QuEChERS Extract of NIST SRM 2974a Freeze-Dried Mussel Tissue (GCxGC contour plot)

Injection
Column	Rxi-17Sil MS 30 m, 0.25 mm ID, 0.25 µm (cat.# 14123)
	Rxi-1ms 1.2 m, 0.15 mm ID, 0.15 µm (cat.# custom)

Standard/Sample	NIST SRM 2974a freeze-dried mussel tissue with incurred residues
Diluent:	Acetonitrile
Inj. Vol.:	1 µL splitless (hold 1 min)
Liner:	Splitless taper (4 mm) w/semivolatiles wool (cat.# 20798-231.1)
Inj. Temp.:	250 °C
Purge Flow:	40 mL/min
Oven
Oven Temp.:	Rxi-17Sil MS: 90 °C (hold 1 min) to 320 °C at 3.75 °C/min (hold 2.67 min) Rxi-1ms: 95 °C (hold 1 min) to 325 °C at 3.75 °C/min (hold 2.67 min)
Carrier Gas	He, corrected constant flow (2.2 mL/min)
Modulation
Modulator Temp. Offset:	20 °C
Second Dimension Separation Time:	3 sec
Hot Pulse Time:	0.9 sec
Cool Time between Stages:	0.6 sec

Detector	TOFMS
Transfer Line Temp.:	300 °C
Analyzer Type:	TOF
Source Temp.:	250 °C
Electron Energy:	70 eV
Mass Defect:	-20 mu/100 u
Solvent Delay Time:	5 min
Tune Type:	PFTBA
Ionization Mode:	EI
Acquisition Range:	45 to 550 amu
Spectral Acquisition Rate:	100 spectra/sec
Instrument	LECO Pegasus 4D GCxGC-TOFMS
Notes	PAHs (shown in box) elute in a relatively narrow band and are identified by the white peak marker dots.

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