Leveraging Selectivity and Efficiency to Take the Strain Out of LC–UV Method Development for Cannabinoid Profiling

Author(s): Justin Steimling
Restek Corporation

Published By: Cannabis Science and Technology

Issue: Volume 1, Issue 4

Year of Publication: 2018

Link: http://www.cannabissciencetech.com/article/leveraging-selectivity-and-efficiency-take-strain-out-lc%E2%80%93uv-method-development-cannabinoid

Abstract: More than 100 cannabinoids have been isolated from cannabis in addition to the five most commonly tested: ∆9-tetrahydrocannabinol (∆9-THC), ∆9-tetrahydrocannabinolic acid (THCA), cannabidiol (CBD), cannabidiolic acid (CBDA), and cannabinol (CBN). Although many methods have been published that show the separation of these major cannabinoids, most do not take into account the possibility of interference from minor cannabinoids. This interference is most problematic in concentrates where minor cannabinoids can be enriched to detectable levels. Additionally, some terpenes absorb ultraviolet (UV) light at the same wavelength as cannabinoids, which can result in an additional source of interference. In this study, the liquid chromatography (LC)–UV separation of 16 cannabinoids of interest was performed while the potential impact from minor cannabinoids and terpenes on reported potency values was monitored. The method was applied to commercially available CBD oils that have recently become suspect because of inaccurate label claims.