Analyzing polar compounds using liquid chromatography has historically been a challenge. Poor retention and peak shape, complex mobile phases that may not be MS-friendly, long equilibration times, low sensitivity, and sample derivatization are all complications that reduce lab efficiency and productivity. However, new Raptor Polar X columns reliably retain and efficiently separate a broad range of polar analytes using a unique stationary phase that balances two retention mechanisms: HILIC and ion exchange. This hybrid phase is ideal for analyzing acidic, basic, and neutral polar compounds without time-consuming derivatization or complex ion pairing, and it is especially compatible with mass spectrometry. Easy changes to mobile phase conditions allow analysts to switch between modes and selectively tune retention for the compounds of interest without needing long equilibration times. Simplify the analysis of polar compounds with the resolving power of Raptor Polar X columns from Restek.
Learn more about Raptor Polar X columns at www.restek.com/PolarX
About Raptor SPP LC Columns:
Restek chemists became the first to combine the speed of superficially porous particles (also known as SPP or “core-shell” particles) with the resolution of highly selective USLC technology. This new breed of chromatographic column allows you to more easily achieve peak separation and faster analysis times without expensive UHPLC instrumentation.
Learn more about Raptor LC columns at www.restek.com/raptor
Cannabidivarinic Acid (CBDVA) is a non-psychoactive cannabinoid reported to have anti-inflammatory properties. While its medicinal benefits are still being researched, quantifying this compound in cannabis samples is crucial for obtaining a true measurement of potency and chemovar identification. Restek now offers a new CBDVA certified reference material (CRM) to help labs advance their analysis of cannabis.
Restek’s new CBDVA standard is ideal for creating multipoint (5-point minimum suggested) calibration curves for LC analysis. With verified composition and stability, this prepared stock product eliminates the need for in-house standards preparation for greater convenience and lower labor requirements. As it’s excluded from U.S. DEA Controlled Substances Act (CSA) regulatory controls, no customer permits or licensing required to purchase within the U.S., and because it’s manufactured and QC tested in Restek’s ISO-accredited labs, this new CBDVA standard qualifies as a certified reference material (CRM) that satisfies your ISO requirements.
For CBDVA and other reference standards, Raptor LC columns, sample preparation products, and the expert consultation your cannabis lab needs, turn to www.restek.com/cannabis
Now available in two column formats for optimal application-specific performance, Allure Acrylamide LC columns enable you to meet method requirements for food and drinking water quickly and easily. Unique column chemistry reliably separates acrylamide from matrix interferences, and our new format for large volume injection lets you reach sensitivity requirements without time-consuming SPE preconcentration. The new 150 x 3.0 mm column provides an optimized balance of larger capacity and MS-friendly flow rates for large volume injection of drinking water samples while the original 50 x 2.1 mm format delivers faster analysis times and fewer column changes for both food and drinking water when used with SPE sample preparation. Pair with Restek guard columns and analytical reference standards to ensure data quality even down to low ppt levels in sample matrix.
Developed to withstand 100% aqueous mobile phases and elute coextracted matrix components rather than strongly retaining them, Restek Allure Acrylamide guard and analytical columns deliver the same robust performance injection to injection and column to column. Our stringent manufacturing procedures and rigorous quality testing ensure that when it does come time to replace your Allure Acrylamide column, you will get the same great chromatographic performance with the new column that you relied on with the old one.
Scaling down an existing LC method to a smaller column format can speed up run time, increase sample throughput, and reduce solvent use, but only if the LC conditions are also properly adjusted. Pro EZLC method translation software simplifies and streamlines this process by taking manual calculations and time-consuming lab work out of the way. Just input your current column dimensions and method conditions, then specify the dimensions of the new column you want to try; the Pro EZLC method translator will automatically generate new injection volumes and isocratic or gradient program conditions. In addition, the software displays speed gains, solvent savings, retention times, and critical pair resolution values so you can quickly assess results for different column options from your desk and be certain of method performance before starting in the lab.
Restek has expanded its line of Resprep solid phase extraction (SPE) products with the addition of polymeric sorbents, available in both cartridges for high loading capacity and 96-well plates for high throughput and automation.
Because Resprep polymeric SPE products feature a silica-free, bonded polymeric material, there are no unwanted secondary silica interactions, even with basic compounds. Their high surface area means a higher loading capacity compared to silica sorbents, and their stability over a wide pH range (0–14) means they won’t hydrolyze under extreme conditions. New Resprep polymeric SPE cartridges and 96-well plates are water-wettable, allowing for streamlined conditioning and equilibration steps to drastically reduce solvent usage and sample prep time. And they are not flow-rate dependent, so they maintain retention and capacity after conditioning, even if dried out from vacuum or positive pressure flows.
