Phospholipid and Protein Removal in One Simple SPE Process Prevents Matrix-Based Signal Suppression

Published By: Restek Corporation

Year of Publication: 2020

Link: https://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/gen_GNAR3124-UNV

Abstract: Biological samples contain proteins and phospholipids that can interfere with LC-MS/MS analysis and increase the frequency of instrument maintenance. Here we used Resprep PLR SPE to simultaneously remove proteins and phospholipids from plasma in one high-efficiency sample preparation process. Concurrent phospholipid removal eliminated signal suppression and excellent recoveries were obtained for a wide range of chemical classes without the need for additional method development.