Understanding Selectivity in Reversed Phase Separations – A Simplified Approach to HPLC and UHPLC Column Selection

Author(s): Rick Lake, Ty Kahler

Published By: Restek Corporation

Year of Publication: 2011

Link: http://www.restek.com/Technical-Resources/Technical-Library/Pharmaceutical/general_PHAN1348

Abstract: Column selectivity has the most significant influence on chromatographic peak separation, or resolution, so choosing the right column can greatly speed up HPLC and UHPLC method development. In this article, we discuss column choice and identify a set of just 4 stationary phases—Restek’s USLC™ column set—that encompasses the widest range of reversed phase selectivity available today.

The New USLC® Toolbox Gives You All the Right Tools—All in One Box

USLC™ Method Development ToolboxRestek’s USLC® column set represents the widest range of reversed phase selectivity available with just 4 stationary phases: Aqueous C18, IBD, Biphenyl, and PFP Propyl. It simplifies column choice for fast, effective method development—and new USLC® toolboxes make things even easier!

A USLC® method development toolbox contains all 4 USLC® stationary phases in 1 convenient package. Available for UHPLC (1.9 µm) and HPLC (3 or 5 µm) in 50, 100, or 150 mm lengths, this must-have companion for method developers also includes a selection guide to help ensure that you always choose the right column the first time.

To learn more about USLC® technology and order your lab a method development toolbox, call 1-814-353-1300, ext. 3, or visit www.restek.com/uslc today.

Restek USLC® Columns: Choose Columns Fast. Develop Methods Faster.