Method Development and Column Selection: How the FluoroPhenyl Phase Provides the Power of HILIC and Reversed-Phase Modes in One Column

Author: Sharon Lupo, Frances Carroll

Restek Corporation

Published By: Restek Corporation

Year of Publication: 2017



Column selection is an important part of method development, as the quality of critical separations is determined in large part by the retention and selectivity of the analytical column. Although C18 columns are commonly employed, FluoroPhenyl columns provide an orthogonal alternative for method development because they incorporate multiple retention mechanisms and can be used in both reversed-phase (RP) and hydrophilic interaction liquid chromatography (HILIC) modes. This paper explores how FluoroPhenyl columns can be used to improve separations, especially for compounds that are difficult to analyze on a C18, such as basic analytes or small polar compounds.