Liquid Chromatography: Pharmaceutical Applications (Chapter in Reference Module in Chemistry, Molecular Sciences and Chemical Engineering)

Author(s): D.M. Diehl1, David S. Bell2, Barbara Bojko3
1. Waters Corporation, 2. Restek Corporation, 3. Nicolaus Copernicus University

Published By: Elsevier

Year of Publication: 2018

Link: https://www.sciencedirect.com/science/article/pii/B9780124095472144920

Abstract: Liquid chromatography (LC) has been applied in the pharmaceutical industry almost from the inception of the technique. In this article, the overview of recent advancements in LC, like ultra-high-performance liquid chromatography and superficially porous particle (SPP) technology, which were adopted to analysis of pharmaceuticals can be found. The utilization of liquid chromatography during development and manufacturing stages of small molecule drugs is described. That includes aspects of purification, isolation, identification, and stability, among others. The article also considers application of modern LC strategies to analysis of the currently most emerging group of drugs, biopharmaceuticals. Finally, the LC approaches for metabolomics in drug discovery as well as pharmacometabolomics are discussed.

When Do We Need Sub2m Superficially Porous Particles for Liquid Chromatography Separations

Author(s): David Bell, Landon Wiest, Shun-Hsin Liang, Dan Li
Restek Corporation

Published By: LCGC Asia Pacific

Issue: Volume 21, Issue 3

Year of Publication: 2018

Link: http://www.chromatographyonline.com/when-do-we-need-sub2m-superficially-porous-particles-liquid-chromatography-separations

Abstract: The use of superficially porous particles (SPPs) for modern high performance liquid chromatography (HPLC) is now very common. Initially, SPPs rose as an alternative to sub-2-µm fully porous particles (FPPs). In recent years, many column manufacturers have developed 2-µm and smaller SPP-based products. This article investigates the practical utility of these smaller SPP designs.

When Do We Need Sub-2-µm Superficially Porous Particles for Liquid Chromatography Separations?

Authors: David S. Bell, Landon Wiest, Shun-Hsin Liang, Dan Li

Restek Corporation

Published By: LCGC North America

Year of Publication: July 2018

Volume, Issue: Volume 36, Issue 7

Link: http://www.chromatographyonline.com/when-do-we-need-sub-2-m-superficially-porous-particles-liquid-chromatography-separations-0

Abstract: The use of superficially porous particles (SPPs) for modern high performance liquid chromatography (HPLC) is now very common. Initially, SPPs rose as an alternative to sub-2-µm fully porous particles (FPPs). In recent years, many column manufacturers have developed 2-µm and smaller SPP-based products. This article investigates the practical utility of these smaller SPP designs.

Beyond Particle Technology

Author: David S. Bell

Restek Corporation

Published By: Supplement to LCGC North America

Year of Publication: June 2018

Volume, Number: Volume 36, Number s6

Link: http://files.pharmtech.com/alfresco_images/pharma/2018/06/13/f119cb54-ce43-4628-95f8-af1aa07bb6ff/LCGC_NAmerica_June2018Supp.pdf

Abstract: Two of the more significant technologies, sub-2-μm particles and superficially porous particles (SPP), have taken a firm hold on modern liquid chromatography practice. Each of these developments were initially met with both excitement and their share of skepticism. Both emotions drove extensive research and ultimately adoption of the ideas. Today, both technologies are routinely used in many industries around the world, but where do we go from here? This supplement was assembled to provide examples of the ongoing research that is building upon recent particle technology developments.

The Effects of LC Particle Choice on Column Performance: 2.7 vs. 5 µm Diameter Superficially Porous Particles (SPP)

Author(s): Sharon Lupo, Ty Kahler, and Paul Connolly
Restek Corporation

Published By: Restek Corporation

Year of Publication: 2014

Link: http://www.restek.com/Technical-Resources/Technical-Library/General-Interest/generic_GNAR2079-UNV

Abstract: Restek’s Raptor™ LC columns feature superficially porous particles (commonly referred to as SPP or “core-shell” particles) and are available in both 2.7 and 5 µm particle sizes, giving analysts greater flexibility. However, it may not always be clear which particle size to choose. In this technical note, we will examine the differences in efficiency, sensitivity, and pressure between Raptor™ LC columns packed with 2.7 vs. 5 µm particles and provide advice on making the appropriate LC particle choice.

Raptor™ Speed, Efficiency, and Ruggedness is Now Available in C18

Raptor C18 ColumnsEvery LC lab has a cache of C18s, but every C18 is not created equal. Because the chemistry tends to be similar, the silica support that carries this ubiquitous octadecylsilane phase becomes vitally important. When you need a general-purpose LC column, don’t just grab any C18. Choose the speed, efficiency, and long-lasting ruggedness of the new Raptor™ C18 SPP LC column.

Raptor™ C18 LigandThe traditional end-capped Raptor™ C18 offers the highest hydrophobic retention of any Raptor™ phase, and it is compatible with a wide range of mobile phases from moderately acidic to neutral (pH 2–8). Whether for food safety or environmental or bioanalytical analyses, this new phase offers consistently excellent data quality in less time across myriad reversed-phase applications, matrices, and compound classes.

