Restek at MSACL 2022

Direct oral anticoagulants (DOACs) have been increasingly prescribed for stroke prevention and treatment of thromboembolic disease (deep vein thrombosis and pulmonary embolism). An advantage of DOACs over other anticoagulants, such as warfarin, is that routine laboratory monitoring is not recommended. Despite the ease of dosing, DOACs introduce a risk for hemorrhages and life-threatening bleeding in high-risk patient populations. Laboratory testing may be useful for ruling out DOACs as a cause of major bleeds or in guiding the administration of DOAC reversal agents in patients with DOAC concentrations that exceed recommended levels. Unfortunately, standard coagulation assays, including prothrombin time (PT) and activated partial thromboplastin time (aPTT), correlate poorly with plasma concentrations of DOACs. The chromogenic anti-factor Xa assay is a reliable predictor of DOAC concentration; however, the utility of this test in emergency settings is limited by turnaround times of approximately one hour, heparin interference, and the inability to screen for multiple DOACs in one test.

Coated blade spray (CBS) is a solid-phase, microextraction-based technology that enables rapid qualitative and quantitative analysis of complex matrices through direct coupling with a mass spectrometer. CBS mass spectrometry (CBS-MS) has the potential to address the unmet clinical need of rapidly assessing and managing life-threating bleeding caused by DOACs. A quantitative CBS-MS assay was developed and validated to measure clinically relevant plasma concentrations of a panel of anticoagulants, including three DOACs (apixaban, edoxaban, and rivaroxaban), and the direct thrombin inhibitor argatroban.

Information:

 3:15 p.m. - 3:35 p.m.

 06 April 2022

 De Anza 1 Room

Authors:

1. Briana Fitch, 2; German A. Gómez-Ríos,1; Kara Lynch, 2

Affiliations:

1. Restek Corporation, 2. University of California

Presenters:

Briana Fitch

 

Measurement of methotrexate (MTX) in patients receiving high doses of MTX is important due to the risk of toxicity and wide variations in clearance between different patients receiving the same dose. Glucarpidase therapy is recommended when clearance of MTX is delayed. Typically, this enzyme inactivates MTX by cleaving it into two nontoxic metabolites: DAMPA and 7OH-MTX. It is well known that these metabolites interfere with commonly used MTX immunoassays, which means the assays can offer inaccurate and potentially misleading results. Therefore, more specific methods, such as those based on mass spectrometry, are preferred for the determination of MTX in patients under Glucarpidase therapy. Whilst several LC-MS/MS methods for MTX have been previously developed, these tend to require long chromatography times or complex sample prep workflows. Thus, having faster and specific assays for MTX and metabolites is ideal. Coated Blade Spray (CBS) is an open-bed SPE technology that can be directly interfaced with mass spectrometry (MS) instrumentation for rapid screening and quantitation of drugs and metabolites in biofluids.

Information:

 3:35 p.m. - 3:55 p.m.

 06 April 2022

 De Anza 1 Room

Authors:

1. German Gómez

Affiliations:

Restek Corporation

Presenters:

German Gómez

 

Amino acids are critical molecules for biological function as they serve as building blocks of peptides/proteins and intermediates of various metabolic pathways (e.g., urea cycle and citric acid cycle). Amino acids profiling in plasma is an important tool for the diagnosis of metabolic disorders, especially for inborn errors of metabolism. A fast-and-accurate, large-panel amino acids analysis is critical not only for disorder identification but also for continued monitoring and assessment of nutritional status of patients and treatment plans. Traditionally, amino acids were analyzed by either post-column or precolumn derivatization methodologies. The major shortcomings of these approaches are long chromatographic cycling time (post-column derivatization) and poor chromatographic separation (precolumn derivatization) for comprehensive amino acid quantification in plasma. The relative lack of applications for direct analysis of underivatized amino acids by LC-MS/MS is due to limited chromatographic retention and insufficient detection sensitivity.

Information:

 11:00 a.m. - 12:00 p.m.

 06 April 2022

 Exhibit Hall

Authors:

1. Shun-Hsin Liang, Amol Kafle

Affiliations:

Restek Corporation

Presenters:

Amol Kafle

 

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Efficiency is key when running multiple drug panels, and any way to save time or streamline a process can help reduce costs. To help simplify the analysis of alcohol metabolites, barbiturates, and drugs of abuse, three different methods were developed for each analyte class using the same analytical column and mobile phase setup. A panel of 129 drug and drug metabolite isobars in positive mode, negative mode drug and drug metabolites, and alcohol metabolites were all analyzed using a Force Biphenyl 50 x 3 mm, 2.7 µm analytical column and 0.1% formic acid in water and 0.1% formic acid in methanol mobile phases. The Force Biphenyl phase has unique selectivity due to the pi-pi interactions for drugs and drug metabolites when compared to a routine C18 phase allowing for improved resolution of isobars. Urinary interferences that are particularly problematic in alcohol metabolite testing were resolved without the use of buffer or additional mobile phases helping to streamline analytical testing processes.

Information:

 1:00 p.m. - 2:00 p.m.

 07 April 2022

 Exhibit Hall

Authors:

1. Jamie York, Jonathan Edelman

Affiliations:

Restek Corporation

Presenters:

German Gomez

 

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