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Olive Oil FAMEs on Stabilwax

PeakstR (min)Conc.
Structural Nomenclature
1.Methyl palmitate15.25611.9C16:0
2.Methyl palmitoleate15.6461.0C16:1 (cis-9)
3.Methyl stearate18.2443.2C18:0
4.Methyl oleate18.63173.2C18:1 (cis-9)
5.Methyl linoleate19.2238.9C18:2 (cis-9,12)
6.Methyl linolenate20.1140.8C18:3 (cis-9,12,15)
7.Methyl arachidate20.9850.5C20:0
8.Methyl eicosenoate21.2870.4C20:1 (cis-11)
ColumnStabilwax, 60 m, 0.25 mm ID, 0.25 µm (cat.# 10626)
Standard/SampleOlive oil reference standard (Sigma-Aldrich #47118)
Inj. Vol.:1 µL split (split ratio 100:1)
Liner:Topaz 4 mm ID straight inlet liner w/ wool (cat.# 23300)
Inj. Temp.:225 °C
Oven Temp.:120 °C (hold 0.5 min) to 250 °C at 6 °C/min (hold 15 min)
Carrier GasH2, constant flow
Flow Rate:2 mL/min
DetectorFID @ 250 °C
InstrumentAgilent 7890A GC
Sample PreparationThe sample was prepared using methanolic HCl.