To lower costs and improve profitability, you need columns to last longer, data to be reproducible, and existing HPLC instrumentation to run faster. Get there with the only general-purpose C18 that gives you Selectivity Accelerated.

www.restek.com/raptor

About Raptor™ SPP LC Columns:
Superficially porous particles (commonly referred to as SPP or “core-shell” particles) changed the world of LC by dramatically boosting column efficiency and reducing analysis times, but they were only the beginning. With Raptor™ LC columns, Restek chemists have combined the speed of SPP with the resolution of highly selective USLC® technology. This new species of chromatographic column allows you to more easily achieve peak separation and faster analysis times without expensive UHPLC instrumentation. Raptor™ LC columns provide the practicing analyst with the most powerful tools available for fast, efficient method development and increased sample throughput. And because they are from Restek, Raptor™ LC columns are backed by manufacturing and quality systems you trust along with internationally renowned Plus 1 service.

Breathe New Life Into Your Old HPLC With Restek’s 5 μm Raptor™ Columns

Raptor™ SPP LC ColumnsWith the advent of superficially porous particles (SPP or “core-shell” particles), the analytical community found the ability to gain far more efficiency and speed out of existing HPLC instrumentation. However, with these efficiency gains came higher pressures that exceeded the capabilities of many legacy HPLCs. To solve this issue, Restek has released rugged Raptor™ SPP core-shell columns with 5 µm particles.

The Raptor™ 5 μm particle provides all of the benefits of an SPP particle—better efficiency, peak shapes, signal-to-noise ratios, and sensitivity over fully porous particles of the same dimension—but without the significant increase in pressure. The improved efficiency and sensitivity of these 5 μm particles over 3 or 5 μm fully porous particle columns help you easily and significantly speed up existing methods on systems that simply cannot handle smaller 2.7 μm core-shell particles.

If you are looking to increase sample throughput and productivity on your existing 400-bar HPLC system, 5 μm Raptor™ columns are a perfect choice. Raptor™ SPP core-shell columns are available in 2.7 and now 5 μm particles with the time-tested Restek® Biphenyl or new acid-resistant ARC-18 phases. Additional phases are coming soon and will launch with both particle sizes.

Learn more about Raptor™ columns and experience Selectivity Accelerated at

www.restek.com/raptor

New Raptor™ ARC-18 Columns Are Born for LC-MS/MS

The birth of Restek’s new Raptor™ SPP LC column line began with the innovative Biphenyl phase, and it has now grown to include a new Restek® phase: the ARC-18.

Raptor™ LC ColumnsRaptor™ ARC-18 ColumnDesigned and intended specifically for use on LC-MS/MS systems, the Raptor™ ARC-18 column offers a well-balanced retention profile without the drawbacks of using an ordinary C18 in the harsh, acidic mobile phases needed for mass spectrometry (MS). Even after extended use in these low-pH (≤ 2.0) conditions, the sterically protected ARC-18 offers consistent retention, peak shape, and response for charged bases, neutral acids, small polar compounds, and more. For the rapid analysis of large, multiclass assays by LC-MS/MS, the Raptor™ ARC-18 truly is ahead of the curve.

www.restek.com/raptor

 

About Raptor™ SPP LC Columns: Superficially porous particles (commonly referred to as SPP or “core-shell” particles) changed the world of LC by dramatically boosting column efficiency and reducing analysis times, but they were only the beginning. With Raptor™ LC columns, Restek chemists have combined the speed of SPP with the resolution of highly selective USLC® technology. This new species of chromatographic column allows you to more easily achieve peak separation and faster analysis times without expensive UHPLC instrumentation. Raptor™ LC columns provide the practicing analyst with the most powerful tools available for fast, efficient method development and increased sample throughput. And because they are from Restek, Raptor™ LC columns are backed by manufacturing and quality systems you trust along with internationally renowned Plus 1 service.

Experience Selectivity Accelerated With New Raptor™ SPP LC Columns and Guards

Restek is excited to announce the evolution of superficially porous particles with the introduction of Raptor™ LC columns.

Raptor™ LC ColumnsSuperficially porous particles (commonly referred to as SPP or “core-shell” particles) changed the world of LC by dramatically boosting column efficiency and reducing analysis times, but they were only the beginning. With Raptor™ LC columns, Restek chemists have combined the speed of SPP with the resolution of highly selective USLC® technology. This new species of chromatographic column allows you to more easily achieve peak separation and faster analysis times without expensive UHPLC instrumentation. Raptor™ LC columns provide the practicing analyst with the most powerful tools available for fast, efficient method development and increased sample throughput. And because they are from Restek, Raptor™ LC columns are backed by manufacturing and quality systems you trust along with internationally renowned Plus 1 service.

Restek BiphenylThe first phase to be rolled out is the innovative Biphenyl, which will be followed by a full suite of selective USLC® phases. The industry-leading Biphenyl is Restek’s most popular LC stationary phase because it is particularly adept at separating compounds that are hard to resolve or that elute early on C18 and other phenyl chemistries. As a result, the rugged Raptor™ Biphenyl column is extremely useful for fast separations in bioanalytical testing applications like drug and metabolite analyses, especially those that require a mass spectrometer (MS). Increasing retention of early-eluting compounds can limit ionization suppression, and the heightened selectivity also helps eliminate the need for complex mobile phases that are not well suited for MS detection. Restek was the first to bring chemists the benefits of the Biphenyl ligand and has the knowledge to maximize SPP performance of this premier phenyl chemistry.

To help further extend the life of already-rugged Raptor™ LC columns, Restek has also mated these new superficially porous particles with patent-pending guard column hardware developed by Optimize TechnoloRaptor™ EXP® Guard Columnsgies. Sold separately and available in three different IDs, the Raptor™ LC guard column cartridge in an EXP® direct connect holder is the ultimate in column protection. Change cartridges without breaking inlet/outlet fluid connections—and without tools.

Experience Selectivity Accelerated. Put Raptor™ LC columns and guards to the test today on your most challenging workflows!

www.restek.com/raptor