Restek at Forum Labo 2025

25-27 March
Booth C073
Paris, France

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VENDOR SEMINAR

Une solution pour développer, optimiser ou transposer une méthode en GC et LC : les modélisateurs gratuits Pro EZGC et Pro EZLC

Thierry Laurent, Chaza Chbib (presenters)
Restek Corporation
Tuesday, March 25, 2:00 p.m.

Abstract
Dans cette présentation, nous décrirons le fonctionnement de notre modélisateur de chromatogrammes Pro EZGC (chromatographie gaz), un outil puissant qui permet de réaliser des transpositions de méthodes (changement de gaz vecteur, modification des dimensions de la colonne, du type de détection, etc.)

Le logiciel permet aussi et surtout de travailler en amont des analyses sur les développements de méthodes complets : choix de la phase stationnaire, paramétrage des conditions d’injection, gradient de température, débits, composés à séparer, optimisation des résolutions ainsi que les temps d’analyse.

Nous évoquerons également son équivalent en chromatographie en phase liquide, le Pro EZLC pour le développement de méthode et l’optimisation en LC-MS/MS.
Dans le cadre de l’analyse des PFAS, nitrosamines, cannabinoïdes, et autres substances psychoactives, cet outil permet de choisir la colonne ainsi que les conditions analytiques appropriées, sans l’aide d’instrument.

POSTER PRESENTATIONS

Incorporating Ultrashort-Chain Compounds into the Comprehensive Analysis of PFAS in Potable and Non-Potable Waters

Shun-Hsin Liang, Justin Steimling (authors)
Restek Corporation

Abstract
Ultrashort-chain (USC) per- and polyfluoroalkyl substances (PFAS) are small and very polar compounds with carbon chain lengths shorter than C4. Their ubiquitous and high levels of occurrence in environmental aquatic systems are emerging as a significant concern, rivaling the well-established issues associated with long-chain PFAS contamination. Therefore, it is important to analyze both USC and long-chain PFAS together in water samples to comprehensively assess and address the full spectrum of PFAS contamination. The high polarity of USC PFAS poses a challenge for standard chromatographic practices in PFAS analysis, primarily due to insufficient chromatographic retention.

In this study, a simple and reliable workflow was developed for the simultaneous analysis of C1 to C14 perfluoroalkyl carboxylic and sulfonic acids, along with other groups of PFAS, in both potable and non-potable waters. The chromatographic separation was conducted using a polar-embedded, reversed-phase LC column with an inert coating on the hardware. Three different water matrices (tap water, bottled water, sewage treatment wastewater) were chosen for method evaluation to demonstrate the applicability of the developed workflow for measuring 45 PFAS compounds in diverse water samples. A dilute-and-shoot workflow was evaluated by accuracy and precision analysis at five fortification levels ranging from 2 to 250 ng/L. Eighteen isotopically labeled PFAS, serving as extracted internal standards, were added to the samples at 100 ng/L to validate the accuracy of the entire workflow. Calibration standards were prepared in reverse osmosis water due to its cleanliness for all analytes. The calibration ranges varied among different analytes, spanning from 1–1000 ng/L.

All analytes and extracted internal standards exhibited recovery values ranging from 70% to 130% of the nominal concentration across all fortification levels. Satisfactory method precision was demonstrated with %RSD values below 20%. Additional potable and non-potable waters collected from various source waters were tested to further demonstrate that the established workflow is suitable for the accurate quantification of targeted PFAS in a wide range of water matrices.

Development of a Semivolatile Column Optimized for the Analysis of Hazardous Waste

Chris English, Corby Hilliard, Christopher Rattray, Kristi Sellers, Whitney Dudek-Salisbury, Mike Chang, Brian Salisbury (authors)
Restek Corporation

Abstract
Analyzing basic, neutral, and acidic semivolatile environmental pollutants, such as those found in U.S. EPA 8270, at increasingly lower levels of detection puts demands on the entire analytical system. One of the greatest challenges is maintaining system performance following repeated injections of highly contaminated samples. The goal is to develop a column tuned for PAH selectivity while maintaining the same elution profile labs expect from other silarylene “5-type” columns along with improved lifetime.

This presentation will review the details of the lifetime study results using contaminated extracts and will highlight the advantages of this column using a new deactivation and stationary phase.

EVOT4440-UNV