A Fast Dilute-And-Shoot Method for Simultaneous 5-Hydroxyindoleacetic Acid (5-HIAA), Vanillylmandelic Acid (VMA), and Homovanillic Acid (HVA) LC-MS/MS Analysis in Human Urine

Author(s): Shun-Hsin Liang and Sue Steinike
Restek Corporation

Published By: Restek Corporation

Year of Publication: 2016

Link: http://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/cft_CFAN2465-UNV

Abstract: In this partial validation study, we demonstrated the performance of a fast dilute-and-shoot LC-MS/MS method using a Raptor™ Biphenyl column for the simultaneous analysis of 5-hydroxyindoleacetic acid (5-HIAA), vanillylmandelic acid (VMA), and homovanillic acid (HVA) in human urine. Excellent results were obtained for linearity, accuracy, and precision. With this method, these serotonin and catecholamine metabolites can be measured in human urine with a 5-minute total analysis time. The analytical method is applicable to clinical labs that require a combination of fast analysis times and low-cost sample preparation procedures for 5-HIAA, VMA, and HVA using LC-MS/MS analysis.