Simplify the Switch to HILIC with Raptor HILIC-Si Columns

Restek is bringing the speed and reliability of Raptor LC columns into the HILIC realm with the addition of the Raptor HILIC-Si column. HILIC is an increasingly popular alternative to reversed-phase LC for challenging polar analytes because it provides better retention of water-soluble compounds that are separated by polar differences. The Raptor HILIC-Si column simplifies the switch to HILIC because it delivers rugged Raptor performance, provides SPP column speed for faster analyses than traditional FPP silica columns, retains polar compounds without ion-pairing reagents, and is fully reliable, efficient, and selective with LC-MS compatible mobile phases.

Restek chemists became the first to combine the speed of superficially porous particles (also known as SPP or “core-shell” particles) with the resolution of highly selective USLC technology. This new breed of chromatographic column allows you to more easily achieve peak separation and faster analysis times without expensive UHPLC instrumentation. Learn more about Raptor LC columns at

Rugged, reliable Raptor HILIC-Si columns make the analysis of polar compounds fast, easy, and MS friendly!

Restek Helps Make Fourth of July Fun for the Family

Restek employee-owners serve cake at the Central PA 4thFest

Each year, dozens of employees volunteer to serve more than 1,440 pieces of American Flag cake

It starts with 240 pounds of cake mix and 145 pounds of icing—those are the first ingredients to a successful Fourth of July.

Since 2001, employees at Restek have served up an American flag cake, 12-feet long and nearly six-feet wide, to attendees of Central PA 4thFest—a daylong, family-oriented, patriotic birthday party held July 4 on the campus of The Pennsylvania State University.

Each year, the GIANT store on North Atherton Street donates the cake to the festival after spending eight hours baking and decorating it. It takes about eight Restek volunteers to load the cake and deliver it to the event where it is cut into more than 1,440 slices and served by 20 more Restek volunteers.

“It’s a family affair—many Restek employees bring their families to help,” Bradley Pike, Digital Marketing Manager at Restek, said.

Pike, along with Pam Decker, Senior Graphic Designer at Restek, have been part of the volunteer effort from Restek for more than a decade and both serve on the festival’s board of directors and as committee chairs.

“When Restek first started getting involved, it was apparent the employees really enjoyed it so we wanted to do more,” Decker said. “Now, we have dozens of volunteers helping each year.”

Karl Libhart, executive director of 4thFest, said organizations like Restek play a vital role in putting on the event, which the Travel Channel called “one of the best fireworks displays in the world.”

“Fourth Fest is 100 percent run by volunteers and Restek has been a huge help in ensuring the event is a success every year,” he said.

This year’s event will welcome the return of roller derby in the parking lot, chainsaw carvers, a BMX stunt show, and music from up-and-coming country artist Adley Stump.

Fourth Fest leadership doesn’t stop with Pike and Decker; several fellow Restek employees hold leadership roles in the organization, too. Wendy Krall, Brian Yangula, Titus Morehead, and AJ Saclyn all help to coordinate key parts of the event from the flag cake to the kids’ crafts to the merchandising.

“It’s great to have fresh people bring in fresh ideas each year,” Pike said. “We have a leadership tenet here, so stepping up is part of Restek’s DNA.”

Preparations for next year will begin almost as soon as the event ends.

Pike said one of the most rewarding experiences of putting on the event was the additional level of preparation when country music star Craig Morgan performed in 2015.

“It was a whole other level of preparation that year,” he said. “But, I remember watching the concert with several other 4thFest volunteers and thinking—wow, the community did this.”

To learn more about Restek Corporation, visit

Restek Graduates its 23rd Leadership Centre County Enrollee

Becky Wittrig, Restek’s Director of Global Sales,
and recent LCC graduate

Congratulations to Becky Wittrig, Restek’s Director of Global Sales, who recently graduated with the 25th anniversary class of Leadership Centre County (LCC). Becky is Restek’s 23rd employee to graduate from the program.

