Underivatized Cholesterol on Rxi-5ms (Temp. program)

	Peaks
1.	5-α-Cholestane (IS)
2.	Cholesterol

Injection
Column	Rxi -5ms, 15 m, 0.25 mm ID, 0.25 µm (cat.# 13420)
Standard/Sample	1,000 μg/mL cholesterol in DMF
	1,000 μg/mL 5-α-cholestane in hexane
	25 ng cholesterol, 150 ng 5-α-cholestane on column
Inj. Vol.:	1 µL split (split ratio 20:1)
Liner:	Splitless taper (4 mm) w/wool (cat.# 22405)
Inj. Temp.:	250 °C
Oven
Oven Temp.:	200 °C (hold 1 min) to 330 °C at 20 °C/min (hold 7.5 min)
Carrier Gas	He, constant pressure (9.7 psi, 66.9 kPa)
Linear Velocity:	24 cm/sec @ 200 °C

Detector	FID @ 340 °C

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