Attain a More-Comprehensive Characterization of Cannabis Samples with Restek’s New Cannabicyclol (CBL) Reference Standard

Cannabicyclol (CBL) is formed through the photodegradation of cannabichromene (CBC). Levels are typically quite low, and the medicinal benefits of CBL are still being researched, but quantifying this compound in cannabis samples is crucial for obtaining a more-complete cannabinoids profile. Restek has just released a new CBL certified reference material (CRM) to further aid labs in the comprehensive characterization of cannabis: www.restek.com/catalog/view/54615

Restek’s new CBL standard is ideal for creating multipoint (5-point minimum suggested) calibration curves for LC analysis. With verified composition and stability, this prepared stock product eliminates the need for in-house standards preparation for greater convenience and lower labor requirements. As a U.S. DEA-exempted formulation, no additional customer permits or licensing are required to purchase within the U.S., and because it’s manufactured and QC tested in Restek’s ISO-accredited labs, this new CBL standard qualifies as a certified reference material (CRM) that satisfies your ISO requirements.

For CBL and other reference standards, Raptor LC columns, sample preparation products, and the expert consultation your cannabis lab needs, turn to www.restek.com/cannabis

PFAS LC Column Anatomy: Which Phase, Dimensions, and Particle Type Are Best?

Published By: Restek Corporation

Year of Publication: 2019

Link: https://www.restek.com/Technical-Resources/Technical-Library/Environmental/env_EVAR3069-UNV

Abstract: With the expansion of PFAS target analyte lists into shorter chain compounds, the need for better methods is growing. But, when developing new analytical procedures, which PFAS LC column is best? This article explores the impact of phase, dimensions, and particle types and provides options that ensure fast, accurate results.

Successful Strategies for the Analysis of EtG and EtS in Urine: Rugged Sample Preparation and Analysis Conditions for High-Throughput Labs

Author: Justin Steimling, Ravali Alagandula, and Frances Carroll

Published By: Restek Corporation

Year of Publication: 2019

Link: https://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/cft_CFAR3061-UNV

Abstract: LC-MS/MS analysis of EtG and EtS is challenging due primarily to poor retention and resolution of EtG and EtS from matrix components. This article presents a foundation method that is useful for many samples and also demonstrates effective strategies for improving sensitivity and mitigating matrix effects.

Rapid, Simple 4.5-Minute Pyrrolizidine Alkaloids Analysis by LC-MS/MS

Published By: Restek Corporation

Year of Publication: 2019

Link: https://www.restek.com/Technical-Resources/Technical-Library/Foods-Flavors-Fragrances/fff_FFSS3064-UNV

Abstract: Pyrrolizidine alkaloid analysis is time consuming, with a typical run time of 15 minutes, and the acid digestion step can shorten analytical column lifetime. In contrast, this analysis using an acid-resistant Raptor ARC-18 column eluted the final compound in just 3.2 minutes with good peak shape and separation.

Analysis of Amphetamines by LC-MS/MS for High-Throughput Urine Drug Testing Labs

Author: Ravali Alagandula and Frances Carroll

Published By: Restek Corporation

Year of Publication: 2019

Link: https://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/cft_CFAN3052-UNV

Abstract: Accurate analysis of d- and l-amphetamine and methamphetamine enantiomers can be achieved using chiral columns, but the method presented here provides a simpler, more cost-effective approach for high-throughput labs. Excellent separation of all enantiomers in urine was obtained in a fast, 7-min LC-MS/MS quantitative analysis on a Raptor C18 column with no interference from matrix components.

Method Evaluation: Analysis of Phosphatidylethanol (PEth) in Human Whole Blood by LC-MS/MS

Author: Shun-Hsin Liang and Frances Carroll

Published By: Restek Corporation

Year of Publication: 2019

Link: https://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/cft_CFAN3009-UNV

Abstract:

The method established in this study provides accurate analysis of phosphatidylethanol (PEth) using a simple protein precipitation procedure and a fast 3.5 minute LC-MS/MS gradient run. Specific, sensitive analysis of PEth 16:0/18:1 in whole blood was obtained due to the unique selectivity and retention of the Raptor FluoroPhenyl column.

Comprehensive LC-MS/MS Analysis of 15 Bisphenols in 8 Minutes

Published By: Restek Corporation

Year of Publication: 2018

Link: http://www.restek.com/Technical-Resources/Technical-Library/Foods-Flavors-Fragrances/fff_FFSS2935-UNV

Abstract: The analysis of bisphenolic compounds that are used as alternatives to bisphenol A (BPA) is essential for monitoring exposure to and evaluating the safety of these emerging industrial chemicals. The LC-MS/MS method presented here provides excellent chromatographic peak shape and separation for an extensive list of BPA analogues in a fast, 8-minute analysis.

Fast, Simple LC-MS/MS Analysis of Creatine and Creatinine

Published By: Restek Corporation

Year of Publication: 2018

Link: http://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/cft_CFSS2937-UNV

Abstract: A rapid, robust method was established for the analysis of creatine and creatinine in human plasma and urine. An efficient separation was achieved in less than 1.5 min using a Raptor HILIC-Si column and a simple isocratic mobile phase.

A Novel Solution for Vitamin K1 and K2 Analysis in Human Plasma by LC-MS/MS

Author: Shun-Hsin Liang and Frances Carroll

Published By: Restek Corporation

Year of Publication: 2018

Link: http://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/cft_CFAN2934-UNV

Abstract: Vitamin K1 and K2 analysis is typically complex and time-consuming because these lipophilic vitamins occur at low levels and are subject to matrix interference, particularly from phospholipids. The new method established here provides fast, accurate, and precise vitamin K levels in plasma using a simple phospholipid removal procedure and a sensitive 4-minute LC-MS/MS analysis on a Raptor Biphenyl column, making it suitable for high-throughput testing.Quantitative analysis of bile acids in plasma is critical for diagnosing liver disease as well as assessing drug safety. Accurate reporting can be difficult because of analyte characteristics, matrix effects, and other factors. Here, we establish a rapid, robust, and selective LC-MS/MS method for the analysis of bile acids in human plasma using a Restek Raptor C18 column. One of the significant improvements over other reported methods was the baseline separation of all 17 bile acids in 6 minutes including three isomer groups.

High-Throughput Analysis of Mycotoxins in Cannabis CBD Oil Pairs Simplified Cleanup with LC-MS/MS Sensitivity

Published By: Restek Corporation

Year of Publication: 2018

Link: http://www.restek.com/Technical-Resources/Technical-Library/Foods-Flavors-Fragrances/fff_FFSS2946-UNV

Abstract: Complex, time-consuming immunoaffinity column sample prep protocols for mycotoxins can be replaced by this simple pass-through SPE sample cleanup. Paired with LC-MS/MS analysis using a Raptor Biphenyl column, excellent chromatographic results were obtained for aflatoxins and ochratoxins at 2 ng/g, providing a high-throughput solution for analyzing mycotoxins in cannabis.