study. Feel free to contact the authors directly with any questions you may have.
Cannabicyclol (CBL) is formed through the photodegradation of cannabichromene (CBC). Levels are typically quite low, and the medicinal benefits of CBL are still being researched, but quantifying this compound in cannabis samples is crucial for obtaining a more-complete cannabinoids profile. Restek has just released a new CBL certified reference material (CRM) to further aid labs in the comprehensive characterization of cannabis: www.restek.com/catalog/view/54615
Restek’s new CBL standard is ideal for creating multipoint (5-point minimum suggested) calibration curves for LC analysis. With verified composition and stability, this prepared stock product eliminates the need for in-house standards preparation for greater convenience and lower labor requirements. As a U.S. DEA-exempted formulation, no additional customer permits or licensing are required to purchase within the U.S., and because it’s manufactured and QC tested in Restek’s ISO-accredited labs, this new CBL standard qualifies as a certified reference material (CRM) that satisfies your ISO requirements.
For CBL and other reference standards, Raptor LC columns, sample preparation products, and the expert consultation your cannabis lab needs, turn to www.restek.com/cannabis