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Nutritional Labeling FAMEs on Rt-2560 by AOAC Method 996.06

PeakstR (min)Conc.
Structural Nomenclature
1.Methyl butyrate13.1640C4:0
2.Methyl caproate15.5340C6:0
3.Methyl octanoate19.4340C8:0
4.Methyl decanoate24.5040C10:0
5.Methyl undecanoate27.2020C11:0
6.Methyl dodecanoate29.8740C12:0
7.Methyl tridecanoate32.4720C13:0
8.Methyl myristate34.9740C14:0
9.Methyl myristoleate37.0120C14:1 (c9)
10.Methyl pentadecanoate37.3720C15:0
11.Methyl pentadecenoate39.3620C15:1 (c10)
12.Methyl palmitate39.6860C16:0
13.Methyl palmitoleate41.3020C16:1 (c9)
14.Methyl heptadecanoate41.8620C17:0
15.Methyl heptadecenoate43.4520C17:1 (c10)
16.Methyl stearate43.9740C18:0
17.Methyl octadecenoate44.9220C18:1 (t9)
18.Methyl oleate45.3440C18:1 (c9)
PeakstR (min)Conc.
Structural Nomenclature
19.Methyl linolelaidate46.3920C18:2 (t9,t12)
20.Methyl linoleate47.3220C18:2 (c9,c12)
21.Methyl arachidate47.9240C20:0
22.Methyl linolenate48.7720C18:3 (c6,c9,c12)
23.Methyl eicosenoate49.2020C20:1 (c11)
24.Methyl linolenate 49.5420C18:3 (c9,c12,c15)
25.Methyl heneicosanoate49.7820C21:0
26.Methyl eicosadienoate51.0920C20:2 (c11,c14)
27.Methyl behenate51.6040C22:0
28.Methyl eicosatrienoate 52.5220C20:3 (c8,c11,c14)
29.Methyl erucate52.8820C22:1 (c13)
30.Methyl eicosatrienoate 53.2820C20:3 (c11,c14,c17)
31.Methyl arachidonate53.4220C20:4 (c5,c8,c11,c14)
32.Methyl tricosanoate53.6520C23:0
33.Methyl docosadienoate54.8520C22:2 (c13,c16)
34.Methyl lignocerate55.3240C24:0
35.Methyl eicosapentaenoate56.0920C20:5 (c5,c8,c11,c14,c17)
36.Methyl nervonate56.7420C24:1 (C15)
37.Methyl docosahexaenoate62.1720C22:6 (c4,c7,c10,c13,c16,c19)
ColumnRt-2560, 100 m, 0.25 mm ID, 0.20 µm (cat.# 13198)
Standard/SampleFood industry FAME mix (cat.# 35077)
Conc.:1,000 µg/mL
Inj. Vol.:1 µL split (split ratio 20:1)
Liner:Premium 4 mm Precision liner w/wool (cat.# 23305.1)
Inj. Temp.:225 °C
Oven Temp.:100 °C (hold 4 min) to 240 °C at 3 °C/min (hold 15 min)
Carrier GasHe, constant flow
Flow Rate:1.0 mL/min
DetectorFID @ 285 °C
Make-up Gas Flow Rate:45 mL/min
Make-up Gas Type:N2
Hydrogen flow:30 mL/min
Air flow:300 mL/min
Data Rate:20 Hz
InstrumentAgilent 7890A GC