Cannabidivarin (CBDV) Added to Restek’s Selection of Cannabis Reference Standards

Cannabis strains with high levels of cannabidiol (CBD) typically have higher levels of cannabidivarin (CBDV) as well, but accurate quantitation of both compounds for potency and strain ID is a must, especially as interest in using CBDV as an anticonvulsant and anti-nausea treatment continues to rise. Restek now offers a stand-alone CBDV reference standard—in addition to CBD and a host of other compounds—to help labs with the comprehensive analysis of cannabis products.

Restek’s CBDV standard is ideal for creating multipoint (5-point minimum suggested) calibration curves for LC-MS/MS. With verified composition and stability, this prepared stock product eliminates the need for in-house standards preparation for greater convenience and lower labor requirements. As a U.S. DEA-exempted formulation, no additional customer permits or licensing are required to purchase within the U.S., and because it’s manufactured and QC tested in Restek’s ISO-accredited labs, this new CBDV standard qualifies as a certified reference material (CRM) that satisfies your ISO requirements.

For CBDV, CBD, and other cannabis reference standards, along with the expertise and consumables your lab needs, turn to www.restek.com/cannabis

Quickly and Efficiently Extract Both Volatiles and Semivolatiles with New Triple-Phase SPME Fibers and Arrows

Restek first launched solid phase microextraction (SPME) automated sample preparation fibers to reduce sample handling and solvent consumption in many industries, such as environmental, food, and clinical. Soon after came rugged Restek PAL SPME Arrows, a revolution in microextraction that combines exceptional robustness with faster extraction times and trace-level sensitivity. Now, both fiber and Arrow lines are being expanded, including the addition of a sought-after triple-phase offering.

Of significant interest to many analysts, new triple-phase SPME fibers and Arrows provide exceptional versatility. This divinylbenzene (DVB)/carbon wide range (WR)/polydimethylsiloxane (PDMS) combination can be used for quick and efficient extraction of a wide range of both volatiles and semivolatiles, and it is an excellent choice for decision-making during method development because it combines the characteristics of three popular phases.

Restek’s full SPME line of fibers, Arrows, and accessories can be found at www.restek.com/SPME

New Pesticide CRMs Released Specifically for Cannabis Labs in California and Similar States

Restek’s support for cannabis-testing labs continues to grow. This latest offering will help you meet the specific cannabis analysis needs of California set forth by the Bureau of Cannabis Control for regulated category I and II residual pesticide reporting—and of other states with similar regulations/programs. Ideal for creating multipoint (5-point minimum suggested) calibration curves for GC- and LC-MS/MS, these six mixes of prepared stock standards also eliminate the need for in-house standards preparation. And, as with all certified reference materials (CRMs) manufactured and QC tested in Restek’s ISO-accredited labs, they will satisfy your ISO requirements.

Order today at www.restek.com/cannabis

Differential metabolic signatures in naturally and lactic acid bacteria (LAB) fermented ting (a Southern African food) with different tannin content, as revealed by gas chromatography mass spectrometry (GC–MS)-based metabolomics

Author(s): Oluwafemi Ayodeji Adebo1, Eugenie Kayitesi1, Fidele Tugizimana2,3, Patrick Berka Njobeh1

