“The Big Pain”: Development of Pain-Free Methods for Analyzing 231 Multiclass Drugs and Metabolites by LC-MS/MS

Author(s): Sharon Lupo
Restek Corporation

Published By: Restek Corporation

Year of Publication: 2015

Link: http://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/cft_CFAR2309-UNV

Abstract: As the demand for testing of pain management drugs increases, many laboratories are turning to LC-MS/MS for its increased speed, sensitivity, and specificity. The methods shown here use a Raptor™ Biphenyl column because it provides fast, accurate analysis of 231 drugs and drug metabolites.

New Raptor™ ARC-18 Columns Are Born for LC-MS/MS

The birth of Restek’s new Raptor™ SPP LC column line began with the innovative Biphenyl phase, and it has now grown to include a new Restek® phase: the ARC-18.

Raptor™ LC ColumnsRaptor™ ARC-18 ColumnDesigned and intended specifically for use on LC-MS/MS systems, the Raptor™ ARC-18 column offers a well-balanced retention profile without the drawbacks of using an ordinary C18 in the harsh, acidic mobile phases needed for mass spectrometry (MS). Even after extended use in these low-pH (≤ 2.0) conditions, the sterically protected ARC-18 offers consistent retention, peak shape, and response for charged bases, neutral acids, small polar compounds, and more. For the rapid analysis of large, multiclass assays by LC-MS/MS, the Raptor™ ARC-18 truly is ahead of the curve.



About Raptor™ SPP LC Columns: Superficially porous particles (commonly referred to as SPP or “core-shell” particles) changed the world of LC by dramatically boosting column efficiency and reducing analysis times, but they were only the beginning. With Raptor™ LC columns, Restek chemists have combined the speed of SPP with the resolution of highly selective USLC® technology. This new species of chromatographic column allows you to more easily achieve peak separation and faster analysis times without expensive UHPLC instrumentation. Raptor™ LC columns provide the practicing analyst with the most powerful tools available for fast, efficient method development and increased sample throughput. And because they are from Restek, Raptor™ LC columns are backed by manufacturing and quality systems you trust along with internationally renowned Plus 1 service.

Accurately Detect and Quantify 204 Pesticides of Global Food Safety Concern by LC-MS/MS

Restek LC-MS/MS Multi-Residue Pesticide KitStop spending your time and money developing LC-MS/MS multi-residue pesticide methods and mixing reference standards when you could be running samples! Restek’s trusted food safety experts have already done the work for you—with guaranteed accuracy and at a lower cost.

Restek’s new certified reference materials (CRMs) for LC-MS/MS multi-residue pesticide analysis eliminate the need to source, purchase, and store individual materials along with the need to prepare your own mixes. Choose separate ampuls for mix-and-match customization or a complete kit that contains 204 compounds of interest, covering many LC-determined pesticides listed by government agencies. Designed for maximum shelf life, stability, and chromatographic performance, each standard ships with support documentation detailing Restek’s rigorous in-house quantitative testing. They even offer an optimized multi-residue pesticide method—including conditions and downloadable transition tables—free of charge.

Set up, verify, and validate your pesticide-screening methods in less time, with proven confidence, and at a significantly reduced cost by turning to Restek’s ISO-accredited labs for your LC-MS/MS multi-residue pesticide certified reference materials. For added convenience and unbeatable results, also consider their internal standards, Ultra and Pinnacle® DB LC columns, and Q-sep™ QuEChERS sample prep products.

Learn more at www.restek.com/LC-multiresidue