Simultaneous Analysis of Catecholamines and Metanephrines in Urine by LC-MS/MS

Author: Shun-Hsin Liang and Frances Carroll

Published By: Restek Corporation

Year of Publication: 2017

Link: http://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/cft_CFAN2800-UNV

Abstract: 

Clinical diagnosis of pheochromocytoma and paraganglioma is often based on the analysis of catecholamines (epinephrine, norepinephrine, dopamine) and metanephrines (metanephrine, normetanephrine, 3-methoxytyramine) in urine. Analysis of these polar compounds using reversed-phase LC can be difficult due to limited chromatographic retention, which results in poor separation of the analytes from closely eluting matrix interferences. This method overcomes these problems by combining a simple solid phase extraction procedure with the consistent and accurate chromatographic performance of a Raptor Biphenyl column.

Analysis of Fentanyl and Its Analogues in Human Urine by LC-MS/MS

Author: Shun-Hsin Liang and Frances Carroll

Published By: Restek Corporation

Year of Publication: 2017

Link: http://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/cft_CFAN2820-UNV

Abstract: 

Synthetic opioid drugs, such as fentanyl and sufentanil, have very high analgesic potency. Abuse of these prescription painkillers—along with a rapidly growing list of illicit analogues—is a significant public health problem. In this study, we developed a simple dilute-and-shoot method that provides a fast 3.5 minute analysis of fentanyl and related compounds (norfentanyl, acetyl fentanyl, alfentanil, butyryl fentanyl, carfentanil, remifentanil, and sufentanil) in human urine by LC-MS/MS using a Raptor Biphenyl column.

Forensic Characterization of Drug Exposure from Skeletal Remains (Featuring Raptor Biphenyl LC columns)

Author: James Watterson

Published By: SelectScience

Year of Publication: 2016

Link: http://www.selectscience.net/editorial-articles/forensic-characterization-of-drug-exposure-from-skeletal-remains/?artID=42183#.WAeNLLeGnOE.twitter

Abstract:

Dr. James Watterson, Associate Professor, Department of Forensic Science at Laurentian University in Ontario, told SelectScience about his forensic toxicology research and the technologies he utilizes in his work. Dr. Watterson’s primary interest is the characterization of drug and metabolite disposition in skeletal remains, and he has employed a variety of chromatography and mass spectrometry-based analyses. The team is now investigating the probative power of quantitative drug-metabolite relationships in bone using LC-MS techniques, which offer greater sensitivity and selectivity for analyses of a number of different metabolites. As Dr. Watterson explains, “Now that we have switched to UPLC-qTOF-MS as our primary analytical approach, the advantages are absolute: substantially reduced sample preparation requirements, vastly improved sensitivity and selectivity, and a much greater of analytes that may be assayed.” The choice of LC column for such analyses is also of critical importance, the correct column chemistry is essential for efficient separation. For example, the team used Raptor Biphenyl columns, with long column geometry and small particle/fused-core stationary phase, to maximize resolution of polar metabolites of phenothiazine drugs, using UPLC-PDA. This approach was critical in the characterization of these metabolites and of the phenothiazine oxidation products, which are produced during sample preparation. Dr. Watterson comments that, “the biphenyl column has been very helpful in resolving those compounds too, as we applied this method to UPLC-qTOF-MS.” Dr. Watterson advises choosing reagents and columns carefully, as background impurities are much more visible using current technologies.

Our MSACL U.S. 2015 Presentations Are Now Online

During MSACL U.S. 2015, we featured Restek’s Raptor™ Biphenyl column in our booth, corporate workshop, and posters. With ten years of unrivaled performance, Restek’s time-tested Biphenyl has long been the ideal column for clinical diagnostic, pain, and pharma labs alike. Pairing it with rugged Raptor™ particles makes it your best choice for fast, reliable results.

For those who weren’t able to catch all of our poster presentations, we have uploaded them to our MSACL page. Review them at your leisure, and feel free to get in touch with the authors directly to discuss them further.

www.restek.com/msacl-us

Restek at MSACL US

A Rapid and Sensitive LC-MS/MS Method for the Analysis of Three Forms of Thyroid Hormones Using Raptor™ Biphenyl LC Columns

Author(s): Shun-Hsin Liang, Paul Connolly, and Ty Kahler
Restek Corporation

Published By: Restek Corporation

Year of Publication: 2014

Link: http://www.restek.com/Technical-Resources/Technical-Library/Bioanalytical/bio_BAAN2112-UNV

Abstract: The Raptor™ Biphenyl column is excellent for rapid and sensitive analysis of thyroid hormones. With the method described here, concentrations of thyroid hormones as low as 2 pg/mL (T3) or 5 pg/mL (T4 and rT3) can be determined with less than 3.5 minutes of total analysis time. The analytical method is thus applicable to the clinical analysis of free thyroid hormone at low pg/mL levels.

Experience Selectivity Accelerated With New Raptor™ SPP LC Columns and Guards

Restek is excited to announce the evolution of superficially porous particles with the introduction of Raptor™ LC columns.

Raptor™ LC ColumnsSuperficially porous particles (commonly referred to as SPP or “core-shell” particles) changed the world of LC by dramatically boosting column efficiency and reducing analysis times, but they were only the beginning. With Raptor™ LC columns, Restek chemists have combined the speed of SPP with the resolution of highly selective USLC® technology. This new species of chromatographic column allows you to more easily achieve peak separation and faster analysis times without expensive UHPLC instrumentation. Raptor™ LC columns provide the practicing analyst with the most powerful tools available for fast, efficient method development and increased sample throughput. And because they are from Restek, Raptor™ LC columns are backed by manufacturing and quality systems you trust along with internationally renowned Plus 1 service.

Restek BiphenylThe first phase to be rolled out is the innovative Biphenyl, which will be followed by a full suite of selective USLC® phases. The industry-leading Biphenyl is Restek’s most popular LC stationary phase because it is particularly adept at separating compounds that are hard to resolve or that elute early on C18 and other phenyl chemistries. As a result, the rugged Raptor™ Biphenyl column is extremely useful for fast separations in bioanalytical testing applications like drug and metabolite analyses, especially those that require a mass spectrometer (MS). Increasing retention of early-eluting compounds can limit ionization suppression, and the heightened selectivity also helps eliminate the need for complex mobile phases that are not well suited for MS detection. Restek was the first to bring chemists the benefits of the Biphenyl ligand and has the knowledge to maximize SPP performance of this premier phenyl chemistry.

To help further extend the life of already-rugged Raptor™ LC columns, Restek has also mated these new superficially porous particles with patent-pending guard column hardware developed by Optimize TechnoloRaptor™ EXP® Guard Columnsgies. Sold separately and available in three different IDs, the Raptor™ LC guard column cartridge in an EXP® direct connect holder is the ultimate in column protection. Change cartridges without breaking inlet/outlet fluid connections—and without tools.

Experience Selectivity Accelerated. Put Raptor™ LC columns and guards to the test today on your most challenging workflows!

www.restek.com/raptor

Restek QuEChERS SPE Tubes and Biphenyl LC Columns Honored by Laboratory Equipment Magazine

2009 Laboratory Equipment WinnerTwo Restek product lines were recently honored by Laboratory Equipment magazine. QuEChERS SPE tubes were selected as a winner and Biphenyl LC columns were named as a finalist in the magazine’s 2009 Reader’s Choice Awards competition. Winners were selected by practicing scientists and engineers based on the value and performance of the products in research labs. For more information on the advantages of QuEChERS SPE tubes visit www.restek.com/quechers and to learn how Biphenyl columns can improve your separations visit www.restek.com/biphenyl