Influencing Selectivity in LC-MS Peptides Analysis on the Raptor ARC-18 Column

Author: Sharon Lupo, Frances Carroll

Restek Corporation

Published By: Restek Corporation

Year of Publication: 2017

Link: http://www.restek.com/Technical-Resources/Technical-Library/Pharmaceutical/pharma_PHAN2615-UNV

Abstract:

Methods for LC-MS peptide analysis often use acidified mobile phases, which can alter retention and selectivity. Here, we demonstrate the effects of modifying acid type, acid concentration, temperature, and gradient slope using several test probes. A Raptor ARC-18 column was employed for this work because the stationary phase ligand is sterically protected, making it extremely stable and resistant to acid damage at low pHs, which is an important characteristic when developing LC-MS methods for peptide analysis.