Cocaine and Metabolites (TMS Derivatives) on Rxi-5Sil MS (100 ng/mL)

	Peaks	m/z 1	m/z 2	m/z 3
1.	Ecgonine methyl ester	82	96	256
2.	Ecgonine	82	96	356
3.	Cocaine	82	182	303
4.	Cocaethylene	82	196	317
5.	Benzoylecgonine	82	96	346
6.	Cannabinol*	409	--	--

Detector	MS
Mode:	SIM
Transfer Line Temp.:	310 °C
Source Temp.:	250 °C
Solvent Delay Time:	4 min
Tune Type:	PFTBA
Ionization Mode:	EI
Instrument	Shimadzu 2010 GC & QP2010+ MS
Notes	Samples were prepared as follows: Standards brought to dryness under nitrogen, then 50 µL BSTFA + 1% TMCS (cat.# 35606) were added. 50 µL pyridine was then added, and samples were incubated at 70 °C for 30 min. After incubation, samples were diluted with butyl chloride. * Cannabinol was used as a derivatization check.

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