LCC is a community leadership development program that has been active in Centre County since 1992, but is one of more than 1,000 similar programs across the country. With the graduation of this year’s class, LCC has a total of 877 alumni donating more than 98,000 hours of their time per year at a value exceeding $2.3 million.

Restek LCC graduates hold leadership or key volunteer positions in a variety of nonprofit organizations in the local community and beyond, in addition to their full-time jobs. This aligns with Restek’s True North philosophy that began with Restek’s founder and servant leader, Paul Silvis—to support the local communities in which we operate and make our community a better place to raise our children.

Restek is one of only six sponsors to receive the Gold Eagle Award—LCC’s highest level of recognition for sponsors

Restek has been a sponsor of LCC since the start. Our commitment to the program has been recognized by LCC with the Gold Eagle Award—their highest level of recognition for sponsors. Restek is one of only six sponsors to receive the award.

Mike Wittrig, R&D manager, will represent Restek for the 2018 LCC class.

For more information on LCC go to

Restek’s Commitment to the Community and the Environment Includes Orange Vests and Work Gloves

On an unseasonably chilly day in May, a team of eight Restek employees left together to do something the company has been doing twice a year since 2009—highway cleanup duty.

Restek employee-owners are all smiles after cleaning up our Adopt A Highway, two-mile section of Route 144.

For eight years, Restek employees have maintained a two-mile section of Route 144 running from Axemann to the Bellefonte Borough line as part of the PA Adopt A Highway program. Restek employees say it is a particularly important stretch of road because Logan Branch Creek—a Class A wild trout stream—runs along it.

“Keeping the area clean of litter helps keep the stream clean and productive, and we have a good time while we are doing it. Employees get to know each other a little better, and when we are done, we have lunch together,” Jim Richards, the cleanup crew organizer and calibration coordinator at Restek, said.

Seven employees joined Richards in the May cleanup, including his youngest son, Jacob Richards, who was recently hired at Restek. The other employee-volunteers were Mike Goss, Christina Hill, Jerry Johnston, Kendra Jozefick, Jessica Resides, and Brian Yangula.

As part of the program, employees can participate during the workday each spring and fall in support of the company’s environmental efforts and community service.

And it appears to be helping—Richards said the cleanup was lighter than most years.

“I would like to thank the many volunteers, past and present, that we have here at Restek that take the time to pick up someone else’s trash twice a year,” he said. “It doesn’t come with any kind of recognition or compensation, but it has its reward in serving the community.”

To learn more about Restek Corporation, visit

How to Avoid Common Problems with HILIC Methods

Author: Restek Corporation

Published By: Restek Corporation

Year of Publication: 2017



Whether you’ve run HILIC methods before or are attempting to for the first time, there are significant differences between reversed-phase and HILIC methods that must be considered. This article addresses common problems that analysts encounter when running HILIC methods and details how to avoid them and successfully use the technique.

Overcoming the Effects of Highly Organic Protein Precipitation Extracts on LC Peak Shape Using Direct Injection

Author: Sharon Lupo and Frances Carroll

Restek Corporation

Published By: Restek Corporation

Year of Publication: 2017



Protein precipitation is frequently used to minimize matrix impact when analyzing biological samples. However, the effects of highly organic protein precipitation sample extracts on LC peak shape can negatively impact accurate quantification. Dilution or further sample preparation steps are often used to minimize these effects; however, here we show that direct injection of sample extracts is a viable option that can be used to prevent peak distortion, while avoiding the time and variability associated with additional sample preparation.

Restek Employee-Owners Stay Active and Healthy with Corporate Olympics

And the global chromatography company officially opens a new fitness center and gym for its 350+ employees and their families

On June 1, Restek held the opening ceremonies for its corporate Olympics, a month-long competition that encourages employees to have fun, engage in friendly competition, and take ownership of their health—all in the spirit of the Wellness Team’s motto: “Live well. Work well.”

Beautiful sunny skies greeted three separate waves of runners at the 2017 Restek Plus1 5K.

