Simplified Methylmalonic Acid Analysis without Derivatization in Human Plasma by LC-MS/MS

Published By: Restek Corporation

Year of Publication: 2018



Methylmalonic acid analysis is an important clinical test for diagnosing vitamin B12 deficiency as well as methylmalonic acidemia. Methods typically require extensive sample preparation, which involves significant time and expense. The LC-MS/MS method developed here offers a much simpler alternative, providing complete chromatographic resolution of isobaric analytes from the direct injection of a protein crash sample supernatant onto a Force C18 column.