Restek at HPLC 2023
18-22 June, Duesseldorf, Germany
Visit the HPLC 2023 website
Learn More About Featured Restek Products
- Pro EZLC Chromatogram Modeler
- EPA 533 PFAS Calibration Standard
- EPA 537.1 PFAS Calibration Standard
- PFAS 24 Calibration Standard
- Raptor HILIC-Si LC Columns
- PFAS Delay Column
Questions about these presentations? Contact Restek Technical Service.
Visit us in booth 8 to use our Pro EZLC Chromatogram Modeler and to pick up a “Love Biphenyl“ T-shirt. And, also stop by 20 June at 4:30 PM for an after-hours “Beer with Restek.”
Vendor Seminar
The Development and Usage of a Virtual Liquid Chromatography Method Development Tool
20 June 2023, 1:30 p.m. - 2:00 p.m., Room 4 a+b
Authors: Cyrille Lamboley, Melinda Urich
Presenters: Cyrille Lamboley
The development and optimization of Liquid Chromatography (LC) separations can be time-consuming and costly, often requiring a number of steps including literature research, column selection, method scouting, method development, and method optimization. In an effort to eliminate these steps, an instrument-free, software modeling tool was developed that gives users the ability to select compounds from a database and instantly model a separation on different column phases.
Optimization of the chromatogram model can be performed while maintaining critical pair separations by adjusting parameters, such as instrument/system effects (e.g., dwell volume and extra column volume), mobile phase preferences, number of gradient steps, and more. The first version contains an initial library of approximately 250 Drugs of Abuse (DoA) with plans to expand by adding additional compounds, more libraries, and even more utility.
The modeler delivers a fast, no-cost starting point for LC method developers, novice, and expert, who either lack the expertise, or the time, to develop or optimize separations quickly and accurately. This novel, virtual method development software can improve turnaround time, increase throughput to existing methods, and offer an on-demand consultative user experience.
In this seminar, you will learn how to use Restek’s free Pro EZLC Chromatogram Modeler in order to develop and optimize LC-MS/MS methods before even setting foot in the lab.
Oral Presentation
Toward Green and Sustainable Hydrophilic Interaction Liquid Chromatography (HILIC) Workflows: Can We Replace Acetonitrile? Are There Alternative Modes?
21 June 2023, 5:30 p.m. - 5:45 p.m., Room 1 (Auditorium)
Authors: David Bell, Jan Pschierer, Sandra Ruiz Perez
Presenters: David Bell
The drive toward green and sustainable chemistry has increased significantly over the past several years. More recently, an increased focus on “greening” analytical workflows has been observed. From sample preparation to detection, every step must be critically evaluated to conserve energy and minimize environmental impact and waste.
Hydrophilic interaction liquid chromatography (HILIC) often utilizes a high percentage of acetonitrile in the mobile phase. Although acetonitrile is not the worst solvent in terms of sustainability, other solvents are considered to be more environmentally friendly, less toxic, and renewable. In this presentation we report on our efforts to replace or reduce acetonitrile usage in HILIC mode with more sustainable solvent systems. The results of direct acetonitrile replacement by various solvents are presented along with a variety of additional approaches, such as cosolvent implementation, to minimize acetonitrile where the usage of green solvents alone generated poor performance. In addition, alternative modes of chromatography are also discussed that may provide the retention and selectivity offered by HILIC, but in a more sustainable manner. Throughout the effort, underlying retention mechanisms are monitored and interpreted for greater overall chromatographic insight.
Poster Presentation
The Development of a Virtual Liquid Chromatography Method Development Tool
19 June 2023, 9:40 a.m. - 10:30 a.m., 1st Floor, Rooms 5,6,7,8
Authors: Diego Lopez1, Melinda Urich1, Jamie York1, Chris Nelson1, Justin Steimling1; John Garrett2
1. Restek Corporation, 2. Analytical Innovations, Inc.
Presenters: Diego Lopez
The development and optimization of liquid chromatography (LC) separations can be time-consuming and costly, often requiring a number of steps, including literature research, column selection, method scouting, method development, and method optimization. In an effort to eliminate these steps, an instrument-free, software modeling tool that gives users the ability to select compounds from a database and instantly model a separation on different column phases was developed.