1. Department of Biotechnology and Food Technology, University of Johannesburg

2. Research Centre for Plant Metabolomics, Department of Biochemistry, University of Johannesburg

3. International R&D, Omnia Group, Ltd

Published By: Food Research International

Issue: Vol. 121, July 2019

Year of Publication: 2019

Link: https://www.sciencedirect.com/science/article/pii/S0963996919302017

Abstract: Fermented whole grain (WG) sorghum food products including WG-ting can be obtained from different sample sources and fermentation conditions, leading subsequently to variations in the molecular composition of the products. There is however, a lack of detailed understanding and description of differential molecular profiles of these food products. Thus, the current study is a nontargeted gas chromatography-mass spectrometry (GC–MS)-based metabolomics approach to descriptively elucidate metabolic profiles of two WG-sorghum types [high tannin (HT) and low tannin (LT)] and their derived WG-ting products obtained via fermentation. Metabolites were extracted with 80% aqueous methanol and analyzed on a gas chromatography high resolution time of flight mass spectrometry (GC-HRTOF-MS) system. Chemometric methods such as principal component analysis (PCA) and orthogonal partial least square-discriminant analysis (OPLS-DA) were applied to mine the generated data. Our results showed that tannin contents influenced the composition of the raw sorghum and derived WG-ting samples. Metabolite signatures that differentiated raw HT- and LT-sorghum included cyclic compounds, pesticides, 2,4-di-tert-butylphenol, fatty acid esters, and sugar derivatives. Furthermore, fermentation of the HT- and LT-sorghum into WG-ting led to an increase in the levels of fatty acids, fatty acid esters and some other compounds which are vital from a dietary and health context. Equally observed were reduction of some phenols, cyclic compounds, a pesticide and ketone. Thus, the results demonstrated that the inherent metabolic composition of raw sorghum would lead to differential metabolic changes in the fermented products such as WG-ting, with subsequent dietary and health implications. Fermenting ting with Lactobacillus fermentum FUA 3321 was most desirable as relevant metabolites were observed in both HT- and LT-ting samples. Furthermore, the study highlights the applicability of GC–MS metabolomics in understanding WG-ting fermentation.

Note(s): The study was done using the Rxi-5ms column.

Quantitation of Mycotoxins in Four Food Matrices Comparing Stable Isotope Dilution Assay (SIDA) with Matrix-Matched Calibration Methods by LC–MS/MS

Author(s): Dan Li; Justin A. Steimling; Joseph D. Konschnik, Scott L. Grossman; Ty W. Kahler
Restek Corporation

Published By: Journal of AOAC International

Year of Publication: 2019

Link: https://www.ingentaconnect.com/content/aoac/jaoac/pre-prints/content-jaoac_190028

Abstract: Background: Mycotoxins are big concerns in food safety. Analytical methods are important for the evaluation of mycotoxins in different food commodities. Objective: In this study, stable isotope dilution assay (SIDA) was compared with a matrix-matched calibration method for the quantification of mycotoxins in four different commercially available commodities and two reference materials. Methods: All samples were extracted with water–acetonitrile (50+50, v/v), followed by filtration and LC–tandem MS analysis. Results: SIDA calibration accuracies ranged from 78.6 to 112% with relative SDs (RSDs) ≤16% across all four matrices. The majority of the recoveries across all matrices ranged from 70 to 120% with RSDs <11%. Of the four mycotoxins in the reference materials analyzed, only three had 13C-internal standard (IS), whereas the fourth was quantified using a closely eluting 13C-IS for a different mycotoxin. Mycotoxins paired with their corresponding 13C-IS had accuracies >90%, whereas accuracies for the mismatched mycotoxin/13C-IS were <14%. Conclusions: When 13C-IS are not available, matrix-matched calibration was also evaluated as an alternative to quantitating target mycotoxins. The use of 13C-IS is the best way to dynamically account for prevalent matrix effects, but matrix matching provides a viable alternative. Highlights: The study compared SIDA and matrix-matched calibration methods in terms of recovery, efficiency, advantages, and limitations for LC-MS based mycotoxin analysis

New CBGA Reference Standard for Your Cannabis Potency Testing Program

Restek’s ever-growing selection of cannabis reference standards has expanded again with a new cannabigerolic acid (CBGA) CRM. This certified reference material (CRM) is U.S. DEA exempt, so there’s no need to purchase costly neat materials, make your own standards, or deal with DEA forms and registrations. Verified in composition and stability, this CBGA standard is ideal for combining with other Restek cannabinoid CRMs for quantitative potency analysis using the Raptor ARC-18 LC column. And like all Restek certified reference materials (CRMs), it is manufactured and QC tested in ISO-accredited labs to satisfy your ISO requirements.

Your CBGA standard is in stock now at www.restek.com/catalog/view/54064 You can also find it, along with the rest of Restek’s cannabis analysis solutions, at www.restek.com/cannabis