GCxGC-TOFMS Analysis of Multi-Pesticide Standard Using Rxi-5Sil MS and Rtx-200

	Peaks
1.	Methamidophos
2.	Dichlorvos
3.	1,2,3,5-Tetrachlorobenzene
4.	Mevinphos
5.	Acephate
6.	Pentachlorobenzene
7.	o-Phenylphenol
8.	Tetrachloronitrobenzene (Tecnazene)
9.	Omethoate
10.	2,3,5,6-Tetrachloroaniline
11.	Alpha-BHC
12.	Hexachlorobenzene
13.	Pentachloroanisole
14.	Dimethoate
15.	Beta-BHC
16.	Pentachloronitrobenzene
17.	Pentachlorobenzonitrile
18.	Gamma-BHC
19.	Chlorothalonil
20.	Anthracene
21.	Diazinon
22.	delta-BHC
23.	Pentachloroaniline
24.	Vinclozolin

	Peaks
25.	Carbaryl
26.	Metalaxyl
27.	Pentachlorothioanisole
28.	Pirimiphos methyl
29.	Methiocarb
30.	Dichlofluanid
31.	Malathion
32.	Chlorpyrifos
33.	Fenthion
34.	DCPA methyl ester (Chlorthal-dimethyl)
35.	Parathion
36.	Cyprodinil
37.	Heptachlor epoxide
38.	Thiabendazole
39.	Captan
40.	Folpet
41.	Procymidone
42.	Endosulfan I
43.	Imazalil
44.	4,4'-DDE
45.	Dieldrin
46.	Myclobutanil
47.	Endrin
48.	Endosulfan II

Injection
Column	Rxi-5Sil MS 30 m, 0.25 mm ID, 0.25 µm (cat.# 13623)
	Rtx-200 1.3 m, 0.25 mm ID, 0.25 µm (cat.# 15124)

Sample	Custom pesticide standard
Diluent:	Acetonitrile
Conc.:	500 pg/µL
Inj. Vol.:	1.0 µL splitless (hold 1.0 min)
Liner:	Premium 4 mm single taper w/wool (cat.# 23303.5)
Inj. Temp.:	250 °C
Oven
Oven Temp.:	Rxi-5Sil MS: 90 °C (hold 1.0 min) to 310 °C at 5 °C/min (hold 2.0 min) Rtx-200: 95 °C (hold 1.0 min) to 315 °C at 5 °C/min (hold 2.0 min)
Carrier Gas	He, corrected constant flow (2 mL/min)
Modulation
Modulator Temp. Offset:	+20 °C
Second Dimension Separation Time:	3 sec
Hot Pulse Time:	0.9 sec
Cool Time between Stages:	0.6 sec

Detector	MS
Mode:
Transfer Line Temp.:	300 °C
Analyzer Type:	TOF
Source Temp.:	225 °C
Electron Energy:	70 eV
Mass Defect:	-20 mu/100 u
Ionization Mode:	EI
Acquisition Range:	45 to 550 amu
Spectral Acquisition Rate:	100 spectra/sec
Instrument	LECO Pegasus 4D GCxGC-TOFMS
Notes	Rtx-200 (cat.# 15124) is a 2 m column. A 1.3 m section was cut off and used as the second dimension column.

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