DiatoSorb-W Diatomaceous Earth: Great Chromatography Millions of Years in the Making

diatosorb_primageDiatoSorb-W diatomaceous earth is a new alternative for analysts who depend on diatomaceous earth columns and struggle to find consistent product quality and reliable availability. Restek has developed DiatoSorb-W as a high-performing replacement for the diatomaceous earth that was previously used in their standard and Silcoport W GC packed columns lines. DiatoSorb-W solid support is available in acid-washed (WAW), non-acid washed (WNAW), and high-performance (WHP) varieties, and all DiatoSorb-W solid support undergoes rigorous quality testing to ensure high purity and dependable performance. Virtually unlimited in supply—but only available from Restek—new DiatoSorb-W offers a reliable alternative for packed column users. Whether you need packed columns or loose packing materials, DiatoSorb-W products from Restek are always available and consistent in quality.

Learn more at www.restek.com/DiatoSorb-W

Restek’s Mike Shuey Earns 2016 ESOP Outstanding Chapter Officer Award

shuey_award_twitter_dknMike Shuey, Restek’s International Customer Service Supervisor, received the 2016 ESOP Outstanding Chapter Officer Award at The ESOP Association (TEA) Awards Banquet earlier this year. Mike was selected from a field of over 100 ESOP chapter officers for his significant contributions to TEA. As PA/DE chapter officer, Mike leads the legislative advocacy committee, manages the chapter advocacy web site, and coordinates ESOP education not only for other chapter members but also for members of Congress. He is also responsible for generating legislative officer goals for the year and seeing they are met to the best of his ability. Mike takes great pride in being a part of legislative efforts to support and educate our representatives to keep our ESOP benefits in place and is proud of Restek’s grass roots efforts to show the nation that our ESOP model works and is a positive business strategy for the success of our country.

Restek’s Jaap de Zeeuw to Present at Food Science 2016 in Singapore

Jaap de Zeeuw

Jaap de Zeeuw

On November 16–17, Separation Science will again host Food Science 2016 in Singapore. This world-class international conference and exhibition covers key food analysis topics including safety, traceability, authenticity, and ingredients analysis. Restek invites you to attend the show at a special discounted rate. In addition to Jaap de Zeeuw’s presentation, you may attend over 30 other talks on food analysis, quality, and safety.


While you’re the Food 2016 conference, be sure to stop by Booth 112 and visit your local Restek representative, Lab Science Solution. There will be games, giveaways, and information on Restek products and solutions!


Oral Presentation

Wednesday, November 16
Reduce Analysis Time and Optimize GC Separations in Food Analysis, while Maintaining the Same Peak Elution Order: A New Free Software Tool in GC—the EZGC Method Translator

Jaap de Zeeuw, Restek Corporation

In gas chromatography, there is often a need to optimize separations using different column dimensions, linear gas velocities, detectors, or carrier gases. If you want to get the same peak elution order (same chromatogram), you must make sure that the elution temperatures of the components are kept the same. This can only be done by using a different oven temperature program. To calculate this program, there are free calculation programs available on the web. In this course, we will discuss the details of method conversion using Restek’s EZGC method translator so that you get the same chromatograms with the new method. The course will also cover how existing separations can easily be checked and corrected for optimal separation.


Poster Presentations

Shoot-and-Dilute GC-MS/MS: Use of Split Injection for Pesticide Residue Screening to Prolong GC Inlet Liner and Column Performance

 Jaap de Zeeuw, Julie Kowalski, and Jack Cochran, Restek Corporation

 Shoot-and-dilute GC-MS/MS uses split injection for GC paired with a very sensitive triple quadrupole mass spectrometer. Split injection can alleviate matrix-related issues that occur at the GC inlet and column. There are well-known problems associated with splitless injection of dirty samples, most notably compound degradation and drastic response changes. This can occur very quickly with real samples, sometimes with a single injection of a particularly dirty sample. Inlet and column maintenance are needed to restore instrument performance resulting in instrument downtime. An easy way to mitigate these problems is to use split injection when possible. That is, if the limit of detection and limit of quantitation requirements are achievable using split injections at ratios of 10:1 or greater. Increased flow through the GC inlet with split injection minimizes residence time inside the inlet liner. This decreases compound degradation and maintains acceptable data quality longer, especially during dirty sample analyses.

In addition to the benefits described above, the GC oven start temperature can be higher, thus reducing overall analysis time as well as the time needed to re-equilibrate the oven. Also, split injection of the common QuEChERS solvent, acetonitrile, is easily accommodated on a nonpolar 5-type GC column, allowing symmetrical peak shapes of early eluting compounds like methamidophos, dichlorvos, and acephate. This eliminates the need for extensive initial oven temperature optimization or time-consuming solvent exchange.

