Automated Inline Pigment Removal for the Analysis of Pesticide Residues in Spinach by Liquid Chromatography Tandem Mass Spectrometry

Author(s): Sharon A. Lupo, Randall L. Romesberg, Xiaoning Lu
Restek Corporation

Published By: Journal of Chromatography A

Year of Publication: 2020

Link: https://www.sciencedirect.com/science/article/pii/S0021967320307470

Abstract: An automated inline sample preparation (ILSP) method has been developed for pesticide residue analysis in spinach by LC-MS/MS. Chlorophyll pigments and other matrix constituents were removed from the sample extract using a UHPLC system equipped with an auxiliary pump, 6-port high pressure switching valve, and dual-directional ILSP cartridge containing bonded silica. The new procedure was evaluated as an entirely separate workflow using a simple solid-liquid extraction and as part of a cleanup strategy in conjunction with QuEChERS. Accuracy and precision experiments were conducted in spinach at two concentration levels (n=6). Of the 63 pesticides tested, 86% (0.005 mg/kg) and 100% (0.05 mg/kg) displayed average recoveries within 70-120% and RSD values ≤20% for the ILSP method. In addition, low to moderate matrix effects (<50%) were calculated for 95% of the analytes. Overall performance of the proposed method was found to be better or comparable to a traditional QuEChERS procedure utilizing AOAC formulated salts and dSPE sorbents, while significantly reducing the amount of pigments reaching the MS source. The ILSP workflow is a simpler procedure with fewer steps that require less time than traditional extraction and cleanup techniques.

New UHPLC Guard Cartridges Deliver Ultimate Protection for Restek’s 1.8 μm Raptor Columns

Raptor 1.8 μm LC columns unleashed superior Raptor performance on UHPLC analyses, and now Restek has released EXP UHPLC guard column cartridges made specifically for Raptor 1.8 μm columns.

These new guards provide extreme protection from particulates and matrix contamination, extend the lifetime of already rugged and long-lasting Raptor columns, and are proven to withstand the same UHPLC pressures as their analytical column counterparts. UltraShield filters are available for filtering out particulates, minimizing extra column volume, and maximizing sample throughput when using SPE, SLE, or other extensive sample preparations—but new Raptor EXP UHPLC guard column cartridges are an especially valuable alternative when using dilute-and-shoot or other minimal sample preparation techniques.

Further extend the life of your 1.8 μm Raptor UHPLC columns today at www.restek.com/catalog/view/40878

Restek Unleashes Superior Raptor Performance on Your UHPLC Analyses — Now with 1.8 μm Particles

Raptor LC columns have been providing higher efficiencies, faster analyses, and rock-solid reliability to HPLC users for several years.

And they have just evolved again.

Restek has brought the benefits of Raptor columns to those who have made the leap to UHPLC instruments, yet are still looking to boost their efficiencies further. Already sold with 2.7 and 5 μm superficially porous particles (SPP), Raptor columns are now available with 1.8 μm particles specifically for use with UHPLC. Choose from four distinctive Restek phase chemistries: Biphenyl, ARC-18, C18, and FluoroPhenyl.

Raptor 1.8 μm columns are designed and QC tested for maximum lifetime under UHPLC pressures. And, because they are Raptor SPP LC columns, they offer the peak capacity, selectivity, and reproducibility that analysts have learned to depend on from Restek.

Outfit your UHPLC with evolutionary Raptor 1.8 μm columns today at www.restek.com/raptor

About Raptor SPP LC Columns:
Superficially porous particles (commonly referred to as SPP or “core-shell” particles) changed the world of LC by dramatically boosting column efficiency and reducing analysis times, but they were only the beginning. With Raptor LC columns, Restek chemists have combined the speed of SPP with the resolution of highly selective USLC technology. This new species of chromatographic column allows you to more easily achieve peak separation and faster analysis times without expensive UHPLC instrumentation. Raptor LC columns provide the practicing analyst with the most powerful tools available for fast, efficient method development and increased sample throughput. And, because they are from Restek, Raptor LC columns are backed by manufacturing and quality systems you trust along with internationally renowned Plus 1 service.

The Effects of LC Particle Choice on Column Performance: Raptor 1.8 µm Superficially Porous Particles (SPP) for UHPLC Analyses

Published By: Restek Corporation

Year of Publication: 2017

Link: www.restek.com/Technical-Resources/Technical-Library/General-Interest/gen_GNAR2803-UNV

Abstract: Superficially porous particles (commonly referred to as SPP or “core-shell” particles) have been proven to provide fast and efficient LC separations. These particles feature a solid, impermeable core enveloped by a thin, porous layer of silica that offers significantly higher efficiency and sensitivity than traditional fully porous particles (FPP). At launch, Raptor SPP LC columns were touted for their selectivity and for their ability to help customers achieve faster separations with their existing HPLC instruments. With the launch of Raptor 1.8 µm columns, this superior Raptor performance has been unleashed on your UHPLC analyses. But, what exactly does that mean? In this technical note, we will explore the benefits of using Raptor 1.8 µm LC columns in your UHPLC instrument with a focus on peak capacity, column lifetime, and selectivity.

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Apply Force to Your LC Methods with Restek’s New High-Performance Columns

Force LC ColumnsRestek’s new Force performance LC columns give you the power to maximize instrument uptime, increase productivity, and transfer methods across your entire lab,  from your trusted-but-aged HPLC to the cutting-edge UHPLC you just plumbed last week. This incredibly rugged and supremely consistent column line represents the culmination of Restek’s twenty-plus years in LC dedicated to continually improving phase chemistries, lot and lifetime testing, and bonding and packing procedures.

Long-lasting and reproducible, new Force columns maintain stable retention times and peak shapes, even under the stress of elevated UHPLC pressures and rapid cycling. Premium quality is ensured by strict manufacturing and QC procedures—and backed by the strength of the Restek 100% Pure Satisfaction guarantee. And perhaps most important, Force premium LC columns are fully scalable between 3 or 5 μm HPLC and 1.8 μm UHPLC. You can easily transfer between instrument platforms and optimize methods without extensive calculations.

Available in three particle sizes, Force columns feature Restek’s most-popular and highly selective Biphenyl and FluoroPhenyl phases, as well as a general-purpose C18. Meet today’s workflow needs—and prepare for tomorrow’s—by applying Force LC columns to all of your instrument platforms.

Learn more and order today at www.restek.com/force