Extracted Opiates from Blood on Rxi-5Sil MS

* Matrix

Standard/Sample
Column	Rxi-5Sil MS, 30 m, 0.25 mm ID, 0.25 µm (cat.# 13623)
Diluent:	Ethyl acetate
Conc.:	100 ng/mL propionic anhydride derivatives
Injection
Inj. Vol.:	1 µL splitless (hold 1 min)
Liner:	4 mm splitless taper w/wool (cat.# 22405)
Inj. Temp.:	250 °C
Purge Flow:	100 mL/min
Oven
Oven Temp.:	65 °C (hold 1 min) to 315 °C at 15 °C/min
Carrier Gas	He, constant flow
Flow Rate:	1 mL/min
Linear Velocity:	35 cm/sec @ 65 °C

Detector	MS
Mode:	SIM
Transfer Line Temp.:	250 °C
Analyzer Type:	Quadrupole
Solvent Delay Time:	7 min
Tune Type:	PFTBA
Ionization Mode:	EI
Sample Preparation	Opiates were spiked into a blood sample and extracted by SPE, then derivatized with propionic anhydride.
Acknowledgement	Data courtesy of Miami Dade County Medical Examiner Department

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