Shoot-and-dilute GC-MS/MS was tested for multiclass pesticides and compared with a splitless injection method. Split injection and the initial GC oven temperature parameters were optimized. Viability of split injection based on detectability of over 200 analytes was determined in QuEChERS-prepared green bell pepper, celery, and orange. Green bell pepper cleanup using dispersive SPE without graphitized carbon black sorbent was less than ideal with a relatively high level of chlorophyll remaining in the sample, which severely stressed GC inlet performance for splitless work while the split method was much more robust. Ruggedness of split and splitless methods was evaluated by comparing average response factors and corresponding %RSDs and by showing chromatographic performance differences, especially for compounds that show degradation products.


Wool Packing or No Wool Packing in a Splitless GC Inlet Liner–Which is better for Pesticide Analysis? A Case Study with a QuEChERS Strawberry Extract

 Jaap de Zeeuw and Jack Cochran, Restek Corporation

 Pesticide chemists usually avoid glass wool packing for splitless injection GC because sometimes lower responses are seen for active or thermally unstable pesticides (e.g., carbaryl, DDT, iprodione, etc.) However, properly deactivated quartz wool is not only a valuable protector of the GC column from nonvolatile “dirt,” but it also offers increased responses for pesticides because it stops them from hitting the bottom seal in a typical GC inlet where they can be lost or degraded.

This study compared GC-MS response factors for a group of pesticides ranging in volatility and class (organochlorine, organonitrogen, organophosphorus, carbamate, and pyrethroid) that were fortified into a QuEChERS strawberry extract. The extract was then splitless injected into single taper liners with wool and without wool. The initial response factors were higher for the wool-packed inlet liner versus the liner without wool. After 60 analyses of strawberry extract on each liner, some of the more difficult pesticides showed lower response factors on both liner types, but overall the wool-packed liner still showed higher response factors than the liner with no wool.

A final part of the study compared response factors for deactivated quartz wool liner packing to deactivated borosilicate wool liner packing. Deactivated quartz wool was significantly better for analyzing dimethoate, chlorothalonil, carbaryl, methiocarb, dicofol, and deltamethrin.






Forensic Characterization of Drug Exposure from Skeletal Remains (Featuring Raptor Biphenyl LC columns)

Author: James Watterson

Published By: SelectScience

Year of Publication: 2016

Link: http://www.selectscience.net/editorial-articles/forensic-characterization-of-drug-exposure-from-skeletal-remains/?artID=42183#.WAeNLLeGnOE.twitter


Dr. James Watterson, Associate Professor, Department of Forensic Science at Laurentian University in Ontario, told SelectScience about his forensic toxicology research and the technologies he utilizes in his work. Dr. Watterson’s primary interest is the characterization of drug and metabolite disposition in skeletal remains, and he has employed a variety of chromatography and mass spectrometry-based analyses. The team is now investigating the probative power of quantitative drug-metabolite relationships in bone using LC-MS techniques, which offer greater sensitivity and selectivity for analyses of a number of different metabolites. As Dr. Watterson explains, “Now that we have switched to UPLC-qTOF-MS as our primary analytical approach, the advantages are absolute: substantially reduced sample preparation requirements, vastly improved sensitivity and selectivity, and a much greater of analytes that may be assayed.” The choice of LC column for such analyses is also of critical importance, the correct column chemistry is essential for efficient separation. For example, the team used Raptor Biphenyl columns, with long column geometry and small particle/fused-core stationary phase, to maximize resolution of polar metabolites of phenothiazine drugs, using UPLC-PDA. This approach was critical in the characterization of these metabolites and of the phenothiazine oxidation products, which are produced during sample preparation. Dr. Watterson comments that, “the biphenyl column has been very helpful in resolving those compounds too, as we applied this method to UPLC-qTOF-MS.” Dr. Watterson advises choosing reagents and columns carefully, as background impurities are much more visible using current technologies.

Visit Us at Gulf Coast 2016 and Attend Our Restek/Shimadzu Petrochem GC Applications Workshop

Restek’s Jan Pijpelink and Shimadzu’s Jeff Werner will provide a workshop overview of various petroleum applications using gas chromatography at the 2016 Gulf Coast Conference on Wednesday, October 12. Covered topics include simulated distillation (SimDist), refinery gas analysis, detailed hydrocarbon analysis (DHA), various gasoline applications and light hydrocarbon analysis as well as a new analysis for methanol in natural gas and LPG. Shimadzu gas chromatographs in combination with dedicated Restek columns are designed, tested, and guaranteed for the applications you run.

Wednesday, October 12, 2016 – 8:30 – 11:30 a.m., Room 381 B
Restek/Shimadzu Petrochem GC Applications Workshop

Presenters: Jan Pijpelink1; Jeff Werner2

  1. Restek Corporation, 2. Shimadzu Corporation

Register at:  https://events.r20.constantcontact.com/register/eventReg?oeidk=a07ed4742u31bca294a&oseq=&c=&ch

In addition to attending our workshop, you’ll also want to stop by booth 504 and be sure to catch our presentations as well. You can find abstracts and times here: www.gulfcoastconference.com/abstracts.cfm?year=2016

For more information about the 2016 Gulf Coast Conference, visit www.gulfcoastconference.com/