Remove Both Phospholipids and Proteins in One Resprep PLR SPE Procedure

Simultaneously remove phospholipids and proteins in a single, simple procedure with new Resprep PLR (phospholipid removal) SPE products. Whole blood, serum, and plasma all contain proteins and phospholipids that can interfere with target analytes and hasten the need for instrument maintenance. It’s important to remove them from samples prior to analysis to avoid signal suppression, and Resprep PLR SPE cartridges or 96-well plates make this an easy task by combining protein precipitation and phospholipid removal in one sample preparation process. No analyte-specific method development is required because the same procedure can be used for samples containing acids, bases, or neutral compounds. In addition, effective removal of phospholipids and proteins from sample extracts reduces contamination, minimizing the frequency of instrument maintenance.

Phospholipid and Protein Removal in One Simple SPE Process Prevents Matrix-Based Signal Suppression

Published By: Restek Corporation

Year of Publication: 2020


Abstract: Biological samples contain proteins and phospholipids that can interfere with LC-MS/MS analysis and increase the frequency of instrument maintenance. Here we used Resprep PLR SPE to simultaneously remove proteins and phospholipids from plasma in one high-efficiency sample preparation process. Concurrent phospholipid removal eliminated signal suppression and excellent recoveries were obtained for a wide range of chemical classes without the need for additional method development.