Association of Official Analytical Chemists (AOAC 2023)
25-30 August, New Orleans, LA, U.S.
Visit the AOAC 2023 website
Learn More About Featured Restek Products
- Pro EZLC Method Translator
- Cannabinoids Acids 7 Standard
- Cannabinoids Neutrals 9 Standard
- Raptor Biphenyl LC Columns and Guards
- Raptor ARC-18 HPLC Columns
- Force Biphenyl LC Columns
- Ultra IBD Columns and Guards
- Low-Pressure GC (LPGC) Column Kit
Questions about these presentations? Contact Restek Technical Service.
RESTEK TECHNICAL POSTERS:
Large Volume Injection of Pesticides in Matrix on Low-Pressure Gas Chromatography
Jessi Collier (presenter), Jana Hepner, Jaap de Zeeuw, Joe Konschnik, Chris English
Restek Corporation
Concurrent Solvent Recondensation Large Sample Volume Splitless Injection (CSR-LVSI, or LVI) is a gas chromatography sample injection technique that overcomes the limitation of using a smaller sample volume of 1to 2 μL, which is typical of conventional splitless injections. Low-Pressure Gas Chromatography (LPGC) is a novel technique that has been successfully applied for multiresidue pesticide detection and quantification. The LPGC configuration with the restrictor/guard column facilitates the requirements of a CSR-LVSI and has a potential to improve the sensitivity and lower detection limits. Peak shapes of large volume injections using both acetonitrile and acetonitrile-toluene were evaluated in the range of 5–50 μL, and the relationship between the peak area and injection volume was established. The calibration parameters were compared for 5, 15, and 25 μL injection insolvent and matrix. The limits of detection and other parameters were compared between the two injection volumes.
Measuring Stability of Combined Neutral and Acid Cannabinoids for Instrument Calibration
Kendra Jozefick (presenter), Casey Guyer, Dan DeLurio, Jason Fisher, Gary Stidsen
Restek Corporation
There are several different comprehensive analytical reference materials available on the market that target various neutral and acidic cannabinoids for the analysis of cannabis commodities. The key to a successful analysis is starting with standards that are ISO-accredited, have strict environmental controls, verified purity, and stringent mix stability testing. Our first study tested the stability of individual mixes that have been stored at 0°C, 10°C, and 25°C for 28 days in comparison to the control standards that were stored at -20°C. The second part of the study tested the combined stability of the neutral cannabinoids mixed with the acidic cannabinoid standards stored at room temperature for 20 days. Data of all test results will be shown.
Cannabis Potency Testing—Which Column Dimension is Right for You?
Dan DeLurio (presenter)
Restek Corporation
When starting method development for potency testing, it’s important to choose the right column dimension for the target analysis. In this work, different column dimensions of the Raptor ARC-18 phase were utilized to develop methods to meet various labs’ needs using HPLC-UV. To demonstrate the powerful resolving capabilities of Raptor ARC-18, a 50 x 3 mm, 2.7 µm column was used to analyze 7 cannabinoids including CBD, CBDA, delta-9-THC, delta-8-THC, (6aR, 9S)-delta-10-THC, (6aR, 9R)-delta-10-THC, and THCA. Next, additional cannabinoids including CBDV, THCV, CBG, CBN, CBGA, and CBC were added to the previous analytes for a total of 13 cannabinoids. Using the same column dimension and mobile phases, a method was developed to resolve all analytes in 10 minutes. Finally, to include exo-THC and CBNA, a 150 x 3 mm, 2.7 µm column dimension was used to demonstrate the utility of a longer column dimension. Each of these methods was applied to hemp matrix to demonstrate the applicability of these methods in real-world samples.
A Virtual Liquid Chromatography Method Development Tool for Cannabinoid Analysis
Dan DeLurio (presenter)
Restek Corporation
The development and optimization of cannabinoid separations can be time-consuming and costly, often requiring several steps including method scouting, development, and optimization. To reduce cost and save time, an instrument-free software modeling tool was developed. This allows users to select compounds from a database and instantly model a separation by adjusting parameters, such as instrument/system effects (dwell and extra column volume), temperature, and mobile phase additives. The modeler delivers a fast, no-cost starting point for LC method developers, novice, and expert, who either lack the expertise, or the time, to develop and optimize separations quickly and accurately. This novel, virtual method development software can improve turnaround time, increase throughput to existing methods, and offer an on-demand consultative user experience.
A Streamlined Cost-Effective Cannabis and Hemp Flower Workflow for Potency Testing
Dan DeLurio (presenter)
Restek Corporation
As the cannabis industry sees continued growth, production labs are looking to improve sample throughput and cut costs per sample for potency analysis. Due to ever-changing rules and regulations, it can become difficult to improve existing workflows. Keeping high-throughput labs in mind, a study was completed to obtain a streamline workflow with minimal process time, sample touch time, and keeping the cost of sample prep materials at a minimum. The study was completed by comparing different extraction volumes, vortex times, and using a filter mate versus a centrifuge. A total of 16 cannabinoids were monitored on a UV-vis at 228 nm using a Raptor ARC-18 150 x 4.6 mm 2.7 µm analytical column with an accompanying 5 x 4.6 mm EXP guard under isocratic conditions with a total cycle time of 10 minutes.
The HPLC- UV Analysis of Psilocin, Psilocybin, and Others Found in Psychedelic Compound Containing Mushrooms and Infused Products
Dan DeLurio (presenter)
Restek Corporation
Psilocin and psilocybin are tryptamine alkaloids found in some plants and fungi. Often found in certain species of mushrooms, these compounds are used for their psychotropic effects, but microdosing has shown promise for the treatment of anxiety, depression, and other mental health disorders. With recent decriminalization and legalization in certain areas, of both naturally derived and synthetic psychedelic compounds, labs are seeking to begin potency testing of psychedelic compound containing mushrooms and infused products. A set of efficient methods were developed for the testing of two psychedelic compound containing mushroom species. Sample preparation and extraction processes were established for infused chocolates, gummies, and extracts, as well as freshly ground mushrooms. Due to the robust nature, an HPLC-UV system was used to collect all data. A method for six compounds endogenous to psychedelic compound containing mushrooms was developed using a Force Biphenyl 100 x 3.0 mm, 3 μm column with a full cycle time of 7.5 minutes. Another method for the analysis of psychedelic compounds endogenous to the Amanita muscaria mushroom, ibotenic acid, and muscimol was developed utilizing an Ultra IBD 30 x 3.0 mm, 3 μm column with a 4.5-minute cycle time.