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Chiral Separations 5: Separation with Rt-ßDEXsa, Rt-ßDEXcst and Rt-γDEXsa

12 November 2021
By
  • Jana Hepner
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In this blog we’ll continue looking into individual columns and specific separations. We’ll focus on Rt-βDEXsa, Rt-βDEXcst and Rt-γDEXsa (Table 1), which all have somewhat different selectivity from the columns discussed in the last blog (Rt-βDEXm, Rt-βDEXsm and Rt-βDEXse).

Table 1: Resolution of selected chiral compounds by Rt-βDEXsa, Rt-βDEXcst and Rt-γDEXsa
 

Rt-ßDEXsa

Rt-ßDEXcst

Rt-γDEXsa

1-octen-3-ol

2.00

ns

1.35

β-citronellol

0.98

ns

ns

menthol

0.93

0.89

2.89

camphor

4.20

2.22t

3.64

α-ionone

4.69

1.37

8.43

γ-nonalactone

4.00

3.82

0.99

γ-undecalactone

3.65

3.18

0.84

δ-decalactone

1.91

1.69

0.75

Chiral Separations with Rt-βDEXsa

The Rt-bDEXsa has a significantly different selectivity than the other chiral columns. It provides the best separation of 1-octen-3-ol, carvone, camphor, b-citronellol (Figure 1A-D, respectively), and rose oxides (Figure 2).

blog-chiral-blog-5-01.png

Figure 1: Separation of A) 1-octen-3-ol, B) β-citronellol, C) carvone and D) Camphor on Rt-bDEXsa

blog-chiral-blog-5-02.png

Figure 2: Separation of Rose oxides on Rt-bDEXsa

Chiral Separation with Rt-ßDEXcst

This column is optimum for semivolatile chiral compounds. It provides good resolution of the irone isomers (found in iris flowers; Figure 3). This is also suitable column for resolution of the γ- and δ-lactones (Figure 4).

blog-chiral-blog-5-03.png

Figure 3: Separation of irone isomers on Rt-ßDEXcst

blog-chiral-blog-5-04.png

Figure 4: Separation of lactones on Rt-ßDEXcst

Chiral Separations with Rt-γDEXsa

The Rt-γDEXsa is unique in the Restek chiral column selection due to doping of γ-cyclodextrins into the stationary phase polymer. The γ-cyclodextrins have a larger cavity; therefore, this column is useful for larger organic molecules. While our study was focused more on small molecules, the Rt-γDEXsa had very good separation for menthol and α-ionone (Figure 5).

blog-chiral-blog-5-05.png

Figure 5: Separation of A) menthol and B) α-Ionone on Rt- γDEXsa

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