Analysis of Melamine and Related Compounds in Infant Formula on Rxi-5Sil MS

	Peaks	t_R (min)	Quant. Ion	Qual. Ion	Qual.Ion	Qual. Ion
1.	Cyanuric acid	10.258	345	330	346	347
2.	Ammelide	11.073	344	329	345	330
3.	Ammeline	11.760	328	343	329	344
4.	Melamine	12.328	327	342	328	343
5.	Benzoguanamine	14.538	316	331	332	330

Injection
Column	Rxi-5Sil MS w/5 m Integra-Guard column, 30 m, 0.25 mm ID, 0.25 µm (cat.# 13623-124)
Sample	Infant formula fortified at 1 μg/g with melamine and related analogs stock standard (cat.# 33253)
	Benzoguanamine (cat.# 33251)
Conc.:	0.01 µg/mL as tri-TMS derivatives
Inj. Vol.:	1.0 µL splitless (hold 1 min)
Liner:	3.5 mm Gooseneck Splitless w/Wool (cat.# 22286-200.1)
Inj. Temp.:	280 °C
Oven
Oven Temp.:	75 °C to 320 °C at 15 °C/min (hold 4 min)
Carrier Gas	He, constant flow
Flow Rate:	1 mL/min

Detector	MS
Mode:	SIM
Transfer Line Temp.:	290 °C
Ionization Mode:	EI
Instrument	Shimadzu 2010 GC & QP2010+ MS
Notes	all method ions in table, only quantification ions were plotted

Download PDF