Novel Stationary Phase for Comprehensive PFAS Analysis: Ultrashort-Chain (C2, C3), Alternative, and Legacy Compounds

Published By: Restek Corporation

Year of Publication: 2020

Link: https://www.restek.com/Technical-Resources/Technical-Library/Environmental/env_EVFA3197-UNV

Abstract: In this fast, isocratic LC-MS/MS analysis, Raptor Polar X columns provide proper chromatographic retention of TFA and other small, polar ultrashort-chain PFAS as well as short- and long-chain PFAS.

Simple Large Volume Injection Method for Trace-Level Glyphosate in Bottled Drinking Water

Published By: Restek Corporation

Year of Publication: 2020

Link: https://www.restek.com/Technical-Resources/Technical-Library/Environmental/env_EVFA3196-UNV

Abstract: Trace-level detection of glyphosate can be achieved without complex derivatization or ion paining by using direct large volume injection of water on a Raptor Polar X column.

Raptor Polar X: Separate a Wide Variety of Polar Analytes with a Novel Hybrid Stationary Phase

Published By: Restek Corporation

Year of Publication: 2020

Link: https://www.restek.com/Technical-Resources/Technical-Library/General-Interest/gen_GNSS3195-UNV

Abstract: The unique hybrid phase in Raptor Polar X columns balances HILIC and ion exchange retention modes, making it ideal for analyzing a wide range of polar compounds, especially when coupled with mass spectrometry.

Separate a Wide Variety of Polar Analytes with New Raptor Polar X Columns

Analyzing polar compounds using liquid chromatography has historically been a challenge. Poor retention and peak shape, complex mobile phases that may not be MS-friendly, long equilibration times, low sensitivity, and sample derivatization are all complications that reduce lab efficiency and productivity. However, new Raptor Polar X columns reliably retain and efficiently separate a broad range of polar analytes using a unique stationary phase that balances two retention mechanisms: HILIC and ion exchange. This hybrid phase is ideal for analyzing acidic, basic, and neutral polar compounds without time-consuming derivatization or complex ion pairing, and it is especially compatible with mass spectrometry. Easy changes to mobile phase conditions allow analysts to switch between modes and selectively tune retention for the compounds of interest without needing long equilibration times. Simplify the analysis of polar compounds with the resolving power of Raptor Polar X columns from Restek.

Learn more about Raptor Polar X columns at www.restek.com/PolarX

About Raptor SPP LC Columns:
Restek chemists became the first to combine the speed of superficially porous particles (also known as SPP or “core-shell” particles) with the resolution of highly selective USLC technology. This new breed of chromatographic column allows you to more easily achieve peak separation and faster analysis times without expensive UHPLC instrumentation.

Learn more about Raptor LC columns at www.restek.com/raptor

Attain a More-Comprehensive Characterization of Cannabis Samples with Restek’s New Cannabicyclol (CBL) Reference Standard

Cannabicyclol (CBL) is formed through the photodegradation of cannabichromene (CBC). Levels are typically quite low, and the medicinal benefits of CBL are still being researched, but quantifying this compound in cannabis samples is crucial for obtaining a more-complete cannabinoids profile. Restek has just released a new CBL certified reference material (CRM) to further aid labs in the comprehensive characterization of cannabis: www.restek.com/catalog/view/54615

Restek’s new CBL standard is ideal for creating multipoint (5-point minimum suggested) calibration curves for LC analysis. With verified composition and stability, this prepared stock product eliminates the need for in-house standards preparation for greater convenience and lower labor requirements. As a U.S. DEA-exempted formulation, no additional customer permits or licensing are required to purchase within the U.S., and because it’s manufactured and QC tested in Restek’s ISO-accredited labs, this new CBL standard qualifies as a certified reference material (CRM) that satisfies your ISO requirements.

For CBL and other reference standards, Raptor LC columns, sample preparation products, and the expert consultation your cannabis lab needs, turn to www.restek.com/cannabis

PFAS LC Column Anatomy: Which Phase, Dimensions, and Particle Type Are Best?

Published By: Restek Corporation

Year of Publication: 2019

Link: https://www.restek.com/Technical-Resources/Technical-Library/Environmental/env_EVAR3069-UNV

Abstract: With the expansion of PFAS target analyte lists into shorter chain compounds, the need for better methods is growing. But, when developing new analytical procedures, which PFAS LC column is best? This article explores the impact of phase, dimensions, and particle types and provides options that ensure fast, accurate results.

Successful Strategies for the Analysis of EtG and EtS in Urine: Rugged Sample Preparation and Analysis Conditions for High-Throughput Labs

Author: Justin Steimling, Ravali Alagandula, and Frances Carroll

Published By: Restek Corporation

Year of Publication: 2019

Link: https://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/cft_CFAR3061-UNV

Abstract: LC-MS/MS analysis of EtG and EtS is challenging due primarily to poor retention and resolution of EtG and EtS from matrix components. This article presents a foundation method that is useful for many samples and also demonstrates effective strategies for improving sensitivity and mitigating matrix effects.

Rapid, Simple 4.5-Minute Pyrrolizidine Alkaloids Analysis by LC-MS/MS

Published By: Restek Corporation

Year of Publication: 2019

Link: https://www.restek.com/Technical-Resources/Technical-Library/Foods-Flavors-Fragrances/fff_FFSS3064-UNV

Abstract: Pyrrolizidine alkaloid analysis is time consuming, with a typical run time of 15 minutes, and the acid digestion step can shorten analytical column lifetime. In contrast, this analysis using an acid-resistant Raptor ARC-18 column eluted the final compound in just 3.2 minutes with good peak shape and separation.

Analysis of Amphetamines by LC-MS/MS for High-Throughput Urine Drug Testing Labs

Author: Ravali Alagandula and Frances Carroll

Published By: Restek Corporation

Year of Publication: 2019

Link: https://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/cft_CFAN3052-UNV

Abstract: Accurate analysis of d- and l-amphetamine and methamphetamine enantiomers can be achieved using chiral columns, but the method presented here provides a simpler, more cost-effective approach for high-throughput labs. Excellent separation of all enantiomers in urine was obtained in a fast, 7-min LC-MS/MS quantitative analysis on a Raptor C18 column with no interference from matrix components.

Method Evaluation: Analysis of Phosphatidylethanol (PEth) in Human Whole Blood by LC-MS/MS

Author: Shun-Hsin Liang and Frances Carroll

Published By: Restek Corporation

Year of Publication: 2019

Link: https://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/cft_CFAN3009-UNV

Abstract:

The method established in this study provides accurate analysis of phosphatidylethanol (PEth) using a simple protein precipitation procedure and a fast 3.5 minute LC-MS/MS gradient run. Specific, sensitive analysis of PEth 16:0/18:1 in whole blood was obtained due to the unique selectivity and retention of the Raptor FluoroPhenyl column.