Liquid Chromatography: Pharmaceutical Applications (Chapter in Reference Module in Chemistry, Molecular Sciences and Chemical Engineering)

Author(s): D.M. Diehl1, David S. Bell2, Barbara Bojko3
1. Waters Corporation, 2. Restek Corporation, 3. Nicolaus Copernicus University

Published By: Elsevier

Year of Publication: 2018

Link: https://www.sciencedirect.com/science/article/pii/B9780124095472144920

Abstract: Liquid chromatography (LC) has been applied in the pharmaceutical industry almost from the inception of the technique. In this article, the overview of recent advancements in LC, like ultra-high-performance liquid chromatography and superficially porous particle (SPP) technology, which were adopted to analysis of pharmaceuticals can be found. The utilization of liquid chromatography during development and manufacturing stages of small molecule drugs is described. That includes aspects of purification, isolation, identification, and stability, among others. The article also considers application of modern LC strategies to analysis of the currently most emerging group of drugs, biopharmaceuticals. Finally, the LC approaches for metabolomics in drug discovery as well as pharmacometabolomics are discussed.

When Do We Need Sub-2-µm Superficially Porous Particles for Liquid Chromatography Separations?

Authors: David S. Bell, Landon Wiest, Shun-Hsin Liang, Dan Li

Restek Corporation

Published By: LCGC North America

Year of Publication: July 2018

Volume, Issue: Volume 36, Issue 7

Link: http://www.chromatographyonline.com/when-do-we-need-sub-2-m-superficially-porous-particles-liquid-chromatography-separations-0

Abstract: The use of superficially porous particles (SPPs) for modern high performance liquid chromatography (HPLC) is now very common. Initially, SPPs rose as an alternative to sub-2-µm fully porous particles (FPPs). In recent years, many column manufacturers have developed 2-µm and smaller SPP-based products. This article investigates the practical utility of these smaller SPP designs.

Beyond Particle Technology

Author: David S. Bell

Restek Corporation

Published By: Supplement to LCGC North America

Year of Publication: June 2018

Volume, Number: Volume 36, Number s6

Link: http://files.pharmtech.com/alfresco_images/pharma/2018/06/13/f119cb54-ce43-4628-95f8-af1aa07bb6ff/LCGC_NAmerica_June2018Supp.pdf

Abstract: Two of the more significant technologies, sub-2-μm particles and superficially porous particles (SPP), have taken a firm hold on modern liquid chromatography practice. Each of these developments were initially met with both excitement and their share of skepticism. Both emotions drove extensive research and ultimately adoption of the ideas. Today, both technologies are routinely used in many industries around the world, but where do we go from here? This supplement was assembled to provide examples of the ongoing research that is building upon recent particle technology developments.

The Effects of LC Particle Choice on Column Performance: Switching from 3 and 5 µm Fully Porous Particles (FPP) to 5 µm Superficially Porous Particles (SPP)

Author(s): Sharon Lupo, Ty Kahler, and Paul Connolly
Restek Corporation

Published By: Restek Corporation

Year of Publication: 2014

Link:  http://www.restek.com/Technical-Resources/Technical-Library/General-Interest/general_GNAR2109-UNV

Abstract: In this technical note, we will compare the performance of Raptor™ 5 µm superficially porous particle (SPP) LC columns to traditional 3 and 5 µm fully porous particle (FPP) LC columns. We will demonstrate how faster, more efficient analyses can be attained with greater sensitivity and reduced system pressure by switching from FPP to SPP—without altering method conditions.

The Effects of LC Particle Choice on Column Performance: 2.7 vs. 5 µm Diameter Superficially Porous Particles (SPP)

Author(s): Sharon Lupo, Ty Kahler, and Paul Connolly
Restek Corporation

Published By: Restek Corporation

Year of Publication: 2014

Link: http://www.restek.com/Technical-Resources/Technical-Library/General-Interest/generic_GNAR2079-UNV

Abstract: Restek’s Raptor™ LC columns feature superficially porous particles (commonly referred to as SPP or “core-shell” particles) and are available in both 2.7 and 5 µm particle sizes, giving analysts greater flexibility. However, it may not always be clear which particle size to choose. In this technical note, we will examine the differences in efficiency, sensitivity, and pressure between Raptor™ LC columns packed with 2.7 vs. 5 µm particles and provide advice on making the appropriate LC particle choice.