Olive Oil FAMEs on Stabilwax

	Peaks	t_R (min)	Conc. (wt.%)	Structural Nomenclature
1.	Methyl palmitate	15.256	11.9	C16:0
2.	Methyl palmitoleate	15.646	1.0	C16:1 (cis-9)
3.	Methyl stearate	18.244	3.2	C18:0
4.	Methyl oleate	18.631	73.2	C18:1 (cis-9)
5.	Methyl linoleate	19.223	8.9	C18:2 (cis-9,12)
6.	Methyl linolenate	20.114	0.8	C18:3 (cis-9,12,15)
7.	Methyl arachidate	20.985	0.5	C20:0
8.	Methyl eicosenoate	21.287	0.4	C20:1 (cis-11)

Injection
Column	Stabilwax, 60 m, 0.25 mm ID, 0.25 µm (cat.# 10626)
Sample	Olive oil reference standard (Sigma-Aldrich #47118)
Diluent:	Hexane
Inj. Vol.:	1 µL split (split ratio 100:1)
Liner:	Topaz 4 mm ID straight inlet liner w/ wool (cat.# 23300)
Inj. Temp.:	225 °C
Oven
Oven Temp.:	120 °C (hold 0.5 min) to 250 °C at 6 °C/min (hold 15 min)
Carrier Gas	H₂, constant flow
Flow Rate:	2 mL/min

Detector	FID @ 250 °C
Instrument	Agilent 7890A GC
Notes	The sample was prepared using methanolic HCl.

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