Scaling down an existing LC method to a smaller column format can speed up run time, increase sample throughput, and reduce solvent use, but only if the LC conditions are also properly adjusted. Pro EZLC method translation software simplifies and streamlines this process by taking manual calculations and time-consuming lab work out of the way. Just input your current column dimensions and method conditions, then specify the dimensions of the new column you want to try; the Pro EZLC method translator will automatically generate new injection volumes and isocratic or gradient program conditions. In addition, the software displays speed gains, solvent savings, retention times, and critical pair resolution values so you can quickly assess results for different column options from your desk and be certain of method performance before starting in the lab.
Restek has expanded its line of Resprep solid phase extraction (SPE) products with the addition of polymeric sorbents, available in both cartridges for high loading capacity and 96-well plates for high throughput and automation.
Because Resprep polymeric SPE products feature a silica-free, bonded polymeric material, there are no unwanted secondary silica interactions, even with basic compounds. Their high surface area means a higher loading capacity compared to silica sorbents, and their stability over a wide pH range (0–14) means they won’t hydrolyze under extreme conditions. New Resprep polymeric SPE cartridges and 96-well plates are water-wettable, allowing for streamlined conditioning and equilibration steps to drastically reduce solvent usage and sample prep time. And they are not flow-rate dependent, so they maintain retention and capacity after conditioning, even if dried out from vacuum or positive pressure flows.
Resprep polymeric SPE products offer five distinct packings to choose from. Find them all, and the entire line of Resprep sample preparation products, at www.restek.com/resprep
Simultaneously remove phospholipids and proteins in a single, simple procedure with new Resprep PLR (phospholipid removal) SPE products. Whole blood, serum, and plasma all contain proteins and phospholipids that can interfere with target analytes and hasten the need for instrument maintenance. It’s important to remove them from samples prior to analysis to avoid signal suppression, and Resprep PLR SPE cartridges or 96-well plates make this an easy task by combining protein precipitation and phospholipid removal in one sample preparation process. No analyte-specific method development is required because the same procedure can be used for samples containing acids, bases, or neutral compounds. In addition, effective removal of phospholipids and proteins from sample extracts reduces contamination, minimizing the frequency of instrument maintenance.
Cross-contamination of samples run in series through typical SPE vacuum manifolds is a common problem for sample preparation labs. New Resprep Quick-Replace vacuum manifolds eliminate this risk because they contain a disposable valve liner that can be quickly and easily replaced between samples, ensuring a clean flow path for every extraction. In addition, the screw-type valve design provides precise, independent flow control of each SPE port. Resprep Quick-Replace vacuum manifolds are compatible with any standard male luer SPE cartridge and are available in 12- and 24-port models. The easily configurable rack system supports a wide variety of collection vessels and can be customized to accommodate vessel size.
The porous graphitized carbon (PGC) columns typically used for acrylamide analysis by method EN 16618:2015 provide adequate retention of acrylamide and sufficient separation from matrix components, but they are subject to long run times and short column lifetimes. Switching to an Allure Acrylamide LC column is a better alternative: an Allure Acrylamide column used with a deuterated internal standard provides faster total analysis times and meets system suitability requirements longer.
Allure Acrylamide columns meet the EN method 1.7-minute retention time requirement with good separation from matrix components and a much shorter equilibration time. A per analysis savings of 2.5 minutes allows more samples to be analyzed per shift, significantly increasing lab output. In addition to faster run times, testing showed that the Allure Acrylamide column passed the 1.7-minute system suitability requirement for over twice as many injections as a typical PGC column.
Developed to withstand 100% aqueous mobile phases and elute coextracted matrix components rather than strongly retaining them, Restek Allure Acrylamide guard and analytical columns deliver the same robust performance injection to injection and column to column, making them a more reliable choice for acrylamide analysis, even in difficult food matrices like potato chips or coffee.