The kickoff festivities were followed the next day by Restek’s 15th annual Plus1 5K. This year, the company even invited its health and wellness partners to join the race—almost 200 runners and walkers participated in total!—and after, employees visited with them to learn about available benefits and ask questions about services.

“The Restek Olympics are a great way for employees to take a break from their everyday jobs and have some fun with their coworkers. It is also a way to interact with coworkers you may not otherwise and have the opportunity to do so in a relaxed, ‘nonworking’ atmosphere,” Mike Perlozzo, the wellness coordinator at Restek, said.

A fun game of volleyball at Restek’s annual corporate Olympics (2015).

Throughout the month, teams of employees will compete in five events: volleyball, basketball, bean bag toss, pong, and a fitness challenge. Teams are each comprised of people from at least five different departments to allow stronger bonds to form throughout the organization.

But the Restek Olympics and Plus1 5K are just a few of many wellness events organized for employees and their families each year by the company, which is a leader in Centre County employee wellness programs. As a result, Restek and its employee-owners enjoy greater energy, productivity, healthy stress management, improved quality of life, and, over time, a reduction of more significant life-threatening diseases such as heart disease, diabetes, obesity, and cancer.

A variety of equipment options are available in Restek’s new fitness center.

Restek is also celebrating the official opening of its brand-new 17,000-square-foot gymnasium, called Founder’s Point. Last month, they installed the finishing touch—a custom-painted basketball floor.

“This space will allow employees and their families to enjoy a number of different activities such as basketball, soccer, and volleyball. There were a number of groups setting up times to use the space for soccer and basketball just minutes after we officially opened it. The response has been great, and the space will get a lot of use,” Perlozzo said.

The 17,000-square-foot-gymnasium offers indoor space for basketball, soccer, volleyball, and more.

Restek founder Paul Silvis started Restek Wellness in the late 1980s in an empty corner of their original building. They had only a universal weight machine and punching bag. By 2004, Restek hired its first full-time wellness coordinator, and in 2005 the official Restek Wellness Program was born. Silvis started Restek with a vision that happy, healthy employees will be more productive, be more successful, and help make the company more successful. Ever since, Restek has lived the success he envisioned, thanks in large part to the efforts of the Restek Wellness team.

To learn more about Restek Corporation, visit



High-Throughput Analysis of Immunosuppressive Drugs from Whole Blood by LC-MS/MS

Author: Shun-Hsin Liang and Frances Carroll

Restek Corporation

Published By: Restek Corporation

Year of Publication: 2017



The success of organ transplant therapy depends in large part on the accurate and timely analysis of immunosuppressive drugs. In this study, we developed a fast, accurate method for the analysis of cyclosporin A, tacrolimus, sirolimus, and everolimus in whole blood. The method pairs a single precipitation step with LC-MS/MS analysis using a Raptor Biphenyl column. A fast, 3-minute analysis time was obtained with no interference from matrix components. Excellent results were obtained for linearity, robustness, accuracy, and precision, demonstrating that the method is suitable for high-throughput therapeutic drug monitoring.

Direct Analysis of Morphine, M3G and M6G Metabolites, and Related Compounds in Urine by LC-MS/MS

Author: Justin Steimling and Frances Carroll

Restek Corporation

Published By: Restek Corporation

Year of Publication: 2017



The analysis of total morphine is typically conducted by first subjecting urine samples to acid or enzymatic hydrolysis in order to cleave the glucuronide conjugates from morphine’s primary metabolites [morphine-3β-D-glucuronide (M3G) and morphine-6β-D-glucuronide (M6G)] prior to analysis. With the glucuronide moiety removed, the resulting morphine molecule is much less polar and, therefore, more amenable to traditional reversed-phase chromatography. However, both hydrolysis procedures cost an analyst time and result in sample variability due to incomplete hydrolysis or analyte conversion. By utilizing the retention characteristics of the Restek Force Biphenyl column, hydrolysis was not required and direct analysis of morphine, its M3G and M6G metabolites, and several related compounds using a simple “dilute-and-shoot” sample preparation was performed.