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Peaks | |
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1. | Tripalmitin (PPP) |
2. | 1,2-Palmitin-3-stearin (PPS) |
3. | 1,3-Palmitin-2-olein (POP) |
4. | 1,2-Palmitin-3-linolein (PPL) |
5. | 1-Palmitin-2-olein-3-stearin (POS) |
6. | 1,2-Olein-3-palmitin (POO) |
7. | 1-Palmitin-2-linolein-3-olein (PLO) |
8. | 1,2-Stearin-3-olein (SSO) |
9. | Triolein (OOO) |
10. | 1,2-Olein-3-linolein (OOL) |
Column | Rxi-65TG, 30 m, 0.25 mm ID, 0.1 µm (cat.# 17108) |
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Standard/Sample | Palm oil analytical standard |
Mono/diacylglycerol custom mix | |
Diluent: | Isooctane |
Injection | |
Inj. Vol.: | 1 µL split (split ratio 100:1) |
Liner: | Topaz 4.0 mm ID Precision inlet liner w/wool (cat.# 23305) |
Inj. Temp.: | 360 °C |
Split Vent Flow Rate: | 200 mL/min |
Oven | |
Carrier Gas | H2, constant flow |
Flow Rate: | 2 mL/min |
Detector | FID @ 360 °C |
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Make-up Gas Flow Rate: | 30 mL/min |
Make-up Gas Type: | N2 |
Hydrogen flow: | 40 mL/min |
Air flow: | 370 mL/min |
Data Rate: | 50 Hz |
Instrument | Agilent 7890B GC |
Sample Preparation | ~50 mg of a palm oil standard (which contains naturally occurring triglycerides) was fortified with the following mono- and diacylglycerol standards. The mixture was then brought up to 10 mL in isooctane, giving a final mono- and diglycerides concentration of ~100 μg/mL. 1. 1,2-Dipalmitin 2. 1,3-Dipalmitin 3. 1,2-Distearin 4. 1,3-Distearin 5. 1-Monopalmitin 6. 2-Monopalmitin 7. 1-Monostearin |
Notes | Six injections of fortified palm oil were made under the following conditions. Extended final hold times were used to challenge column stability. After each 24-hour challenge period, column performance was assessed using an injection with a final hold time that is typical for triglyceride analysis. Injection 1: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 12 hr) Injection 2: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 12 hr) Injection 3: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 5 min) Injection 4: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 12 hr) Injection 5: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 12 hr) Injection 6: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 5 min) |
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