Analysis of Pesticides and Mycotoxins in Cannabis Brownies

Author: Nathaly Reyes and Colton Myers

Published By: Restek Corporation

Year of Publication: 2020

Link: https://www.restek.com/Technical-Resources/Technical-Library/Foods-Flavors-Fragrances/fff_FFAN3149-UNV

Abstract: Government regulations in California require the cannabis industry to test edible products for an extensive list of pesticides and mycotoxins. Here, an effective workflow for this complex analysis was developed in brownies and optimization strategies are detailed in order to provide a starting point for similar matrices. Both LC-MS/MS and GC-MS/MS were employed and excellent results for LOQ, linearity, accuracy, and precision were attained for all the target compounds.

Can Nonmatching Mycotoxin SIDA Internal Standards Be Used for Stable Isotope Dilution Assay Calibration?

Published By: Restek Corporation

Year of Publication: 2019

Link: https://www.restek.com/Technical-Resources/Technical-Library/Foods-Flavors-Fragrances/fff_FFAR3036-UNV

Abstract:

Stable isotope dilution assay (SIDA) is a calibration technique that can be used to offset matrix effects. However, care must be given to choosing appropriate internal standards. This article demonstrates the importance of using a labeled internal standard that matches the target analyte for mycotoxins analysis.

Quantitation of Mycotoxins in Four Food Matrices Comparing Stable Isotope Dilution Assay (SIDA) with Matrix-Matched Calibration Methods by LC–MS/MS

Author(s): Dan Li; Justin A. Steimling; Joseph D. Konschnik, Scott L. Grossman; Ty W. Kahler
Restek Corporation

Published By: Journal of AOAC International

Year of Publication: 2019

Link: https://www.ingentaconnect.com/content/aoac/jaoac/pre-prints/content-jaoac_190028

Abstract: Background: Mycotoxins are big concerns in food safety. Analytical methods are important for the evaluation of mycotoxins in different food commodities. Objective: In this study, stable isotope dilution assay (SIDA) was compared with a matrix-matched calibration method for the quantification of mycotoxins in four different commercially available commodities and two reference materials. Methods: All samples were extracted with water–acetonitrile (50+50, v/v), followed by filtration and LC–tandem MS analysis. Results: SIDA calibration accuracies ranged from 78.6 to 112% with relative SDs (RSDs) ≤16% across all four matrices. The majority of the recoveries across all matrices ranged from 70 to 120% with RSDs <11%. Of the four mycotoxins in the reference materials analyzed, only three had 13C-internal standard (IS), whereas the fourth was quantified using a closely eluting 13C-IS for a different mycotoxin. Mycotoxins paired with their corresponding 13C-IS had accuracies >90%, whereas accuracies for the mismatched mycotoxin/13C-IS were <14%. Conclusions: When 13C-IS are not available, matrix-matched calibration was also evaluated as an alternative to quantitating target mycotoxins. The use of 13C-IS is the best way to dynamically account for prevalent matrix effects, but matrix matching provides a viable alternative. Highlights: The study compared SIDA and matrix-matched calibration methods in terms of recovery, efficiency, advantages, and limitations for LC-MS based mycotoxin analysis

5.5 Minute LC-MS/MS Analysis of Mycotoxins in Peanut Powder

Published By: Restek Corporation

Year of Publication: 2018

Link: https://www.restek.com/Technical-Resources/Technical-Library/Foods-Flavors-Fragrances/fff_FFSS2971-UNV

Abstract:The method developed here provides a fast analysis of mycotoxins in peanut powder. Baseline separation of 12 FDA and EU regulated mycotoxins was achieved in a 5.5 minute analysis (7 minute total cycle time). This method can be applied to different matrices using either matrix-matched calibration or a stable isotope dilution assay. Sample throughput can be greatly increased by the short cycle time for each run.

High-Throughput Analysis of Mycotoxins in Cannabis CBD Oil Pairs Simplified Cleanup with LC-MS/MS Sensitivity

Published By: Restek Corporation

Year of Publication: 2018

Link: http://www.restek.com/Technical-Resources/Technical-Library/Foods-Flavors-Fragrances/fff_FFSS2946-UNV

Abstract: Complex, time-consuming immunoaffinity column sample prep protocols for mycotoxins can be replaced by this simple pass-through SPE sample cleanup. Paired with LC-MS/MS analysis using a Raptor Biphenyl column, excellent chromatographic results were obtained for aflatoxins and ochratoxins at 2 ng/g, providing a high-throughput solution for analyzing mycotoxins in cannabis.

Highly Selective LC-MS/MS Analysis of Mycotoxins from Multiple Classes

Published By: Restek Corporation

Year of Publication: 2017

Link: http://www.restek.com/Technical-Resources/Technical-Library/Foods-Flavors-Fragrances/fff_FFSS2790-UNV

Abstract: Mycotoxin contamination of crops is not only a public health concern, but it also has a huge impact on the global economy. This highly selective LC-MS/MS analysis of 20 mycotoxins in just 9 minutes allows scientists to accurately identify toxins, to monitor food products for contamination, and to support control strategies that prevent economic loss.