Resprep polymeric SPE products offer five distinct packings to choose from. Find them all, and the entire line of Resprep sample preparation products, at www.restek.com/resprep
Simultaneously remove phospholipids and proteins in a single, simple procedure with new Resprep PLR (phospholipid removal) SPE products. Whole blood, serum, and plasma all contain proteins and phospholipids that can interfere with target analytes and hasten the need for instrument maintenance. It’s important to remove them from samples prior to analysis to avoid signal suppression, and Resprep PLR SPE cartridges or 96-well plates make this an easy task by combining protein precipitation and phospholipid removal in one sample preparation process. No analyte-specific method development is required because the same procedure can be used for samples containing acids, bases, or neutral compounds. In addition, effective removal of phospholipids and proteins from sample extracts reduces contamination, minimizing the frequency of instrument maintenance.
Cross-contamination of samples run in series through typical SPE vacuum manifolds is a common problem for sample preparation labs. New Resprep Quick-Replace vacuum manifolds eliminate this risk because they contain a disposable valve liner that can be quickly and easily replaced between samples, ensuring a clean flow path for every extraction. In addition, the screw-type valve design provides precise, independent flow control of each SPE port. Resprep Quick-Replace vacuum manifolds are compatible with any standard male luer SPE cartridge and are available in 12- and 24-port models. The easily configurable rack system supports a wide variety of collection vessels and can be customized to accommodate vessel size.
The porous graphitized carbon (PGC) columns typically used for acrylamide analysis by method EN 16618:2015 provide adequate retention of acrylamide and sufficient separation from matrix components, but they are subject to long run times and short column lifetimes. Switching to an Allure Acrylamide LC column is a better alternative: an Allure Acrylamide column used with a deuterated internal standard provides faster total analysis times and meets system suitability requirements longer.
Allure Acrylamide columns meet the EN method 1.7-minute retention time requirement with good separation from matrix components and a much shorter equilibration time. A per analysis savings of 2.5 minutes allows more samples to be analyzed per shift, significantly increasing lab output. In addition to faster run times, testing showed that the Allure Acrylamide column passed the 1.7-minute system suitability requirement for over twice as many injections as a typical PGC column.
Developed to withstand 100% aqueous mobile phases and elute coextracted matrix components rather than strongly retaining them, Restek Allure Acrylamide guard and analytical columns deliver the same robust performance injection to injection and column to column, making them a more reliable choice for acrylamide analysis, even in difficult food matrices like potato chips or coffee.
Cannabicyclol (CBL) is formed through the photodegradation of cannabichromene (CBC). Levels are typically quite low, and the medicinal benefits of CBL are still being researched, but quantifying this compound in cannabis samples is crucial for obtaining a more-complete cannabinoids profile. Restek has just released a new CBL certified reference material (CRM) to further aid labs in the comprehensive characterization of cannabis: www.restek.com/catalog/view/54615
Restek’s new CBL standard is ideal for creating multipoint (5-point minimum suggested) calibration curves for LC analysis. With verified composition and stability, this prepared stock product eliminates the need for in-house standards preparation for greater convenience and lower labor requirements. As a U.S. DEA-exempted formulation, no additional customer permits or licensing are required to purchase within the U.S., and because it’s manufactured and QC tested in Restek’s ISO-accredited labs, this new CBL standard qualifies as a certified reference material (CRM) that satisfies your ISO requirements.
For CBL and other reference standards, Raptor LC columns, sample preparation products, and the expert consultation your cannabis lab needs, turn to www.restek.com/cannabis
With best-in-class thermal stability, new Rxi-65TG columns from Restek are the top choice for accurate, reliable analysis of triglycerides (acylglycerols) in edible oils. Novel chemistry and cutting-edge manufacturing allow Rxi-65TG columns to outperform leading competitor columns and produce less bleed at a higher maximum temperature (370 °C) than competitor columns produce at their lower maximum temperature (360 °C). Less interference from column bleed means more accurate and sensitive detection of edible oil triglycerides—and more certainty in the results you report. In addition, higher temperature stability means consistent performance and longer column lifetimes with fewer column changes. Food testing labs will benefit from the reproducible retention times, robust inertness, stable baselines, and consistent column-to-column performance provided by low-bleed Rxi-65TG columns.