Cannabicyclol (CBL) is formed through the photodegradation of cannabichromene (CBC). Levels are typically quite low, and the medicinal benefits of CBL are still being researched, but quantifying this compound in cannabis samples is crucial for obtaining a more-complete cannabinoids profile. Restek has just released a new CBL certified reference material (CRM) to further aid labs in the comprehensive characterization of cannabis: www.restek.com/catalog/view/54615
Restek’s new CBL standard is ideal for creating multipoint (5-point minimum suggested) calibration curves for LC analysis. With verified composition and stability, this prepared stock product eliminates the need for in-house standards preparation for greater convenience and lower labor requirements. As a U.S. DEA-exempted formulation, no additional customer permits or licensing are required to purchase within the U.S., and because it’s manufactured and QC tested in Restek’s ISO-accredited labs, this new CBL standard qualifies as a certified reference material (CRM) that satisfies your ISO requirements.
For CBL and other reference standards, Raptor LC columns, sample preparation products, and the expert consultation your cannabis lab needs, turn to www.restek.com/cannabis
With best-in-class thermal stability, new Rxi-65TG columns from Restek are the top choice for accurate, reliable analysis of triglycerides (acylglycerols) in edible oils. Novel chemistry and cutting-edge manufacturing allow Rxi-65TG columns to outperform leading competitor columns and produce less bleed at a higher maximum temperature (370 °C) than competitor columns produce at their lower maximum temperature (360 °C). Less interference from column bleed means more accurate and sensitive detection of edible oil triglycerides—and more certainty in the results you report. In addition, higher temperature stability means consistent performance and longer column lifetimes with fewer column changes. Food testing labs will benefit from the reproducible retention times, robust inertness, stable baselines, and consistent column-to-column performance provided by low-bleed Rxi-65TG columns.
The harsh environment inside an MS source takes its toll on ESI electrodes, and once they corrode, sensitivity drops, runs fail, and productivity halts while the instrument is offline for maintenance. To help labs overcome this obstacle, Restek has developed the Long-Life ESI electrode for Sciex Turbo V sources, which is more rugged than original equipment manufacturer (OEM) electrodes. Testing shows that Long-Life ESI electrodes provide equivalent LC-MS/MS performance to OEM electrodes over significantly longer lifetimes. Because they perform the same and are less susceptible to corrosive conditions in the MS source, Restek Long-Life ESI electrodes ensure more instrument uptime for sample analysis and are a particularly good choice for high-throughput laboratories.
View testing results and learn more today at www.restek.com/LongLifeESI.
Cannabis strains with high levels of cannabidiol (CBD) typically have higher levels of cannabidivarin (CBDV) as well, but accurate quantitation of both compounds for potency and strain ID is a must, especially as interest in using CBDV as an anticonvulsant and anti-nausea treatment continues to rise. Restek now offers a stand-alone CBDV reference standard—in addition to CBD and a host of other compounds—to help labs with the comprehensive analysis of cannabis products.
Restek’s CBDV standard is ideal for creating multipoint (5-point minimum suggested) calibration curves for LC-MS/MS. With verified composition and stability, this prepared stock product eliminates the need for in-house standards preparation for greater convenience and lower labor requirements. As a U.S. DEA-exempted formulation, no additional customer permits or licensing are required to purchase within the U.S., and because it’s manufactured and QC tested in Restek’s ISO-accredited labs, this new CBDV standard qualifies as a certified reference material (CRM) that satisfies your ISO requirements.
For CBDV, CBD, and other cannabis reference standards, along with the expertise and consumables your lab needs, turn to www.restek.com/cannabis
New and improved, Restek’s electronic leak detector lets you pinpoint small gas leaks quickly and accurately before they cause bigger problems. Now, there’s no more waiting for a full charge—the unit can be operated during charging or used up to 12 hours between charges. This compact, handheld instrument detects a broad range of laboratory gases and indicates leak severity with both an LED display and audible tone. In addition, it comes with a flexible charging kit that includes both a universal AC power adaptor and a USB charging cable so you can charge anywhere, anytime. Using a Restek electronic leak detector to ensure all critical seals in the GC system are leak free is an easy way to prevent costly downtime, waste, and damage.