9-Minute Semivolatiles Analysis with Appendix IX on Rxi-5ms by U.S. EPA Method 8270D

	Peaks
1.	1,4-Dioxane-d8 (IS)
2.	1,4-Dioxane
3.	N-Nitrosodimethylamine
4.	Pyridine
5.	Ethyl methacrylate
6.	2-Picoline
7.	N-Nitrosomethylethylamine
8.	Methyl methanesulfonate
9.	2-Fluorophenol (SS)
10.	N-Nitrosodiethylamine
11.	Ethyl methanesulfonate
12.	Benzaldehyde
13.	Phenol-d6 (SS)
14.	Phenol
15.	Aniline
16.	Pentachloroethane
17.	Bis(2-chloroethyl) ether
18.	2-Chlorophenol
19.	1,3-Dichlorobenzene
20.	1,4-Dichlorobenzene-d4 (IS)
21.	1,4-Dichlorobenzene
22.	Benzyl alcohol
23.	1,2-Dichlorobenzene
24.	2-Methylphenol
25.	Bis(2-Chloroisopropyl)ether
26.	p-Cresol (4-methylphenol)
27.	m-Cresol (3-methylphenol)
28.	Acetophenone
29.	N-Nitrosopyrrolidine
30.	N-Nitrosomorpholine
31.	N-Nitrosodi-n-propylamine
32.	o-Toluidine
33.	Hexachloroethane
34.	Nitrobenzene-d5 (SS)
35.	Nitrobenzene
36.	N-Nitrosopiperidine
37.	Isophorone
38.	2-Nitrophenol
39.	2,4-Dimethylphenol
40.	Bis(2-chloroethoxy)methane
41.	Benzoic acid
42.	2,4-Dichlorophenol
43.	α,α-Dimethylphenethylamine (Phentermine)
44.	1,2,4-Trichlorobenzene
45.	Naphthalene-d8 (IS)
46.	Naphthalene
47.	4-Chloroaniline
48.	2,6-Dichlorophenol

	Peaks
49.	Hexachloropropene
50.	Hexachlorobutadiene
51.	Caprolactam
52.	N-Nitroso-n-butylamine
53.	1,4-Phenylenediamine
54.	4-Chloro-3-methylphenol
55.	Isosafrole I (isomer)
56.	2-Methylnaphthalene
57.	1-Methylnaphthalene
58.	Isosafrole II (isomer)
59.	1,2,4,5-Tetrachlorobenzene
60.	Hexachlorocyclopentadiene
61.	2,4,6-Trichlorophenol
62.	2,4,5-Trichlorophenol
63.	2-Fluorobiphenyl (SS)
64.	Safrole
65.	Biphenyl
66.	2-Chloronaphthalene
67.	1-Chloronaphthalene
68.	Diphenyl ether
69.	2-Nitroaniline
70.	1,4-Naphthoquinone
71.	1,4-Dinitrobenzene
72.	Dimethyl phthalate
73.	1,3-Dinitrobenzene
74.	2,6-Dinitrotoluene
75.	Acenaphthylene
76.	1,2-Dinitrobenzene
77.	3-Nitroaniline
78.	Acenaphthene-d10 (IS)
79.	Acenaphthene
80.	2,4-Dinitrophenol
81.	4-Nitrophenol
82.	Dibenzofuran
83.	Pentachlorobenzene
84.	2,4-Dinitrotoluene
85.	1-Naphthalamine
86.	2,3,5,6-Tetrachlorophenol
87.	2-Naphthalamine
88.	2,3,4,6-Tetrachlorophenol
89.	Diethyl phthalate
90.	4-Chlorophenyl phenyl ether
91.	Fluorene
92.	2-Methyl-5-nitroaniline
93.	4-Nitroaniline
94.	4,6-Dinitro-2-methylphenol
95.	N-Nitrosodiphenylamine
96.	1,2-Diphenylhydrazine (as Azobenzene)

