Evaluation of a Simple Method for Methylmalonic Acid Analysis in Human Plasma by LC-MS/MS

Author: Justin Steimling and Frances Carroll

Published By: Restek Corporation

Year of Publication: 2019

Link: https://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/cft_CFAN3010-UNV


A new LC-MS/MS method for methylmalonic acid (MMA) analysis was developed that provides complete chromatographic resolution from isobaric succinic acid in plasma samples. Excellent chromatographic results were obtained from the direct injection of a protein crash sample supernatant onto a Force C18 column, providing a much simpler sample preparation compared to typical methods.