Blood Alcohol Analysis on Rtx-BAC Plus 1

Oven
Column	Rtx-BAC Plus 1, 30 m, 0.32 mm ID, 1.8 µm (cat.# 18004)
Standard/Sample	BAC resolution control standard n-P (cat.# 36010)
	BAC resolution control standard t-B (cat.# 36011)
Diluent:	Water
Conc.:	50 µL of each standard were diluted in 900 µL water in a 20 mL headspace vial.
Injection	headspace-loop split (split ratio 50:1)
Liner:	1 mm ID straight inlet liner (cat.# 20972)
Headspace-Loop
Inj. Port Temp.:	200 °C
Instrument:	Tekmar HT3
Inj. Time:	3 min
Transfer Line Temp.:	125 °C
Valve Oven Temp.:	125 °C
Standby flow rate:	50 mL/min
Sample Temp.:	60 °C
Sample Equil. Time:	5 min
Vial Pressure:	30 psi
Pressurize Time:	1 min
Loop Pressure:	20 psi
Loop Fill Time:	1 min
Oven Temp.:	40 °C (hold 3 min)
Carrier Gas	He, constant flow
Linear Velocity:	80 cm/sec @ 40 °C

Detector	FID @ 240 °C
Make-up Gas Flow Rate:	30 mL/min
Make-up Gas Type:	N₂
Instrument	Agilent/HP6890 GC
Notes	Headspace concentrator courtesy of Teledyne Tekmar, Mason, OH.

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