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Comparison of 2,4-Dinitrophenol response with Semivolatiles wool and MS Certified wool

ColumnRxi-5Sil MS, 30 m, 0.25 mm ID, 0.25 µm (cat.# 13623)
SamplePhenols/Anilines/Pesticides Test Mix (cat.# 35245)
Conc.:10 ng on-column
Inj. Vol.:1.0 µL splitless (hold 0.75 min)
Liner:Gooseneck Splitless (4mm) w/Semivolatiles Wool (cat.# 20798-231.1)
Inj. Temp.:250 °C
Oven Temp.:50 °C (hold 3.5 min) to 180 °C at 35 °C/min (hold 3.5 min) to 300 °C at 20 °C/min (hold 0.5 min)
Carrier GasHe, constant flow
Flow Rate:1.2 mL/min
DetectorFID @ 330 °C
Hydrogen: 30mL/min.
Air: 400mL/min.
Nitrogen: 25mL/min.

10ng 2,4-dinitrophenol (DNP) on-column, single gooseneck liner with wool, FID analysis with phenanthrene internal standard.