Marijuana Pesticides by GCxGC on Rxi-5Sil MS and Rtx-200

	Peaks	RT 1 (sec)	RT 2 (sec)
1.	Methamidophos	480	2.51
2.	Dichlorvos	507	2.11
3.	1,2,3,5-Tetrachlorobenzene	621	1.12
4.	Nicotine	654	1.39
5.	Mevinphos	768	2.59
6.	Acephate	771	0.17
7.	Pentachlorobenzene	885	1.16
8.	o-Phenylphenol	888	1.21
9.	Tebuthiuron	894	2.06
10.	Tetrachloronitrobenzene (Tecnazene)	984	1.59
11.	Omethoate	990	0.25
12.	2,3,5,6-Tetrachloroaniline	1029	1.28
13.	α-BHC	1128	1.40
14.	Hexachlorobenzene	1134	1.16
15.	Pentachloroanisole	1152	1.26
16.	Dimethoate	1164	2.46
17.	β-BHC	1191	1.64
18.	Pentachloronitrobenzene	1197	1.52
19.	Pentachlorobenzonitrile	1206	1.69
20.	γ-BHC	1212	1.43
21.	Chlorothalonil	1251	2.56
22.	Anthracene	1257	1.42
23.	Diazinon	1260	1.25
24.	δ-BHC	1281	1.49
25.	Pentachloroaniline	1314	1.30
26.	Vinclozolin	1371	2.08

	Peaks	RT 1 (sec)	RT 2 (sec)
27.	Carbaryl	1383	2.06
28.	Metalaxyl	1398	1.87
29.	Pentachlorothioanisole	1431	1.20
30.	Pirimiphos methyl	1434	1.33
31.	Methiocarb	1437	1.59
32.	Dichlofluanid	1446	2.00
33.	Malathion	1461	1.79
34.	Chlorpyrifos	1473	1.38
35.	Fenthion	1479	1.43
36.	DCPA methyl ester (Chlorthal-dimethyl)	1482	1.71
37.	Parathion	1488	2.10
38.	Cyprodinil	1548	1.20
39.	Heptachlor epoxide	1557	1.38
40.	Thiabendazole	1572	1.47
41.	Captan	1578	1.83
42.	Folpet	1590	1.74
43.	Procymidone	1590	2.24
44.	Endosulfan I	1638	1.41
45.	Imazalil	1674	1.88
46.	4,4'-DDE	1695	1.17
47.	Dieldrin	1698	1.40
48.	Myclobutanil	1707	2.22
49.	Endrin	1740	1.44
50.	Endosulfan II	1767	1.55
51.	4,4'-DDD	1785	1.31
52.	Oxadixyl	1785	2.94

Standard/Sample
Column	Rxi-5Sil MS 30 m, 0.25 mm ID, 0.25 µm (cat.# 13623)
	Rtx-200 1.3 m, 0.25 mm ID, 0.25 µm (cat.# 15020)

Diluent:	Toluene
Injection
Inj. Vol.:	1 µL splitless (hold 1 min)
Liner:	Premium 4 mm single taper w/wool (cat.# 23303.1)
Inj. Temp.:	250 °C
Purge Flow:	40 mL/min
Oven
Oven Temp.:	Rxi-5Sil MS: 80 °C (hold 1 min) to 310 °C at 5 °C/min Rtx-200: 85 °C (hold 1 min) to 315 °C at 5 °C/min
Carrier Gas	He, corrected constant flow (2 mL/min)
Modulation
Modulator Temp. Offset:	20 °C
Second Dimension Separation Time:	3 sec
Hot Pulse Time:	0.9 sec
Cool Time between Stages:	0.6 sec

Detector	TOFMS
Transfer Line Temp.:	290 °C
Analyzer Type:	TOF
Source Temp.:	225 °C
Electron Energy:	70 eV
Mass Defect:	-20 mu/100 u
Solvent Delay Time:	5 min
Tune Type:	PFTBA
Ionization Mode:	EI
Acquisition Range:	45-550 amu
Spectral Acquisition Rate:	100 spectra/sec
Instrument	LECO Pegasus 4D GCxGC-TOFMS
Notes	Rtx-200 (cat.# 15020) is a 15 m column. A 1.3 m section was used as the second dimension column.

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