Rxi-65TG: High-Temperature Stability Overlay

	Peaks
1.	Tripalmitin (PPP)
2.	1,2-Palmitin-3-stearin (PPS)
3.	1,3-Palmitin-2-olein (POP)
4.	1,2-Palmitin-3-linolein (PPL)
5.	1-Palmitin-2-olein-3-stearin (POS)
6.	1,2-Olein-3-palmitin (POO)
7.	1-Palmitin-2-linolein-3-olein (PLO)
8.	1,2-Stearin-3-olein (SSO)
9.	Triolein (OOO)
10.	1,2-Olein-3-linolein (OOL)

Injection
Column	Rxi-65TG, 30 m, 0.25 mm ID, 0.1 µm (cat.# 17108)
Standard/Sample	Palm oil analytical standard
	Mono/diacylglycerol custom mix
Diluent:	Isooctane
Inj. Vol.:	1 µL split (split ratio 100:1)
Liner:	Topaz 4.0 mm ID Precision inlet liner w/wool (cat.# 23305)
Inj. Temp.:	360 °C
Split Vent Flow Rate:	200 mL/min
Oven
Carrier Gas	H₂, constant flow
Flow Rate:	2 mL/min

Detector	FID @ 360 °C
Make-up Gas Flow Rate:	30 mL/min
Make-up Gas Type:	N₂
Hydrogen flow:	40 mL/min
Air flow:	370 mL/min
Data Rate:	50 Hz
Instrument	Agilent 7890B GC
Sample Preparation	~50 mg of a palm oil standard (which contains naturally occurring triglycerides) was fortified with the following mono- and diacylglycerol standards. The mixture was then brought up to 10 mL in isooctane, giving a final mono- and diglycerides concentration of ~100 μg/mL. 1. 1,2-Dipalmitin 2. 1,3-Dipalmitin 3. 1,2-Distearin 4. 1,3-Distearin 5. 1-Monopalmitin 6. 2-Monopalmitin 7. 1-Monostearin
Notes	Six injections of fortified palm oil were made under the following conditions. Extended final hold times were used to challenge column stability. After each 24-hour challenge period, column performance was assessed using an injection with a final hold time that is typical for triglyceride analysis. Injection 1: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 12 hr) Injection 2: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 12 hr) Injection 3: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 5 min) Injection 4: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 12 hr) Injection 5: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 12 hr) Injection 6: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 5 min)

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