Optimization of the model can be performed while maintaining critical pair separations by adjusting for instrument/system effects (e.g., dwell volume and extra column volume), mobile phase preferences, number of gradient steps, and more. The initial database consists of a Drugs of Abuse (DoA) library containing approximately 250 compounds with plans to continually expand the utility.
A modeled chromatogram and instrument-ready conditions are automatically generated and can be further optimized by users. During software development, the acceptance criteria for retention time agreement between wet-lab and modeled values was set at +/- 15 seconds. This range was chosen because it represents a typical MRM window. In the most complex portion of the verification, 704 retention time data points were collected in total for the 25 compounds used in the evaluation. Only 13 data points exceeded the+/- 15 second window with no compounds missing acceptance criteria by more than five seconds, giving an overall pass rate of 98.2%.
For LC method developers, novices, and experts who either lack the expertise, or the time, to develop separations quickly and accurately, this free tool can be used to deliver a fast, no-cost starting point for method development and optimization. This novel, virtual method development software can improve turnaround time, increase throughput to existing methods, and offer an on-demand consultative user experience.
Towards Greener Workflows with Clever Method Development
19 June 2023, 3:30 p.m. - 4:30 p.m., 1st Floor, Rooms 5,6,7,8
Authors: Sandra Ruiz Perez, Emanuele Ceccon, David Bell, Paul Connolly
Presenters: Sandra Ruiz Perez
Greener workflows can be accomplished by various parameters: working more energy efficiently, using less or no organic and toxic solvents, choosing more environmentally friendly solvents, trying to use renewable resources, reducing sample treatment, or using multi-methods. In a multi-method, more components can be analyzed in one run, which not only saves money but also minimizes organic waste and energy costs. Several approaches may be easily implemented in each lab without extra costs. Getting into green analytics, entrenched habits can sometimes make our work harder and more expensive.
Already, during method development, valuable instrument uptime, energy, organic solvents, and waste can be saved using no-cost virtual method development tools called “Pro EZLC Chromatogram Modeler” and “Pro EZLC Method Translator.” Both utilities save time, energy, and instrument uptime as you can develop or adapt your method without setting foot in the lab. Additionally, with the Pro EZLC Method Translator, you can check how to modernize your old methods (by using, e.g., a smaller inner diameter of your analytical column), maintain elution orders, and simultaneously see how much organic waste you save.
The development of a multimethod is challenging, but it can save instrument uptime and minimize organic waste and energy costs. The more components that are to be analyzed in one run, the more functionalities and reactivities come into play. Nowadays, also polar components become increasingly important. The right selectivity is crucial for success. As the C18 and all its variations (polar-embedded, sterically hindered, polymeric, etc.) are slowly reaching their limits, an alternative and more versatile stationary phase is gaining more and more attention. The Biphenyl separation phase retains an enhanced range of analytes via π, π- and hydrophobic interactions and is therefore perfectly suited for multimethods, as will be shown with several examples. Furthermore, we will show that various methods previously done with different kinds of C18 columns can all be realized on one Biphenyl column, which minimizes errors in daily routine and can reduce material costs. Towards the goal of a greener workflow in analytical chemistry, every small step done in every lab can help to save our environment.
The Evolving Landscape of PFAS Detection: An Outline of Methods
21 June 2023, 9:40 a.m. - 10:40 a.m., 1st Floor, Rooms 5,6,7,8
Authors: Dr. Jamie York, Dr. Shun-Hsin Liang
Presenters: Dr. Jamie York
Perfluoroalkyl substances (PFAS) are a group of man-made chemicals widely used in industrial applications and consumer products. Their widespread usage and resistance to degradation has resulted in PFAS being a ubiquitous environmental contaminant ,and the potential health effects is of growing concern. While many of the long-chain PFAS have been recognized as harmful, alternative compounds have emerged in their place. Short-chain PFAS compounds are considered to be less bioaccumulative and toxic than long-chain PFAS, but their widespread use has resulted in their increased environmental accumulation. In this work, several methods will be outlined to meet the evolving landscape of PFAS analysis. These methods include EPA methods 1633, 533, 8327, and 537.1 as well as a method for the analysis of ultra-short through short-chain (C1-C4) PFAS using a unique hybrid HILIC/ion-exchange stationary phase.