Fast, Accurate LC-MS/MS Method for Folate Deficiency Biomarkers in Plasma

Published By: Restek Corporation

Year of Publication: 2017



LC-MS/MS methods for folate deficiency biomarkers in plasma are complicated by the presence of matrix phospholipids and lysophospholipids. These interferences are problematic with reversed-phase LC because they can suppress response, reduce accuracy, and contaminate the MS source. Using a Raptor HILIC-Si column and the HILIC method shown here, folate deficiency biomarkers are completely separated from matrix interferences, allowing accurate determinations to be made in a fast, 5-minute analysis.