When Do We Need Sub-2-µm Superficially Porous Particles for Liquid Chromatography Separations?

Authors: David S. Bell, Landon Wiest, Shun-Hsin Liang, Dan Li

Restek Corporation

Published By: LCGC North America

Year of Publication: July 2018

Volume, Issue: Volume 36, Issue 7

Link: www.chromatographyonline.com/view/when-do-we-need-sub-2-m-superficially-porous-particles-liquid-chromatography-separations

Abstract: The use of superficially porous particles (SPPs) for modern high performance liquid chromatography (HPLC) is now very common. Initially, SPPs rose as an alternative to sub-2-µm fully porous particles (FPPs). In recent years, many column manufacturers have developed 2-µm and smaller SPP-based products. This article investigates the practical utility of these smaller SPP designs.

Instrumentation Used for Terpene Analysis

Author: Tim Herring

Restek Corporation

Published By: Cannabis Industry Journal

Year of Publication: 2017

Link: https://www.cannabisindustryjournal.com/column/instrumentation-used-for-terpene-analysis/


Because terpenes are somewhat volatile, the Restek team recommends using gas chromatography and advises against using HPLC for terpene analysis.

Many customers ask technical service which instrumentation is best, GC or HPLC, for analysis of terpenes. Terpenes are most amenable to GC, due to their inherent volatility. HPLC is generally not recommended; since terpenes have very low UV or MS sensitivity; the cannabinoids (which are present in percent levels) will often interfere or coelute with many of the terpenes.

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Effects of Column Inner Diameter and Packed Bed Heterogeneities on Chromatographic Performance

Authors: Edward G. Franklin and Ty W. Kahler
Restek Corporation

Published By: LCGC North America

Year of Publication: 2016

Link: http://www.chromatographyonline.com/effects-column-inner-diameter-and-packed-bed-heterogeneities-chromatographic-performance

For the full issue, visit: http://images2.advanstar.com/PixelMags/lcgc-na/digitaledition/06-2016.html#18


In recent years, industry has been moving to columns with smaller and smaller inner diameters—moving from 4.6 and 3.0 mm i.d. columns to 2.1 mm, 1.0 mm, and even smaller. While small inner diameter columns have some clear advantages, they also bring challenges.  Reduction of extracolumn volumes must be given greater consideration by both customers and manufacturers. This installment of “Column Watch” focuses on the sources of band broadening within high performance liquid chromatography (HPLC) columns with an emphasis on eddy dispersion. The physical mechanisms of dispersion are discussed and a review of the current literature as it pertains to small inner diameter columns is presented.