FAMEs PUFA (menhaden source) FAMEWAX

GC_FF00115

	Peaks
1.	C14:0
2.	C16:0
3.	C16:1n7
4.	C16:2n4
5.	C16:3n4
6.	C16:4n1
7.	C18:0
8.	C18:1n9
9.	C18:1n7
10.	C18:1n5
11.	C18:2n6
12.	C18:2n4

	Peaks
13.	C18:3n6
14.	C18:3n4
15.	C18:3n3
16.	C18:4n3
17.	C18:4n1
18.	C20:1n15
19.	C20:1n9
20.	C20:1n7
21.	C20:2n6
22.	C20:3n6
23.	C20:4n6
24.	C20:3n3

	Peaks
25.	C20:4n3
26.	C20:5n3
27.	C22:1n9
28.	C22:2n6
29.	C21:5n3
30.	C23:0 (IS)
31.	C22:4n6
32.	C22:5n6
33.	C22:5n3
34.	C24:0
35.	C22:6n3
36.	C24:1n9

Injection
Column	FAMEWAX, 30 m, 0.25 mm ID, 0.25 µm (cat.# 12497)
Standard/Sample	menhaden oil
Conc.:	100-150 ng
Inj. Vol.:	0.8 µL split (split ratio 50:1)
Inj. Temp.:	220 °C
Oven
Oven Temp.:	120 °C to 220 °C at 7 °C/min (hold 20 min)
Carrier Gas	He, constant flow
Linear Velocity:	60 cm/sec @ 120 °C

Detector	FID @ 220 °C
Notes	FID sensitivity: 8 x 10-11 AFS

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