Analysis of Amphetamines by LC-MS/MS for High-Throughput Urine Drug Testing Labs

Author: Ravali Alagandula and Frances Carroll

Published By: Restek Corporation

Year of Publication: 2019


Abstract: Accurate analysis of d- and l-amphetamine and methamphetamine enantiomers can be achieved using chiral columns, but the method presented here provides a simpler, more cost-effective approach for high-throughput labs. Excellent separation of all enantiomers in urine was obtained in a fast, 7-min LC-MS/MS quantitative analysis on a Raptor C18 column with no interference from matrix components.

Restek Named One of Region’s Healthiest Employers

Mike Perlozzo, Wellness Coordinator, accepts the Healthiest Employer Award

Restek was selected by the Healthiest Employers Awards as an employer committed to creating a healthy workplace.

The awards, administered by Springbuk, a healthcare data analytics software platform, honor organizations on a regional and national basis. Restek was selected as part of the Greater Philadelphia region, and Mike Perlozzo, Restek’s wellness coordinator, attended the ceremony on June 13 in Philadelphia. The company is also in the running to be selected as one of the 100 Healthiest Workplaces in America.

Restek employs three full-time wellness professionals who operate out of a recently built 17,000-square foot, top-of-the-line facility for employee-owners and their family members to enjoy fitness, basketball, volleyball, soccer, badminton, yoga, and many other activities.

“We are proud to be able to offer employees and their families opportunities to improve their health and wellness, and grateful to have the support of our leaders in doing so,” Perlozzo said.

In addition to fitness, the wellness team recently instituted a nutrition coaching program in 2016, and the number of employees meeting nutrition guidelines rose from 9.8% to 19.7% in two years.

Restek also pays the testing costs for biometric screening onsite for employees and their spouses. These tests include a standard lipid panel, glucose, A-1C (for diabetes type I and II), and a PSA prostate cancer screening for men 45 and older.

Restek founder Paul Silvis started the Restek wellness program in the late 1980s in an empty space in the original building with just a universal weight machine and punching bag. In 2004, Restek hired its first full-time wellness coordinator; in 2005 the official Restek Wellness Program was introduced; and in 2017, its designated wellness center, Founder’s Point, was built. Restek is currently the only company in Centre County with full-time health and fitness trainers.

For more information on the Healthiest Employers Awards, visit

Cannabidivarin (CBDV) Added to Restek’s Selection of Cannabis Reference Standards

Cannabis strains with high levels of cannabidiol (CBD) typically have higher levels of cannabidivarin (CBDV) as well, but accurate quantitation of both compounds for potency and strain ID is a must, especially as interest in using CBDV as an anticonvulsant and anti-nausea treatment continues to rise. Restek now offers a stand-alone CBDV reference standard—in addition to CBD and a host of other compounds—to help labs with the comprehensive analysis of cannabis products.

Restek’s CBDV standard is ideal for creating multipoint (5-point minimum suggested) calibration curves for LC-MS/MS. With verified composition and stability, this prepared stock product eliminates the need for in-house standards preparation for greater convenience and lower labor requirements. As a U.S. DEA-exempted formulation, no additional customer permits or licensing are required to purchase within the U.S., and because it’s manufactured and QC tested in Restek’s ISO-accredited labs, this new CBDV standard qualifies as a certified reference material (CRM) that satisfies your ISO requirements.

For CBDV, CBD, and other cannabis reference standards, along with the expertise and consumables your lab needs, turn to

Restek Celebrates Newest Home Dedication with Habitat for Humanity

“With the support of organizations like Restek our work is possible,” says Habitat executive director

When Habitat for Humanity of Greater Centre County (HFHGCC) dedicated its newest home on June 11, Restek Corporation received a special mention.

“With the support of organizations like Restek, our work is possible. Restek provided not only financial support, which is crucial to fund our safe and affordable housing, but also provided team members to our build site to give their time and talent. With the support of Restek, Habitat for Humanity of Greater Centre County is able to make another house into a home for a deserving family in Centre County,” said Stephanie Fost, HFHGCC Executive Director.

The home, located in Bellefonte, marked more than 60 families placed by Habitat in Centre County. Volunteers gave more than 3,000 hours, and the future homeowners put in over 450 hours of their own sweat equity into the home.

Restek encourages its employees to volunteer and permits exchanging four hours of work for volunteer work each month. One of those volunteers for the home build was Jason Martin, a Restek senior purchasing agent.

“I feel fortunate to work for a company that encourages their employees to help in their community. This was a perfect opportunity for me to contribute directly to the community that I lived in most of my life. Habitat for Humanity is a great organization with a great purpose, run by extraordinary people that truly care about the families they are providing these homes for. I’m sure I speak for the entire Restek team when I say it was a very rewarding experience,” he said.

Restek’s relationship with HFHGCC began many years ago and is currently championed by Mike Wittrig, Senior Column R&D Manager.

“Research shows that when families own a home, it makes all the difference in the world and stabilizes their life. It’s great that Restek helps support something like that, and I’m happy to play a small role,” Wittrig said.

For more information about Habitat for Humanity Great Centre County visit