Featured Pittcon Short Course: Practical Maintenance and Troubleshooting in Gas Chromatography

Jaap de Zeeuw

Jaap de Zeeuw

Date: Tuesday, March 03, 2020

Location: Pittcon 2020, Chicago

Course Description: 

In gas chromatography, 90% of the problems encountered can be traced back to issues in the injection system. In this half-day featured Pittcon short course, Restek’s Jaap de Zeeuw will discuss the purpose and impact of the critical parts used in split and splitless injection, as well as how they affect overall system maintenance schedules. Carrier gas choice and purity, tubing, connections, septa, ferrules, seals, liners, column coupling, column installation, and column maintenance all will be covered in detail. In addition, Jaap will discuss column operation/optimization concepts and explain how to extend GC column lifetime most effectively. At the end, we will explore a series of practical examples together through troubleshooting exercises.

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Webinar: How to Analyze Extended PFAS Lists Including Ultrashort-Chain (C2-C3) PFAS

Register today for this timely C&EN webinar about PFAS analysis and incorporating ultrashort-chain PFAS into an existing method for legacy and alternative compounds. Mike Chang, Global Environmental Market Business Development Manager at Restek, will discuss LC-MS/MS method development for C2 and C3 PFAS analysis and analytical methodologies for simultaneous chromatographic determination of ultrashort-chain, alternative, and legacy PFAS.

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New Resprep Polymeric SPE Offers an Alternative to Silica-Based Sorbents

Restek has expanded its line of Resprep solid phase extraction (SPE) products with the addition of polymeric sorbents, available in both cartridges for high loading capacity and 96-well plates for high throughput and automation.

Because Resprep polymeric SPE products feature a silica-free, bonded polymeric material, there are no unwanted secondary silica interactions, even with basic compounds. Their high surface area means a higher loading capacity compared to silica sorbents, and their stability over a wide pH range (0–14) means they won’t hydrolyze under extreme conditions. New Resprep polymeric SPE cartridges and 96-well plates are water-wettable, allowing for streamlined conditioning and equilibration steps to drastically reduce solvent usage and sample prep time. And they are not flow-rate dependent, so they maintain retention and capacity after conditioning, even if dried out from vacuum or positive pressure flows.

Resprep polymeric SPE products offer five distinct packings to choose from. Find them all, and the entire line of Resprep sample preparation products, at www.restek.com/resprep

Remove Both Phospholipids and Proteins in One Resprep PLR SPE Procedure

Simultaneously remove phospholipids and proteins in a single, simple procedure with new Resprep PLR (phospholipid removal) SPE products. Whole blood, serum, and plasma all contain proteins and phospholipids that can interfere with target analytes and hasten the need for instrument maintenance. It’s important to remove them from samples prior to analysis to avoid signal suppression, and Resprep PLR SPE cartridges or 96-well plates make this an easy task by combining protein precipitation and phospholipid removal in one sample preparation process. No analyte-specific method development is required because the same procedure can be used for samples containing acids, bases, or neutral compounds. In addition, effective removal of phospholipids and proteins from sample extracts reduces contamination, minimizing the frequency of instrument maintenance.


Phospholipid and Protein Removal in One Simple SPE Process Prevents Matrix-Based Signal Suppression

Published By: Restek Corporation

Year of Publication: 2020

Link: https://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/gen_GNAR3124-UNV

Abstract: Biological samples contain proteins and phospholipids that can interfere with LC-MS/MS analysis and increase the frequency of instrument maintenance. Here we used Resprep PLR SPE to simultaneously remove proteins and phospholipids from plasma in one high-efficiency sample preparation process. Concurrent phospholipid removal eliminated signal suppression and excellent recoveries were obtained for a wide range of chemical classes without the need for additional method development.