	Peaks
97.	2,4,6-Tribromophenol (SS)
98.	1,3,5-Trinitrobenzene
99.	Diallate
100.	Phenacetin
101.	4-Bromophenyl phenyl ether
102.	Hexachlorobenzene
103.	Atrazine
104.	4-Aminobiphenyl
105.	Pentachlorophenol
106.	Propyzamide
107.	Pentachloronitrobenzene
108.	Phenanthrene-d10 (IS)
109.	Phenanthrene
110.	Anthracene
111.	Carbazole
112.	di-n-Butyl phthalate
113.	4-Nitroquinoline oxide
114.	Isodrin
115.	Fluoranthene
116.	Benzidine
117.	Pyrene-d10 (SS)
118.	Pyrene
119.	Aramite I (isomer)
120.	p-Terphenyl-d14 (SS)
121.	Aramite II (isomer)
122.	Dimethylaminoazobenzene
123.	Chlorobenzilate
124.	3,3'-Dimethylbenzidine
125.	Kepone
126.	n-Butyl benzyl phthalate
127.	Bis(2-ethylhexyl) adipate
128.	2-Acetylaminofluorine
129.	3,3'-Dichlorobenzidine
130.	Chrysene-d12 (IS)
131.	Benz[a]anthracene
132.	Chrysene
133.	Bis(2-ethylhexyl) phthalate
134.	Di-n-octyl phthalate
135.	Benzo[b]fluoranthene
136.	Benzo[k]fluoranthene
137.	7,12-Dimethylbenzo[a]anthracene
138.	Benzo[a]pyrene
139.	Perylene-d12 (IS)
140.	3-Methylcholanthrene
141.	Dibenz[a,j]acridine
142.	Indeno[1,2,3-cd]pyrene
143.	Dibenz[a,h]anthracene
144.	Benzo[ghi]perylene

Injection
Column	Rxi-5ms, 10 m, 0.18 mm ID, 0.18 µm
	using Rxi-5ms 2 m, 0.18 mm ID,
	with 0.4 mm OD matched press-fit, BGB (cat.# 2525LD)
Standard/Sample	Revised SV internal standard mix (cat.# 31886)
	Revised B/N surrogate mix (cat.# 31888)
	Acid surrogate mix (4/89 SOW) (cat.# 31063)
	8270 MegaMix (cat.# 31850)
	8270 Benzidines mix (cat.# 31852)
	Benzoic acid (cat.# 31879)
	Appendix IX mix #1, revised (cat.# 32459)
	Appendix IX mix #2 (cat.# 31806)
Diluent:	Dichloromethane
Conc.:	20 µg/mL (1.0 ng on column)
Inj. Vol.:	0.5 µL split (split ratio 10:1)
Liner:	2 mm Split Precision liner w/deact. wool (cat.# 20824)
Inj. Temp.:	275 °C
Split Vent Flow Rate:	9 mL/min
Oven
Oven Temp.:	35 °C (hold 0.5 min) to 115 °C at 95 °C/min to 240 °C at 65 °C/min to 265 °C at 6 °C/min to 330 °C at 35 °C/min (hold 1 min)
Carrier Gas	He, constant flow
Flow Rate:	0.9 mL/min

Detector

Mode:

Scan

Scan Program:

Group	Start Time (min)	Scan Range (amu)	Scan Rate (scans/sec)
1	0.75	35-450	11.47
2	3.67	35-450	6.48

Transfer Line Temp.:

320 °C

Analyzer Type:

Quadrupole

Source Type:

Inert

Drawout Plate:

3mm ID

Source Temp.:

330 °C

Quad Temp.:

180 °C

Electron Energy:

70 eV

Solvent Delay Time:

0.75 min

Tune Type:

BFB

Ionization Mode:

Instrument

Agilent 7890A GC & 5975C MSD

Notes

The 10 m analytical and 2 m guard columns used in this application are segments of a 20 m x 0.18 mm ID x 0.18 µm df Rxi-5ms column (cat.# 13402). The 20 m column was cut to yield one 10 m analytical column and five 2 m guard columns.

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