Trace-Level Analysis of Metanephrines in Plasma by HILIC LC-MS/MS

Author: Shun-Hsin Liang and Frances Carroll

Published By: Restek Corporation

Year of Publication: 2017



Highly sensitive analysis of metanephrines and in plasma is critical in the diagnosis and treatment of pheochromocytoma and paraganglioma. Here, a HILIC LC-MS/MS method was developed using a Raptor HILIC-Si column because this approach provides retention of all target compounds in highly organic mobile phases, thus providing the increased sensitivity needed to reach low detection limits. Data from linearity, accuracy, and precision testing demonstrate that accurate results are consistently obtained—even at trace levels—for these important clinical biomarkers.