EPRW 2022

Vendor Seminar
New Techniques to Simplify the Analysis of Pesticides: A New Hybrid Phase for Mini-Screenings of Very Polar Analytes and In-Line Sample Preparation for a Variety of Sample Matrices.
For some time now, polar contaminants have been experiencing increased interest because they are not easily accessible with existing analytical methods, such as GC or reversed-phase LC. Polar pesticides, for example, cannot be included into the large panels for screening methods in food safety analysis.
Various suppliers have now successfully applied novel mixed-mode separation phases to the analysis of polar compounds like polar pesticides or polar, short-chain PFAS.
In this vendor seminar, Dr. Emanuele Ceccon will show how such a mixed-mode column can be applied in mini-screenings for even larger groups of similar components.
Up to 17 polar pesticides, including glyphosate, can be detected in one run, which has led to a method based on this column recently being included in the QuPPe document of the European Reference Laboratory for Single Residue Analysis.
Sample preparation stays a key in pesticide analysis. In the past, QuEChERS, invented by Michelangelo Anastasiadis and Steve Lahotey, has shown to be a good compromise to extract compounds of interest from difficult matrices like food commodities.
Nevertheless, QuEChERS stays difficult to be automized. In the second part of this vendor seminar, Dr. Emanuele Ceccon will report about an in-line sample prep (ILSP) approach that provides a semiautomated cleanup procedure for the analysis of pesticide residues in food by LC-MS/MS. ILSP selectively retains matrix components from the sample extract and can be utilized as a standalone workflow or integrated into an existing QuEChERS workflow.
Following this vendor seminar, Restek invites you to stay for a typical Italian aperitivo for a refreshing start to the conference.
- Information:
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16:35 - 17:0019 September 2022
- Authors:
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- Emanuele Ceccon
- Affiliations:
- Restek Corporation
- Presenters:
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Emanuele Ceccon
Poster Presentation
GC-MS/MS 200+ Multiresidue Pesticide Screening Workflow—Comparison of Conventional 30 m Column and LPGC Kit
LC-MS/MS Analysis of Glyphosate and Polar Contaminants in Food Using a Hybrid Ion-Exchange/HILIC Column
The safety residual level of glyphosate in food is regulated in the U.S., Europe, and other global regions. Direct analysis of underivatized glyphosate, however, can be challenging due to its minimum retention on a reversed-phase or hydrophilic interaction chromatography (HILIC) column and severe adsorption onto the stainless flow path. On the other hand, glyphosate and other polar anionic contaminants tend to strongly retain on an anion-exchange column and require high salt mobile phase to elute, which is not friendly to mass spectrometric analysis.
These challenges motivated the invention of a hybrid ion-exchange/HILIC column offering balanced retention and separation of ionic and other highly polar compounds. An LC-MS/MS solution was developed for the direct analysis of underivatized glyphosate, aminomethylphosphonic acid (AMPA), and glufosinate, as well as other 14 highly polar contaminants listed in the Quick Polar Pesticides Extraction (QuPPe) method in seven food matrices (rice, baby cereal, soybean, lemon grass, oregano, tea, and strawberry). All 17 compounds were analyzed simultaneously in a single run with a total analysis time of less than 19 minutes. This method was able to chromatographically separate components sharing the same mass fragments, including AMPA/fosetyl aluminum and fosetyl aluminum/phosphonic acid. This approach enabled by unique column selectivity allows for the analysis of multiple polar pesticides in a simple method suitable for routine analysis.
Semiautomated Method for In-Line Removal of Matrix Components from Food for the Analysis of Residual Pesticides by LC-MS/MS